Summary
The regeneration of the islets of Langerhans in the guinea pig was studied after intravenous injection of alloxan. A number of β cells in the islets was destroyed within 24 h after alloxan, but after 48 h there was a rapid proliferation of the surviving cells of the islets. This depended on the dosage of the drug as well as the timing. Electron microscopy of the islet at 48 h showed that the dividing cells had small electron dense granules and resembled a subtype of normal A cells, whose function is not yet known. There were also many agranular cells in the islet. These two groups of cells seen in the regenerating islet could be precursor cells, which could differentiate into β cells. There was no evidence for transformation of duct cells or acinar cells into islet cells. None of the guinea pigs became permanently diabetic. This was probably due to inadequate dosage which resulted in only partial degeneration of the β cells followed by regeneration and recovery. There was also some regeneration of the liver, kidney and the adrenal cortex following alloxan.
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References
Adams, D.J., Harrison, R.G.: The vascularization of the rat pancreas and the effect of ischaemia on the islets of Langerhans. J. Anat. (Lond.) 87, 257–267 (1953)
Bensley, R.R.: Studies on the pancreas of the guinea pig. Amer. J. Anat. 12, 297–388 (1911)
Boquist, L., Falkmer, S.: The significance of agranular and ciliated cells. In: The structure and metabolism of the pancreatic islets (Falkmer, Hellman and Tajedal, eds.) Oxford-New York-Toronto-Sydney-Braunschweig: Pergamon Press 1970
Brosky, G., Logothetopoulos, J.: Streptozotocin diabetes in the mouse and guinea pig. Diabetes 18, 606–611 (1969)
Brown, R.E., Madge, G.E.: Cystic fibrosis and nesidioblastosis. Arch. Path. 92, 53–57 (1971)
Brown, R.E., Still, W.J.S.: Nesidioblastosis and the Zollinger-Ellison syndrome. Amer. J. dig. Dis. 13, 656–663 (1968)
Bunnag, S.C., Warder, N.E., Bunnag, S.: Effect of Alloxan on the mouse pancreas during and after recovery from diabetes. Diabetes 16, 83–89 (1967)
Caramia, F., Munger, B.L., Lacy, P.E.: The ultrastructural basis for identification of cell types in the pancreatic islets. I. Guinea pig. Z. Zellforsch. 67, 533–546 (1965)
Gomori, G.: A rapid one step trichrome stain. Amer. J. clin. Path. 20, 661–664 (1950)
House, E.L.: A histological study of the pancreas, liver and kidney both during and after recovery from alloxan diabetes. Endocrinology 62, 189–200 (1958)
Hughes, H.: An experimental study of regeneration in the islets of Langerhans with reference to the theory of balance. Acta anat. (Basel) 27, 1–61 (1956)
Johnson, D.D.: Alloxan administration in the guinea pig. A study of the regenerative phase in the islands of Langerhans. Endocrinology 47, 393–398 (1950)
Kern, H., Logothetopoulos, J.: In: The structure and metabolism of the pancreatic islets (Falkmer, Hellman and Tajedal, eds.) Oxford-New York-Toronto-Sydney-Braunschweig: Pergamon Press 1970a
Kern, H., Logothetopoulos, J.: Steroid diabetes in the guinea pig. Studies on the islet cell ultrastructure and regeneration. Diabetes 19, 145–154 (1970b)
Korcakova, L.: Mitotic division and its significance for regeneration of granulated B-cells in the islets of Langerhans in Alloxan diabetic rats. Folia morph. (Warszawa) 19, 25–31 (1971)
Logothetopoulos, J., Bell, E.A.: Histological and autoradiographic studies of the islets of mice injected with insulin antibody. Diabetes 15, 205–211 (1966)
Palade, G.E.: Intracysternal granules in the exocrine cells of the pancreas. J. biophys. biochem. Cytol. 2, 417–421 (1956)
Rerup, C.C.: Drugs producing diabetes through damage of insulin secreting cells. Pharmacol. Rev. 22, 485–518 (1970)
Scott, H.R.: Rapid staining of beta cell granules in pancreatic islets. Stain Technol. 27, 267–268 (1952)
Setalo, G., Blatniczky, L., Vigh, S.: Development and growth of the islets of Langerhans through acino-insular transformation in regenerating rat pancreas. Acta biol. Acad. Sci. lung. 23, 309–325 (1972)
Trinder, P.: Determination of blood glucose using 4-amino phenazone as oxygen acceptor. J. clin. Path. 22, 246 (1969)
Warren, S., Le Compte, P.M., Legg, M.A.: The pathology of diabetes mellitus, 4th ed., p. 79. Philadelphia: Lea and Febiger 1966
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The author is grateful to Professor R. Barer for his guidance and for providing the facilities for this study. Thanks are also due to Mrs. D. Barraclough for technical assistance and to Mrs. M. Hollingsworth for assistance with the photographs
This work was financed by a grant from the Cystic Fibrosis Research Trust
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Jacob, S. Regeneration of the islets of Langerhans in the guinea pig. Cell Tissue Res. 181, 277–286 (1977). https://doi.org/10.1007/BF00219987
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DOI: https://doi.org/10.1007/BF00219987