Abstract
Glucose disposal in skeletal muscle is a major target for insulin action and assessment of insulin-regulated glucose uptake under in vitro conditions allows the direct determination of insulin sensitivity in this organ. For this purpose, a variety of muscle preparations from different parts of the body can be used. We describe here a detailed protocol for using epitrochlearis muscle strips and additionally for using primary skeletal muscle cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jornayvaz FR, Samuel VT, Shulman GI (2010) The role of muscle insulin resistance in the pathogenesis of atherogenic dyslipidemia and nonalcoholic fatty liver disease associated with the metabolic syndrome. Annu Rev Nutr 30:273–90
DeFronzo RA, Tobin JD, Andres R (1979) Glucose clamp technique: a method for quantifying insulin secretion and resistance. Am J Physiol 237:E214–23
Eckardt K, Taube A, Eckel J (2011) Obesity-associated insulin resistance in skeletal muscle: Role of lipid accumulation and physical inactivity. Rev Endocr Metab Disord 12:163–72
Buren J, Lai YC, Lundgren M et al (2008) Insulin action and signalling in fat and muscle from dexamethasone-treated rats. Arch Biochem Biophys 474:91–101
Schultze SM, Jensen J, Hemmings BA et al (2011) Promiscuous affairs of PKB/AKT isoforms in metabolism. Arch Physiol Biochem 117:70–7
Zierath JR, Krook A, Wallberg-Henriksson H (2000) Insulin action and insulin resistance in human skeletal muscle. Diabetologia 43:821–35
Aslesen R, Jensen J (1998) Effects of epinephrine on glucose metabolism in contracting rat skeletal muscles. Am J Physiol 275:E448–56
Krebs AH, Henseleit K (1932) Untersuchungen über die Harnstoffbildung im Tierkörper. Hoppe-Seyler’s Z Physiol Chem 210:33–66
Wang Q, Khayat Z, Kishi K et al (1998) GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay. FEBS Lett 427:193–7
Jensen J, Jebens E, Brennesvik EO et al (2006) Muscle glycogen inharmoniously regulates glycogen synthase activity, glucose uptake, and proximal insulin signaling. Am J Physiol Endocrinol Metab 290:E154–E162
Wallberg-Henriksson H (1987) Glucose transport into skeletal muscle. Influence of contractile activity, insulin, catecholamines and diabetes mellitus. Acta Physiol Scand Suppl 564:1–80
Segal SS, Faulkner JA (1985) Temperature-dependent physiological stability of rat skeletal muscle in vitro. Am J Physiol 248:C265–70
Sell H, Laurencikiene J, Taube A et al (2009) Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes 58:2731–40
Acknowledgments
HS is supported by DFG SE 1922/2-1. JJ research has been supported by grants from Novo Nordisk Foundation. JE and JJ are supported by participation in COST BM0602 (Network supported by European Community). JE is supported by the Commission of the European Communities (collaborative project ADAPT, contract number HEALTH-F2-2008-201100).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Sell, H., Jensen, J., Eckel, J. (2012). Measurement of Insulin Sensitivity in Skeletal Muscle In Vitro. In: Joost, HG., Al-Hasani, H., Schürmann, A. (eds) Animal Models in Diabetes Research. Methods in Molecular Biology, vol 933. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-068-7_16
Download citation
DOI: https://doi.org/10.1007/978-1-62703-068-7_16
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-067-0
Online ISBN: 978-1-62703-068-7
eBook Packages: Springer Protocols