Abstract
Tm-2 and Tm-2a are genes conferring resistance to tomato mosaic virus in Lycopersicon esculentum. They are allelic and originated from different lines of L. peruvianum, a wild relative of tomato. In this study, random amplified polymorphic DNA (RAPD) markers linked to these genes were screened in nearly isogenic lines (NILs). To detect RAPDs differentiating NILs, 220 different 10-base oligonucleotide primers were examined by the polymerase chain reaction (PCR), and 43 of them generated 53 consistent polymorphic fragments among the NILs. Out of these 53 fragments, 13 were arbitrarily chosen and examined in respect of whether they were linked to the netted virescent (nv) gene, since nv is tightly linked to the Tm-2 locus and its phenotype is more easily distinguishable. As a result, all 13 markers were shown to be linked to nv, and hence to the Tm-2 locus. Among them, two fragments specific to the NIL carrying Tm-2 three specific to the NIL carrying Tm-2a, and four specific to both of these NILs were closely linked to nv.
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References
Alexander LJ (1963) Host-pathogen dynamics of tobacco mosaic virus on tomato. Phytopathology 61:611–617
Cirulli M, Alexander LJ (1969) Influence of temperature and strain of tobacco mosaic virus on resistance in a tomato breeding line derived from Lycopersicon peruvianum. Phytopathology 59:1287–1297
Clayberg CD (1959) The Tm-2-nv linkage. TGC Report 9:28
Foolad MR, Jones RA, Rodriguez RL (1993) RAPD markers for constructing intraspecific tomato genetic maps. Plant Cell Rep 12:293–297
Ganal MW, Young ND, Tanksley SD (1989) Pulsed-field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400
Kikuta K, Frazier WA (1947) Preliminary report on breeding tomatoes for resistance to tobacco mosaic virus. Proc Am Soc Hort Sci 49:256–262
Khush GS, Rick CM, Robinson RW (1964) Genetic activity in a heterochromatic chromosome segment of the tomato. Science 145:1432–1434
Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175
Klein-Lankhorst R, Vermunt A, Weide R, Liharska T, Zabel P (1991) Isolation of molecular markers for tomato (L. esculentum) using random amplified polymorphic DNA (RAPD). Theor Appl Genet 83:108–114
Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340
Martin GB, Ganal MW, Tanksley SD (1992) Construction of a yeast artificial library of tomato and identification of cloned segments linked to two disease resistance loci. Mol Gen Genet 233:25–32
Meshi T, Motoyoshi F, Maeda T, Yoshikawa S, Watanabe H, Okada Y (1989) Mutations in the tobacco mosaic virus 30-kDa protein gene overcome Tm-2 resistance in tomato. Plant Cell 1:515–522
Motoyoshi F, Oshima N (1975) Infection with tobacco mosaic virus of leaf mesophyll protoplasts from susceptible and resistant lines of tomato. J Gen Virol 29:81–91
Motoyoshi F, Oshima N (1977) Expression of genetically controlled resistance to tobacco mosaic virus infection in isolated tomato leaf mesophyll protoplasts. J Gen Virol 34:499–506
Murray M, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Nishiguchi M, Motoyoshi F (1987) Resistance mechanisms of tobacco mosaic virus strains in tomato and tobacco. In: Evered D, Hornett S (eds) Plant resistance to viruses. John Wiley and Sons, Chichester, pp 38–46
Pécaut P (1965) Tomate. II. Résistance au virus de la mosaïque du tabac (TMV). Rep Station d'Amél des Plantes Marâichères (INRA) 1964:50–53
Pelham J (1972) Strain-genotype interaction of tobacco mosaic virus in tomato. Ann Appl Biol 71:219–228
Rick CM (1971) Some cytogenetic features of the genome in diploid plant species. Stadler Genet Symp 1:153–174
Smith JWM, Ritchie DB (1983) A collection of near-isogenic lines of tomato: research tool of the future? Plant Mol Biol Rep 1:41–45
Soost RK (1958) Tobacco mosaic resistance. TGC Report 8:35
Soost RK (1963) Hybrid tomato resistant to tobacco mosaic virus. J Hered 54:241–244
Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 24:7213–7218
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuclei Acids Res 18:6531–6535
Williamson VM, Ho JY, Wu FF, Miller N, Kaloshian I (1994) A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato. Theor Appl Genet 87:757–763
Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segments retained around the Tm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359
Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:579–585
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Communicated by M. Koornneef
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Ohmori, T., Murata, M. & Motoyoshi, F. Identification of RAPD markers linked to the Tm-2 locus in tomato. Theoret. Appl. Genetics 90, 307–311 (1995). https://doi.org/10.1007/BF00221969
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DOI: https://doi.org/10.1007/BF00221969