Summary
The parenchyma of the subfornical organ (SFO) of the Japanese quail was studied by light and electron microscopy. The SFO consists of ependymal, intermediate, and basal (perimeningeal) layers. In the intermediate layer, neurons, glial cells, and their processes are found. Axons containing dense core granules approximately 80 nm in diameter are numerous, some of which make synaptic contact with the neuronal perikarya or dendrites. Synaptic vesicles in some axons contain a dense dot in the interior after treatment with 5-hydroxydopamine. The activity of the SFO, which is probably concerned with elicitation of drinking by angiotensin II, may be regulated at least partly by afferent monoaminergic axons. Capillaries with a non-fenestrated endothelium are occasionally found in the parenchyma. The basal layer is occupied by glial processes abutting on the digitating layer of perivascular connective tissue of meningeal vessels. The endothelium of these vessels is occasionally fenestrated. Trypan blue injected systemically accumulated in the SFO, but not in the deeper areas of the brain. The absence of a blood-brain barrier is suggested in the SFO.
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Abdelaal, A.E., Assaf, S.Y., Kucharczyk, J., Mogenson, G.J.: Effect of ablation of the subfornical organ on water intake elicited by systemically administered angiotensin-II. Canad. J. Physiol. Pharmacol. 52, 1217–1220 (1974)
Akert, K.: The mammalian subfornical organ. J. neuro-visc. Relat., Suppl. 9, 78–93 (1969)
Akert, K., Pfenninger, K., Sandri, C.: The fine structure of synapses in the subfornical organ of the cat. Z. Zellforsch. 81, 537–556 (1967)
Andres, K.H.: Der Feinbau des Subfornikalorgans vom Hund. Z. Zellforsch. 68, 445–473 (1965)
Dellmann, H.-D., Simpson, J.B.: Comparative ultrastructure and function of the subfornical organ. In: Brain-endocrine interaction II (K.M. Knigge, D.E. Scott, H. Kobayashi, S. Ishii, eds.), pp. 166–189. Basel: Karger 1975
Dellmann, H.-D., Simpson, J.B.: Regional differences in the morphology of the rat subfornical organ. Brain Res. 116, 389–400 (1976)
Dempsey, E.W.: Fine-structure of the rat's intercolumnar tubercle and its adjacent ependyma and choroid plexus, with especial reference to the appearance of its sinusoidal vessels in experimental argyria. Exp. Neurol. 22, 568–589 (1968)
Dierickx, K.: The subfornical organ, a specialized osmoreceptor. Naturwissenschaften 50, 163–164 (1963)
George, J.M.: Localization in hypothalamus of increased incorporation of 3H cytidine into RNA in response to oral hypertonic saline. Endocrinology 92, 1550–1555 (1973)
George, J.M.: Hypothalamic sites of incorporation of [3H]cytidine into RNA in response to oral hypertonic saline. Brain Res. 73, 184–187 (1974)
George, J.M., Penrose, M.: Increased incorporation of [3H]uridine into RNA in the brain subfornical organ of ovariectomized mice. Brain Res. 97, 167–170 (1975)
Ishii, S., Wada, M., Oota, Y.: Identification of neurosecretory granules carrying adenohypophysial hormone-releasing hormone. In: Brain-endocrine interaction II (K.M. Knigge, D.E. Scott, H. Kobayashi, S. Ishii, eds.), pp. 70–79. Basel: Karger 1975
Koella, W.P., Sutin, J.: Extra-blood-brain-barrier brain structures. Int. Rev. Neurobiol. 10, 31–55 (1967)
Kucharczyk, J., Assaf, S.Y., Mogenson, G.J.: Differential effects of brain lesions on thirst induced by the administration of angiotensin-II to the preoptic region, subfornical organ and anterior third ventricle. Brain Res. 108, 327–337 (1976)
Legait, H., Legait, E.: Les vois extra-hypophysaires des noyaux neurosécrétoires hypothalamiques chez les Batraciens et les Reptiles. Acta anat. (Basel) 30, 429–443 (1957)
