Summary
The regeneration of monoaminergic neurons in the cerebral (supraesophageal) ganglion of the earthworm, Allolobophora caliginosa, was studied by the fluorescence method of Falck-Hillarp 3, 4, 5, and 6 weeks after extirpation of the ganglion. The monoamine fluorophores in control and regenerating cerebral ganglia were estimated by microspectrofluorimetric analysis. In the control animals catecholaminergic (noradrenergic, dopaminergic) and indolaminergic (5-HT/5-HTP) neurons were observed. Four weeks after the removal of the cerebral ganglia, two types of monoamine-containing neurons reappeared. Microspectrofluorimetrically dopaminergic and indolaminergic perikarya were identified. Reconstructions from serial sections and counts of the regenerated fluorescent cells showed no further increase in the number of these cells between the 5th and 6th postoperative weeks. In the 5- and 6-week groups the intrinsic and afferent aminergic pathways of the cerebral ganglion were reestablished.
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Supported by a fellowship from the Alexander von Humboldt Foundation, Federal Republic of Germany, 1973 (S. K.), and by a grant from the Deutsche Forschungsgemeinschaft (H.G.H.).
The authors are indebted to Professor A. Oksche for his interest in their studies, to Miss I. Lyncker for assistance with the microspectrofluorimetric recordings, to Miss D. Vaihinger for preparing the graphs, to Dr. W. Möller for sharing technical experience, and to Mr. W. Kramer for technical assistance.
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Koritsánszky, S., Hartwig, H.G. The regeneration of the monoaminergic system in the cerebral ganglion of the earthworm, Allolobophora caliginosa . Cell Tissue Res. 151, 171–186 (1974). https://doi.org/10.1007/BF00222221
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DOI: https://doi.org/10.1007/BF00222221