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Cytochemical localization of acid phosphatase in regenerated and dark-adapted eyes of a snail, Helix aspersa

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Summary

The role of the Golgi apparatus and the Golgi-endoplasmic reticulum-lysosomal complex (GERL) in the formation of lysosomes in the photosensory cells of regenerating and dark-adapted eyes of the garden snail Helix aspersa was examined with the use of acid phosphatase as a marker enzyme. In newly regenerated eyes, lead reaction deposit was restricted to the cisternae of GERL, a few small vesicles and some large secondary lysosomes. Dark-adapted sensory cells, on the other hand, were characterized by a heavy reaction product in the hypertrophied Golgi cisternae and GERL. Primary lysosomes were packaged by GERL cisternae in both the regenerating and the degenerating dark-adapted eyes. In the latter, these lysosomes may have been produced also by the Golgi apparatus. No reaction product was found in the rough endoplasmic reticulum, microvilli, or large aggregations of 800 Å photic vesicles in either kind of eye. In this study the lytic activity in differentiating sensory cells was significantly lower than that in degenerating cells suggesting that the increase in lysosomal activity in the latter was due to the absence of light. The effects of long dark-adaptation appear to be: 1. decrease in the production of photic vesicles; 2. increase in the formation of lysosomes; and 3. breakdown of photic vesicles by lysosomal activity.

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Authors and Affiliations

  1. Department of Zoology, University of California, Berkeley, California, USA

    Jean L. Brandenburger

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  1. Jean L. Brandenburger
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Supported by a grant-in-aid to Richard M. Eakin from the U.S. Public Health Service (GM10292)

The author is grateful to Drs. Richard M. Eakin, Mary E. Bentfeld, and Barbara A. Nichols for their advice and critical reading of the manuscript, to Lionel Thomas for technical assistance and to Dr. Dorothy R. Pitelka for the use of the Siemens Elmiskop 102

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Brandenburger, J.L. Cytochemical localization of acid phosphatase in regenerated and dark-adapted eyes of a snail, Helix aspersa . Cell Tissue Res. 184, 301–313 (1977). https://doi.org/10.1007/BF00219892

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