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Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor

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Abstract

We have shown that glucocorticoids induce the appearance of β2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p < 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of β2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of β2-adrenergic receptors involves synthesis of new receptor protein.

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Author notes

  1. James S. Norris

    Present address: Department of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham, Mail Slot 587, 72205, Little Rock, AR, USA

Authors and Affiliations

  1. Department of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham, Mail Slot 587, 72205, Little Rock, AR, USA

    Pamela Brown, Jeffrey Cohen, Peter O. Kohler, Stewart L. MacLeod, Keith Popovich, Robert B. Robey & Mark Sifford

  2. Department of Physiology-Biophysics, University of Arkansas for Medical Sciences, 4301 West Markham, Mail Slot 587, 72205, Little Rock, AR, USA

    James S. Norris & Lawrence E. Cornett

  3. Department of Urology, Baylor College of Medicine, 77030, Houston, TX, USA

    Allen J. Syms

  4. Department of Cell Biology, Baylor College of Medicine, 77030, Houston, TX, USA

    Roy G. Smith

Authors
  1. James S. Norris
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  2. Pamela Brown
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  3. Jeffrey Cohen
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  6. Stewart L. MacLeod
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  7. Keith Popovich
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Norris, J.S., Brown, P., Cohen, J. et al. Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor. Mol Cell Biochem 74, 21–27 (1987). https://doi.org/10.1007/BF00221909

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  • DOI: https://doi.org/10.1007/BF00221909

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