Abstract
Pepper (Capsicum chinense Jacq.) has been reported to be an important reservoir of resistance genes to tomato spotted wilt virus (TSWV). The genes for TSWV resistance present in three C. chinense lines (‘PI 152225’, ‘PI 159236’ and ‘Panca’) were investigated for allelism. All resistant lines were crossed with each other. Parents, F1, backcrosses and F2 populations (including reciprocals) developed from those crosses were mechanically inoculated with a highly virulent TSWV isolate. Susceptible C. annuum cv ‘Magda’ was used to check inoculum virulence. Fifty plants of the F1 hybrids; ‘Magda’ x ‘PI 152225’, ‘Magda’ x ‘PI 159236’, and ‘Magda’ x 'Panca, were also inoculated with the TSWV isolate. The resistance response in all C. chinense sources was associated with a localized, hypersensitive-like reaction that was phenotypically expressed as a prompt formation of large local lesions accompanied by premature leaf abscission. All F1 generations presented a final score of resistant; indicating that the expression of resistance to TSWV is conditioned by a dominant gene regardless of the source. The absence of segregation for resistance to TSWV that was observed in all generations of the crosses between C. chinense lines indicated that either a tightly linked group of genes exists or that the resistance is governed by the same single major gene (probably the already described Tsw gene). Previous reports have indicated that the Tsw gene is not effective against tospovirus members of serogroup II, i.e. tomato chlorotic spot virus (TCSV) and groundnut ring spot virus (GRSV). In the assay described here, all of the C. chinense lines showed, after mechanical inoculation, an identical susceptibility response to the TCSV and GRSV isolates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Black LL, Hobbs HA, Gatti JM Jr. (1991) Tomato spotted wilt virus resistance in Capsicum chinense PI 152225 and PI 159236. Plant Dis 75:853
Boiteux LS, de Avila AC (1993) Resistencia ao tomato spotted wilt virus (TSWV) na linhagen de pimenta CNPH 679 controlada por um gene dominate. Hortic Bras 11:63
Boiteux LS, de Avila AC (1994) Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense PI 159236. Euphytica 75:139–142
Boiteux LS, Giordano LB (1992) Screening Lycopersicon germplasm for resistance to a Brazilian isolate of tomato spotted wilt virus (TSWV). Tomato Genet Coop Rep 42:13–14
Boiteux LS, Giordano LB (1993) Genetic basis of resistance against two Tospovirus species in tomato (Lycopersicon esculentum). Euphytica 72:151–154
Boiteux LS, Nagata T (1993) Susceptibility of Capsicum chinense PI 159236 to tomato spotted wilt virus isolates in Brazil. Plant Dis 77:210
Boiteux LS, Cupertino FP, Reifschneider FJB (1993a) Capsicum chinense PI 159236: source of resistance to tomato spotted wilt virus and Phytophthora capsici. Capsicum Eggplant Newl 12:76
Boiteux LS, Nagata T, Dusi AN, de Avila AC (1993b) Natural occurrence of two Tospovirus species infecting Capsicum spp. in Brazil. Capsicum Eggplant Newsl 12:75
Boiteux LS, Nagata T, Dutra WP, Fonseca MEN (1993c) Sources of resistance to tomato spotted wilt virus (TSWV) in cultivated and wild species of Capsicum. Euphytica 67:89–94
Boiteux LS, Gonzaga V, Saturnino HM (1994) Groundnut ring spot tospovirus on field pepper (Capsicum annuum)in Janauba-MG. Fitopatol Bras 19:280
Bos L (1978) Symptoms of virus diseases in plants. Centre for Agricultural Publishing and Documentation, Wageningen, The Netherlands
Cupertino FP, Lin MT, Munoz JO (1984) Perdas na producao do pimentao induzidas pelo virus de vira-cabeca do tomateiro. Fitopatol Bras 9:397
Cupertino FP, Reifschneider, FJB, Batista MF (1988) Avaliacao de populacoes de Capsicum aos virus Y da batata e vira-cabeca do tomateiro. Fitopatol Bras 13:148
de Avila AC, de Haan P, Kormelink R, Resende RO, Goldbach R, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. J Gen Virol 74:153–159
Duval CM, Cupertino FP (1993) Preservacao da infectividade de isolados de Tospovirus. Fitopatol Bras 18:233–237
Fonseca MEN, Boiteux LS (1992) Resposta hipersensivel induzida pelo tomato spotted wilt virus em linhagens resistentes de Capsicum. Fitopatol Bras 17:201
Francki RIB, Fauquet CM, Knudson DD, Brown F (1991) Fifth report of the international comittee on taxonomy of viruses. Arch Virol [Suppl] 2:1–450
Fraser RSS (1990) The genetics of resistance to plant viruses. Annu Rev Phytopathol 28:179–200
Nagata T, Boiteux LS, Iizuka N, Dusi AN (1992) Hospedeiros diferenciais e serologia na identificacao de grupos de vira-cabeca do tomateiro em tomate e pimentao no Brasil. Fitopatol Bras 17:187
Nagata T, Boiteux LS, Iizuka N, Dusi AN (1993a) Identification of phenotypic variation of tospovirus isolates in Brazil based on serological analysis and differential host response. Fitopatol Bras 18:425–430
Nagata T, de Avila AC, de Melo PCT, Boiteux LS (1993b) Geographical distribution of Tospovirus species in Sao Paulo State and Federal District. Fitopatol Bras 18:319
Nelson RR (1973) Breeding plants for disease resistance — concepts and applications. Pennsylvania State University Press, Philadelphia, Penn.
Reifschneider FJB, Boiteux LS, Nagata, T (1992) Fonte de resistencia genetica multipla a Phytophthora capsici, tomato spotted wilt virus (TSWV) e potato Y (PVY) em Capsicum. Fitopatol Bras 17:209
White RF, Antoniw JF (1991) Virus-induced resistance responses in plants. Crit Rev Plant Sci 9:443–456
Author information
Authors and Affiliations
Additional information
Communicated by G. S. Khush
Rights and permissions
About this article
Cite this article
Boiteux, L.S. Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense . Theoret. Appl. Genetics 90, 146–149 (1995). https://doi.org/10.1007/BF00221009
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00221009