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Immuno-histochemical localization of LH-RH during different phases of estrus cycle of rat, with reference to the preoptic and arcuate neurons, and the ependymal cells

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Immunohistochemical localization of luteinizing hormone-releasing hormone (LH-RH), during different phases of the estrus cycle, in the preoptic, suprachiasmatic and arcuate nuclei, and in the OVLT of rats, with special emphasis on the ependymal cells, was studied by light, fluorescent and electron microscopy, by using rabbit anti serum to synthetic LH-RH. The LH-RH neurons in the above mentioned areas, were very active during late diestrus and early proestrus phases. Specialized ependymal cells bordering the 3rd ventricle also showed varied LH-RH positive reaction during different phases of the estrus cycle. Immunofluorescent studies showed cyclic variations in the LH-RH material in the CSF of the preoptic and infundibular recesses, as well as in the 3rd ventricle near OVLT, in that, it was maximum during late diestrus and early proestrus phases. Immediately after this, the LH-RH material started reducing in these areas, and had almost vanished by the time late proestrus was reached. We have also observed that during the proestrus phase, as the LH-RH material started declining in the CSF, it had started building up in the specialized ependyma. Estrus, metaestrus and early diestrus phases showed very weak immunofluorescent LH-RH material in the lumen of the infundibular recess and in the specialized ependyma. Our immuno-electron microscopic observations showed pleomorphic LH-RH granules in the specialized ependyma during late diestrus and proestrus phases. All these observations lead us to believe that LH-RH is not synthesized in the ependymal cells, but is phagocytosed from the CSF of the 3rd ventricle by the specialized ependyma, which transports it to the ME portal system. In males, the fluorescent LH-RH material did not show any noticeable changes. With the present and previous work, it is concluded that the neurons in different nuclei synthesize LH-RH and transport it to the ME portal system, primarily through the nerve fibers and secondarily by the ventricular route. It is also sugested that the ependymal transport of LH-RH to the ME portal system is cyclic and thus controls the gonadotropin secretion.

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  1. Department of Anatomy, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, Canada

    D. V. Naik

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This study was supported by MRC Canada Grant no. MA-5160

The author wishes to thank Drs. Morry Givner and Michael Hirsch of Ayerst Research Laboratories, Montreal, for providing the synthetic LH-RH decapeptide (AY-24,031) and its anti-serum, and Mrs. Shaila Naik for her assistance in the preparation of this manuscript

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Naik, D.V. Immuno-histochemical localization of LH-RH during different phases of estrus cycle of rat, with reference to the preoptic and arcuate neurons, and the ependymal cells. Cell Tissue Res. 173, 143–166 (1976). https://doi.org/10.1007/BF00221371

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