Summary
Proteins from haploid megagametophytes from 18 trees were studied by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). A total of 222 seeds, an average of 12 per tree, were analysed individually. 150 protein spots appeared to segregate on the polyacrylamide gels in at least tree. Genetic interpretations were made to define the number of loci responsible for the presence versus absence, staining differences or position variation of the segregating spots. The complete covariation observed between some spots could be the result of either the separation of a single gene product into two or more constituents, very close linkage, or the action of a pleiotropic gene. Human genetics techniques were used to map the 84 putative loci detected. Sixty-five loci were organised in 17 linkage groups, whereas 19 remained unlinked.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams WT, Neale DB, Doerksen AH, Smith DB (1990) Inheritance and linkage of isozyme variants from seed and vegetative bud tissues in coastal Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb) Franco). Silvae Genet 39:3–4
Allona I, Casado R, Aragoncillo C (1992) Seed storage proteins from Pinus Pinaster Ait.: Homology of major components with 11S proteins from angiosperms (in press)
Anderson NG, Tollaksen SL, Pascoe FH, Anderson L (1985) Two-dimensional electrophoresis analysis of wheat seed proteins. Crop Sci 25:667–674
Bahrman N, Damerval C (1989) Linkage relationships of loci controlling protein amounts in maritime pine (Pinus pinaster Ait.). Heredity 63:267–274
Belanger FC, Kriz AL (1991) Molecular basis for allelic polymorphism of the maize globulin-1 gene. Genetics 129:863–872
Carlson JE, Tulsieram LK, Glaubitz JC, Luk VWK, Kauffeldt C, Rutlegde R (1991) Segregation of random amplified DNA markers in F1 progeny of conifers. Theor Appl Genet 83:194–200
Chang C, Bowman JL, Dejohn AW, Lander ES, Meyerowitz EM (1988) Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci USA 85:6856–6860
Collada C, Caballero RG, Casado R, Salcedo G, Aragoncillo C (1991) Subunit structure of legumin-like globulins of Fagus sylvatica seeds. J Exp Bot 42:1305–1310
Damerval C, le Guilloux M, Blaisonneau J, de Vienne D (1987a) A simplification of Heukeshoven and Dernick's silver staining of proteins. Electrophoresis 8:158–159
Damerval C, Hebert Y, de Vienne D (1987b) Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize. Theor Appl Genet 74:194–202
Donis-Keller H et al. (1987) A genetic linkage map of the human genome. Cell 51:319–337
El-Kassaby YA (1982) Association between allozyme genotypes and quantitative traits in Douglas-fir. Genetics 101:103–115
Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homologous tomato genome. Theor Appl Genet 83:49–57
Geburek T, Wang Q (1990) Inheritance of isozyme variants and their linkage relationships in Chinese fir (Cunninghamia lanceolata Hook). Euphytica 49:193–201
Goldman D, Rathna Giri R, O'Brien SJ (1987) A molecular phylogeny of the hominoid primates as indicated by two-dimensional protein electrophoresis. Proc Natl Acad Sci USA 84:3307–3311
Gottlieb LD, de Vienne D (1988) Assessment of pleiotropic effects of a gene substitution in pea by two-dimensional polyacrilamide gel electrophoresis. Genetics 119:705–710
Granier F, de Vienne D (1986) Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets. Anal Biochem 155:45–50
Heidecker G, Messing J (1986) Structural analysis of plant genes. Annu Rev Plant Physiol 37:439–466
Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphism. Theor Appl Genet 72:761–769
Higgins TJV (1984) Synthesis and regulation of major proteins in seeds. Annu Rev Plant Physiol 35:191–221
Hull GA, Halford NG, Kreis M, Shewry PR (1991) Isolation and characterization of genes encoding rye prolamins containing a highly repetitive sequence motif. Plant Mol Biol 17:1111–1115
Lander ES, Green P (1987) Construction of multilocus genetic linkage maps in humans. Proc Natl Acad Sci USA 84:2363–2367
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) Mapmaker: an interactive computer package of constructing primary genetic linkage maps for experimental and natural populations. Genomics 1:174–181
Landry BS, Hubert N, Etoh T, Harada JJ, Lincoln SE (1991) A genetic map for Brassica napus based on restriction fragment polymorphisms detected with expressed DNA sequences. Genome 34:543–552
Ledig FT, Guries RP, Bonefeld BA (1983) The relation of growth of heterozygosity in pitch pine. Evolution 37:1227–1238
Leonardi A, Damerval C, de Vienne D (1987) Inheritance of protein amounts: comparison of two-dimensional electrophoresis patterns of leaf sheats of two maize lines (Zea mays L.) and their hybrids. Genet Res 50:1–5
Leonardi A, Damerval C, de Vienne D (1988) Organ-specific variability and inheritance of maize proteins revealed by two-dimensional electrophoresis. Genet Res 52:97–103
Leonardi A, Damerval C, Herbert Y, Gallais A, de Vienne D (1991) Association of protein amount polymorphism (PAP) among maize lines with performances of their hybrids. Theor Appl Genet 82:552–560
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Neale DB, Wiliams CG (1991) Restriction fragment length poly morphism mapping in conifers and applications to forest genetic and tree improvement. Can J For Res 21:545–554
Niebling CR, Johnson K, Gerhold HD (1987) Electrophoretic analysis of genetic linkage in scots pine (Pinus sylvestris L.). Biochem Genet 25:803–814
Polans NO, Weeden NF, Thompson WF (1985) Inheritance, origanisation, and mapping of rbcS and cab multigene families in pea. Proc Natl Acad Sci USA 82:5083–5087
Soller M, Beckmann JS (1983) Genetic polymorphism in varietal identification and genetic improvement. Theor Appl Genet 67:25–33
Strauss SH (1986) Heterosis at allozyme loci under inbreeding and crossbreeding in Pinus attenuata. Genetics 113:115–134
Szmidt AE, Muona O (1989) Linkage relationships of allozyme loci in Pinus sylvestris. Hereditas 11:91–97
Tanksley SD, Bernatzky R, Lapitan NL, Prince JP (1988) Conservation of gene repertoire but not gene order in pepper and tomato. Proc Natl Acad Sci USA 85:6419–6423
Thoraval D, Regnacq M, Neuville P, Boucherie H (1990) Functional analysis of the yeast genome: use of two-dimensional gel electrophoresis to detect randomly cloned DNA sequences. Curr Genet 18:281–286
Vedel F, Delseny M (1987) Repetitivity and variability of higher plant genomes. Plant Physiol Biochem 25(2):191–210
Xie CY, Dancik BP, Yeh FC (1991) Inheritance and linkage of isozymes in Thuja orientalis. J Hered 82:329–334
Zivy M, Thiellement H, de Vienne D, Hofmann J-P (1983) Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis. Theor Appl Genet 66:1–7
Author information
Authors and Affiliations
Additional information
Communicated by P.M.A. Tigerstedt
Rights and permissions
About this article
Cite this article
Gerber, S., Rodolphe, F., Bahrman, N. et al. Seed-protein variation in maritime pine (Pinus pinaster Ait.) revealed by two-dimensional electrophoresis: genetic determinism and construction of a linkage map. Theoret. Appl. Genetics 85, 521–528 (1993). https://doi.org/10.1007/BF00220908
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220908