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Catecholaminergic Structures of the Rat Subfornical Organ

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Abstract

Due to the high importance of investigating the subfornical organ and its tissue components and mediator systems, the aim of this work was to study the morphological features of the catecholaminergic innervation of this area. Using the methods of immunohistochemistry and confocal microscopy, preparations of the rat subfornical organ were studied on postnatal days 14 and 30 and at the age of 4–6 months. The main direction of the ingrowth of catecholaminergic fibers into the subfornical organ at the early stages was determined. It was found that the processes of catecholaminergic cells can contact the cells covering the subfornical organ and can penetrate through the ependymal layer. This allows them to contact the cerebrospinal fluid directly and, presumably, affect its composition. It is shown that some of the fibers run in parallel to the basal processes of specialized ependymal cells, tanycytes, which suggests their possible function as a scaffold for growing catecholaminergic fibers in postnatal development. This is the first study to demonstrate the presence of intrinsic catecholaminergic neurons in the subfornical organ.

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Funding

The reported study was funded by RSF according to the research project № 22-25-00105, https://rscf.ru/project/ 22-25-00105/

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  1. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    V. A. Razenkova & D. E. Korzhevskii

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  1. V. A. Razenkova
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  2. D. E. Korzhevskii
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Correspondence to V. A. Razenkova.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. All procedures involving animals were carried out in accordance with the guidelines established by the Directive 86/609/EEC on the protection of Animals used for Experimental and other scientific purposes (Strasbourg, 1986) and the Rules of Good Laboratory Practice (order no. 199n of the Russian Ministry of Health dated April 1, 2016). The study was approved by the Local Ethics Committee of the Institute of Experimental Medicine, conclusion no. 1/22 dated February 18, 2022.

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Translated by M. Batrukova

Abbreviations: BBB—blood–brain barrier; SFO—subfornical organ; TH—tyrosine hydroxylase; CSF—cerebrospinal fluid.

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Razenkova, V.A., Korzhevskii, D.E. Catecholaminergic Structures of the Rat Subfornical Organ. Cell Tiss. Biol. 16, 568–575 (2022). https://doi.org/10.1134/S1990519X22060062

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