Abstract
The urinary bladder depends on intracellular ATP for the support of a number of essential intracellular processes including contraction. The concentration of ATP is maintained constant primarily via the rapid transfer of a phosphate from creatine phosphate (CP) to ADP catalyzed by the enzyme creatine kinase (CK). Since muscular pathologies associated with diabetes are in part related to intracellular alterations in metabolism, we have characterized the CK activity in both skeletal muscle and urinary bladder from control and streptozotocin-diabetic rats.
The following is a summary of the results: 1) Bladder tissue from control rats showed linear kinetics with a Vmax = 390 nmoles/mg protein/min, and a Km = 275 µM. 2) Urinary bladder tissue isolated from diabetic rats displayed biphasic kinetics with Vmax = 65 and 324 nmoles/mg protein/min, and Km's = 10 µM and 190 µM respectively. 3) Skeletal muscle isolated from control rats showed linear kinetics with an approximate Vmax of 800 nmoles/mg protein/min and a Km of 280 µM CP. 4) Homogenates of skeletal muscle from diabetic rats showed complex kinetics not separable into distict component forms. 5) The Km for ADP for both skeletal muscle and bladder was approximately 10 µM.
These studies demonstrate that whereas bladders isolated from both control and diabetic rats possess a low-affinity isomer(s) of CK with similar maximum enzymatic activity, there is a high affinity isomer present within the urinary bladder muscle of diabetic rats that is not present in bladder tissue isolated from control rats. Skeletal muscle isolated from both diabetic and control rats exhibited a maximal activity 2 to 3 times higher than that of the bladder.
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Levin RM, High J, Wein AJ: Metabolic and contractile effects of anoxia on the rabbit urinary bladder. J Urol 128: 194–196, 1982
Wendt IR, Gibbs CL: Energy expenditure of longitudinal smooth muscle of rabbit urinary bladder. Am J Physiol 252: C88-C96, 1987
Paul RJ: Smooth muscle energetics. Ann Rev Physiol 51: 331–349, 1989
Butler RM, Siegman MJ, Mooers SU, Davies RE: High energy phosphate utilization during force development and force maintenance in mammalian smooth muscle. In: R Casteels, T Godfraind, JC Ruegg (eds) Excitation contraction coupling in smooth muscle. Elsevier, Amsterdam, 1977, pp 463–469
Koons S, Cooke R: Function of creatine kinase localization in muscle contraction. Adv Exp Med Biol 194: 129–137, 1986
Bessman SP: The creatine-creatine phosphate energy shuttle. Ann Rev Biochem 54: 831–862, 1985
Longhurst PA, Belis JA: Abnormalities of rat bladder contractility in streptozotocin-induced diabetes mellitus. J Pharmacol Exp Ther 238: 773–777, 1986
Moss HE, Lincoln J, Burnstock G: A study of bladder dysfunction during streptozotocin-induced diabetes in the rat using an in vitro whole bladder preparation. J Urol 138: 1279–1284, 1987
Andersson PO, Malmgren A, Uvelius B: Cystometrical and in vitro evaluation of urinary bladder function in rats with streptozotocin-induced diabetes. J Urol 139: 1359–1362, 1988
Van Lancker JL: Diabetes of pancreatic origin, the mode of action of insulin, and hypoglycemia. In: Molecular and Cellular Mechanisms in Disease, Springer-Verlag, N.Y. (1976) pp 495–527
Bell RH Jr, Hye RJ: Animal models of diabetes mellitus: Physiology and pathology. J Surg Res 35: 433–460, 1983
Boehringer Mannheim (1975): Biochemica Information II, Creatine Kinase, pp 58–59. Published by Boehringer Mannheim, W. Germany
Wein AJ, Raezer DM: Physiology of micturition. In: R Krane, Siroky M (eds) Clinical Neurourology, Boston, Little Brown & Co, 1979
Wein AJ, Levin RM, Barrett DM: Voiding Function: Relevant Anatomy, Physiology, and Pharmacology. In: JY Gillenwater, JT Grayhack, SS Howards, JD Duckett (eds) Adult and Pediatric Urology. Year Book Medical Publishers, Chicago, 1987
Paul RJ, Strauss JD, Krisanda JM: The effects of calcium on smooth muscle mechanics and energetics. In Siegman, Somlyo, Stephens (ed) Regulation and Contraction of Smooth Muscle, Alan Liss, N.Y., 1986, pp 319–332
Levin RM, Hypolite J, Ruggieri MR, Longhurst PA, Wein AJ: Effects of muscarinic stimulation on intracellular calcium in the rabbit bladder: Comparison with metabolic response. Pharmacol 39: 69–77, 1989
Seraydarian MW, Yamada T: Isozymes of creatine kinase in mammalian myocardial cell culture. Adv Exp Med Biol 194: 41–53, 1986
Shatton JB, Morris HP, Weinhouse S: Creatine kinase activity and isozyme composition in normal tissues and neoplasms of rats and mice. Cancer Res 39: 492–501, 1979
Kaye AM, Reiss NA, Weisman Y, Binderman I, Somjen D: Hormonal regulation of creatine kinase BB. Adv Exp Med Biol 194: 83–101, 1986
Longhurst PA, Wein AJ, Levin RM: In-Vivo urinary bladder function in rats following prolonged diabetic and nondiabetic diuresis. Neurourol Urodyn 9: 171–178, 1990
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Levin, R.M., Longhurst, P.A., Levin, S.S. et al. Creatine kinase activity of urinary bladder and skeletal muscle from control and streptozotocin-diabetic rats. Mol Cell Biochem 97, 153–159 (1990). https://doi.org/10.1007/BF00221057
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DOI: https://doi.org/10.1007/BF00221057