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Effect of transforming growth factor beta (TGF-β) on ATP citrate lyase in isolated hepatocytes

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Summary

Transforming growth factor beta (TGF-β) activates ATP citrate lyase in freshly isolated rat liver hepatocytes in a time dependent manner. Maximal stimulation of the enzyme occurred with less than thirty minutes of incubation of the cells with TGF-β. The half maximal effect on the enzyme determined in hepatocytes incubated with TGF-β for 10 min at 37°C was elicited by TGF-β concentrations in the 10−11 – 10−12 M range. The potential role of TGF-β stimulation of ATP citrate lyase activity in new membrane synthesis is discussed.

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Authors and Affiliations

  1. Laboratory of Metabolic Regulation, Department of Food Science and Nutrition, The Ohio State University, 43210, Columbus, Ohio, USA

    Greg Reinhart & Karla Roehrig

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  1. Greg Reinhart
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  2. Karla Roehrig
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Reinhart, G., Roehrig, K. Effect of transforming growth factor beta (TGF-β) on ATP citrate lyase in isolated hepatocytes. Mol Cell Biochem 77, 121–125 (1987). https://doi.org/10.1007/BF00221920

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  • DOI: https://doi.org/10.1007/BF00221920

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