Summary
Tannic acid in glutaraldehyde was used to stain microtubules in myelinated and unmyelinated axons of rat sciatic nerve. In the majority of areas the tannic acid failed to penetrate the unmyelinated axons whilst penetrating neighbouring myelinated axons, suggesting a difference in the ability of the two types of nerves to exclude tannic acid. Where tannic acid had penetrated the unmyelinated axons the 13 protofilament substructure and size of the microtubules appeared identical to those seen in the myelinated axons.
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Futaesaku, Y., Mizuhira, V., Nakamura, H.: A new fixation method using tannic acid for electron microscopy and some observations of biological specimens. Proc. Int. Congr. Histochem. and Cytochem. 4, 155–158 (1972)
Livingston, A.: Microtubules in neurosecretory neurones of the posterior pituitary of the rat. Cell Tiss. Res. 180, 253–261 (1977)
Livingston, A., Wilks, P.: Perivascular regions of the rat neural lobe. Cell Tiss. Res. 174, 273–280 (1976)
Livingston, A., Wood, J.: Microtubules in myelinated and unmyelinated axons from rat sciatic nerve. J. Physiol. (Lond.) 263, 216–217P (1976)
Livingston, A., Wood, J.: Effect of depolymerizing stimuli on microtubules of neurosecretory neurones. J. Endocr. 72, 61–62P (1977)
Tilney, L.G., Bryan, J., Bush, D.J., Fujiwara, K., Mooseker, M.S., Murphy, D.B., Snyder, D.H.: Microtubules, evidence for 13 protofilaments. J. Cell Biol. 59, 267–275 (1973)
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Livingston, A. Microtubules in myelinated and unmyelinated axons of rat sciatic nerve. Cell Tissue Res. 182, 401–407 (1977). https://doi.org/10.1007/BF00219774
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DOI: https://doi.org/10.1007/BF00219774