Abstract
The segregation of several isozyme marker genes has been studied in F2 inbred families from hybrids between self-sterile and five self-fertile inbred lines (nos. 2, 3, 4, 5, and 8) as well as from interline hybrids. Self-pollination of F1 hybrids between self-sterile forms and lines 5 and 8 gave an F2 segregation ratio of 1 heterozygote:1 homozygote for the gene Prx7 (chromosome 1R) against the allele from the line. This is interpreted as a result of tight linkage of the Prx7 gene with the S1 gene in chromosome 1R (recombination at a level of 0–1%). The self-pollination of such hybrids with lines 2,3 and 4 gave normal segregation for the Prx7 gene (1:2:1). This means that these lines carry a self-fertility allele which is not on chromosome 1R. Interline hybrids 5×2, 5×3 and 5×4 had self-fertility alleles for the two S genes and in inbred F2 progenies gave the expected deviating segregation for the Prx7 gene in a ratio of 2:3:1. The segregation of interline hybrid 5×8 was normal, 1:2:1, as expected. Highly-deviating segregation in an inbred F2 family of a hybrid with line 5 has also been obtained for another gene from chromosome 1R — Pgi2 (recombination with the S1 locus of 16.7%). By using the same method it has been estimated that line 4 has a self-fertility allele of the S2 locus from chromosome 2R and that the genes β-Glu and Est4/11 are linked with it (recombination 16.7% and 17.5–20% respectively). Lines 2 and 3 have a self-fertility allele of the S5 locus from chromosome 5R which is linked with the Est5-7 gene complex (recombination at a level of 28.8–36.0%).
Similar content being viewed by others
References
Voylokov AV, Fam Thanh Fuong, Smirnov VG (1993) Genetic studies of self-fertility in rye (Secale cereale L.). 1. The identification of self-fertile lines for the Sf-alleles of self-incompatibility genes. Theor Appl Genet (in press)
Gertz A, Wricke G (1989) Linkage between the incompatibility locus Z and a β-glucosidase isozyme locus (β-Glu) in rye. Plant Breed 102:255–259
Levites EV (1986) Genetics of isozymes in plants. Nauka, Novosibirsk (in Russian)
Leach CR (1988) Detection and estimation of linkage for a codominant structural gene locus linked to a gametophytic self-incompatibility locus. Theor Appl Genet 75:882–888
Lundqvist A (1956) Self-incompatibility in rye. 1. Genetic control in the diploid. Hereditas 42:293–348
Lundqvist A (1958) Self-incompatibility in rye. IV. Factors related to self-seeding. Hereditas 44:193–256
Maurer HR (1968) Disk-electrophorese. Theorie und praxis der diskontinuerlichen polyacrylamidgel-elektrophorese. Walter de Gruyter & Co. Berlin
Melz G, Schlegel R, Thiele V (1992) Genetic linkage map of rye (Secale cereale L.). Theor Appl Genet 85:33–45
Sako N, Stachmann M (1972) Multiple molecular forms of enzymes in barley leaves infected with Erysiphe graminis sp. hordei. Physiol Plant Pathol 2:217–226
Smirnov VG, Sosnikhina SP (1984) Genetics of rye. Leningrad Univ. Press., Leningrad (in Russian)
Smirnov VG, Voylokov AV (1990) Self-fertile forms of crosspollinated plants and prospects for their use in plant breeding. In: Krivchenko VI (ed) Rye Breeding (Proceedings of EUCARPIA Symposium) VIR. Leningrad, pp 171–175
Tang KS, Hart GE (1975) Use of isozymes as chromosome markers in wheat-rye addition lines and in Triticale. Genet Res Camb 26:187–201
Wehling P (1986) Genetische analyse und chromosomale lokalization von isoenzymloci beim roggen. Dissertation University of Hannover
Wehling P, Schmidt-Stohn G, Wricke G (1985) Chromosomal localization of esterase, peroxidase and phosphoglucomutase isozyme structural genes in cultivated rye (Secale cereale L.) Theor Appl Genet 70:377–382
Wricke G, Wehling P (1985) Linkage between an incompatibility locus and a peroxidase isozyme locus (Prx7) in rye. Theor Appl Genet 71:289–292
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Fuong, F.T., Voylokov, A.V. & Smirnov, V.G. Genetic studies of self-fertility in rye (Secale cereale L.). 2. The search for isozyme marker genes linked to self-incompatibility loci. Theoret. Appl. Genetics 87, 619–623 (1993). https://doi.org/10.1007/BF00221888
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00221888