Summary
Photoperiod-sensitive rice (Oryza sativa L.) cultivars are widely grown in rainfed lowland areas with unfavorable water regimes. A molecular marker for the trait would be useful in genetic and physiological studies and in developing improved photoperiod-sensitive cultivars. Previous genetic studies identified a major gene for photoperiod sensitivity on chromosome 6. We have tested an isozyme marker and several RFLP probes mapping to chromosome 6 in an attempt to identify marker(s) tightly linked to photoperiod sensitivity in tropical rice cultivars. We report here that the isozyme gene Pgi-2 is linked (23.2±4.7 cM) to the photoperiod-sensitivity gene in the cultivar GEB-24. Although association of duration with Pgi-2 alleles can be used to detect segregation of the photoperiod sensitivity gene in crosses, it will probably not be useful as a marker in selection because of its loose linkage. In contrast, a gene for photoperiod sensitivity in the cultivar Puang Rai 2 was found to be closely linked to the rice genomic clone RG64. Among 15 F3 lines homozygous for photoperiod insensitivity, no recombinants were detected with RG64. This clone is thus an excellent probe to follow segregation of the major photoperiod-sensitivity gene in rice crosses.
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References
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278
Brar DS, de los Reyes BG, Panaud O, Sanchez A, Khush GS (1991) Genetic mapping in rice using isozyme and RFLP markers. In: Rice genetics II. Proc 2nd Int Rice Genet Symp, 14–18 May 1990, Int Rice Res Inst, Los Baños, Philippines, pp 137–145
Chang TT, Li CC, Vergara BS (1969) Component analysis from seeding to heading in rice by the basic vegetative phase and photoperiod sensitive phase. Euphytica 18:79–91
Glaszmann JC, de los Reyes BG, Khush GS (1988) Electrophoretic variation of isozymes in plumules of rice (Oryza sativa L.) —A key to identification of 76 alleles at 24 loci. IRRI Res. Paper Series No. 134. Int Rice Res Inst, Los Baños, Philippines
Kinoshita T (1986) Standardization of gene symbols and linkage maps in rice. In: Rice genetics. Proc Int Rice Genet Symp, 27–31 May 1985, Int Rice Res Inst, Los Baños, Philippines, pp 215–228
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Miah NM, Miah MAA, Dewan SBA (1986) Integrated breeding program for medium-deep, stagnant-water, rainfed rice in Bangladesh. In: Progress in rainfed lowland rice. Proc Int Rice Res Conf, 15–20 Oct 1984, Bhubaneswar, India, Int Rice Res Inst, Los Baños, Philippines, pp 217–222
Mohanty HK, Roy A, Das SR (1984) Varietal improvement in lowland rice. Oryza 21:72–87
Nanda JS, Coffman WR (1979) Alternative plant types and other agronomic characteristics for rainfed lowland rice. In: Rainfed lowland rice: Selected papers from the 1978 International Rice Research Conference. 17–21 Apr 1978, Los Baños, Philippines. Int Rice Res Inst, Los Baños, Philippines, pp 123–133
Nwe KT, Mackill DJ (1986) Photoperiod sensitivity in three rice crosses. Euphytica 35:751–760
Oosumi T, Miyazaki A, Uchimiya H, Kikuchi F, Yokoo M (1989) Analysis of glucose phosphate isomerase in near-isogenic lines, and cultivars of rice (Oryza sativa L.). Bot Mag Tokyo 102:283–289
Poonyarit M, Mackill DJ, Vergara BS (1989) Genetics of photoperiod sensitivity and critical daylength in rice. Crop Sci 29:647–652
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7:257–264
Tong Z, Wang T, Xu Y (1990) Evidence for involvement of phytochrome regulation in male sterility of a mutant of Oryza sauva L. Photochem Photobiol 52:161–164
Vergara BS, Jackson B, De Datta SK (1976) Deep water rice and its response to deep water stress. In: Climate and rice. Proc Symp, 24–27 Sep 1974. Int Rice Res Inst, Los Baños, Philippines, pp 301–319
Yokoo M, Fujimaki H (1971) Tight linkage of blast-resistance with late maturity observed in different indica varieties of rice. Jpn J Breed 21:35–39
Yokoo M, Kikuchi F, Nakane A, Fujimaki H (1980) Genetic analysis of heading date by aid of close linkage with blast resistance in rice. Bull Nat Inst Agric Sci Ser D, 31:95–126
Yu ZH, Mackill DJ, Bonman JM, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476
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Communicated by G. E. Hart
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Mackill, D.J., Salam, M.A., Wang, Z.Y. et al. A major photoperiod-sensitivity gene tagged with RFLP and isozyme markers in rice. Theoret. Appl. Genetics 85, 536–540 (1993). https://doi.org/10.1007/BF00220910
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DOI: https://doi.org/10.1007/BF00220910