Leonhardt, H., Lindemann, B.: Surface morphology of the subfornical organ in the rabbit's brain. Z. Zellforsch. 146, 243–260 (1973)
Mikami, S.: Ultrastructure of the organum vasculosum of the lamina terminalis of the Japanese quail, Coturnix coturnix japonica. Cell Tiss. Res. 172, 227–243 (1976)
Pfenninger, K., Akert, K., Sandri, C., Bruppacher, H.: Zum Feinbau des Subfornikalorgans der Katze. III. Nerven- und Gliazellen. Schweiz. Arch. Neurol. Neurochir. Psychiat. 100, 232–254 (1967)
Pines, L., Scheftel, M.: Ist bei den niederen Vertebraten ein Homologon des subfornikalen Organes der Säugetiere festzustellen? Anat. Anz. 67, 203–216 (1929)
Reichold, S.: Untersuchungen über die Morphologie des subfornikalen und des subcommissuralen Organes bei Säugetieren und Sauropsiden. Z. mikr.-anat. Forsch. 52, 455–479 (1942)
Richards, J.G., Tranzer, J.-P.: The ultrastructural localization of amine storage sites in the central nervous system with the aid of a specific marker, 5-hydroxydopamine. Brain Res. 17, 463–469 (1970)
Rohr, V.U.: Zum Feinbau des Subfornikalorgans der Katze. I. Der Gefäßapparat. Z. Zellforsch. 73, 246–271 (1966a)
Rohr, V.U.: Zum Feinbau des Subfornikalorgans der Katze. II. Neurosekretorische Aktivität. Z. Zellforsch. 75, 11–34 (1966b)
Rudert, H.: Das Subfornikalorgan und seine Beziehungen zu dem neurosekretorischen System im Zwischenhirn des Frosches. Z. Zellforsch. 65, 790–804 (1965)
Rudert, H., Schwink, A., Wetzstein, R.: Die Feinstruktur des Subfornikalorgans beim Kaninchen. I. Die Blutgefäße. Z. Zellforsch. 74, 252–270 (1966)
Rudert, H., Schwink, A., Wetzstein, R.: Die Feinstruktur des Subfornikalorgans beim Kaninchen. II. Das neuronale und gliale Gewebe. Z. Zellforsch. 88, 145–179 (1968)
Sarrat, R.: Enzymhistochemische Untersuchungen am Subfornikalorgan der Ratte. Experientia (Basel) 24, 1239–1240 (1968)
Schinko, L, Rohrschneider, L, Wetzstein, R.: Elektronenmikroskopische Untersuchungen am Subfornikalorgan der Maus. Z. Zellforsch. 123, 277–294 (1972)
Simpson, J.B., Routtenberg, A.: Subfornical organ: Site of drinking elicitation by angiotensin II. Science 181, 1172–1174 (1973)
Simpson, J.B., Routtenberg, A.: Subfornical organ: Acetylcholine application elicits drinking. Brain Res. 79, 157–164 (1974)
Simpson, J.B., Routtenberg, A.: Subfornical organ lesions reduce intravenous angiotensin-induced drinking. Brain Res. 88, 154–161(1975)
Stumpf, W.E.: Estrogen-neurons and estrogen-neuron systems in the periventricular brain. Amer. J. Anat. 129, 207–218 (1970)
Takei, Y.: The role of the subfornical organ in drinking induced by angiotensin in the Japanese quail, Coturnix coturnix japonica. Cell Tiss. Res. 185, 175–181 (1977)
Takei, Y., Tsuneki, K., Kobayashi, H.: Surface fine structure of the subfornical organ of the Japanese quail, Coturnix coturnix japonica. Cell Tiss. Res. (in press, 1978)
Takei, Y., Kobayashi, H., Yanagisawa, M., Bando, T.: Involvement of monoaminergic fibers in angiotensin-induced drinking in the Japanese quail, Coturnix coturnix japonica. (In preparation, 1979)
Wetzig, H., Palkovits, M.: Die Entwicklung des Organon subfornicale bei der Sturmmöve Larus canus L. Z. mikr.-anat. Forsch. 79, 283–291 (1968)
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Tsuneki, K., Takei, Y. & Kobayashi, H. Parenchymal fine structure of the subfornical organ in the Japanese quail, Coturnix coturnix japonica . Cell Tissue Res. 191, 405–419 (1978). https://doi.org/10.1007/BF00219805
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DOI: https://doi.org/10.1007/BF00219805