Skip to main content
Log in

The autonomic innervation of rabbit salivary glands studied electron microscopically after 5-hydroxydopamine administration

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

In submandibular glands of rabbits both adrenergic and cholinergic axons are intimately associated with parenchymal cells of the intercalary ducts and the granular tubules, lying beneath the basement membrane and often in the space between the parenchymal cell and an associated myoepithelial cell. The submandibular acini receive a less intimate and less plentiful innervation by adrenergic and cholinergic axons which remain outside the basement membrane and are still associated with Schwann cells. Occasional axons of both adrenergic and cholinergic type occur beneath the basement membrane of submandibular striated ducts in intimate association with basal parts of the cells.

In the parotid glands numerous adrenergic and cholinergic axons are found beneath the basement membrane of acini and intercalary ducts in intimate association with the cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arnstein, C: Zur Morphologie der sekretorischen Nervenapparate. Anat. Anz. 10, 410–419 (1895)

    Google Scholar 

  • Bloom, G.D., Carlsöö, B., Gustafson, H.: An electron microscopic study on the autonomic innervation of the rabbit parotid gland. Experientia (Basel) 32, 1190–1191 (1976)

    Google Scholar 

  • Castle, J.D., Jamieson, J.D., Palade, G.E.: Radioautographic analysis of the secretory process in the parotid acinar cell of the rabbit. J. Cell Biol. 53, 290–311 (1972)

    Google Scholar 

  • Chiba, T.: Electron microscopic and histochemical studies on the synaptic vesicles in mouse vas deferens and atrium after 5-hydroxydopamine administration. Anat. Rec. 176, 35–48 (1973)

    Google Scholar 

  • Cope, G.H., Williams, M.A.: Exocrine secretion in the parotid gland: A stereological analysis at the electron microscopic level of the zymogen granule content before and after isoprenaline induced secretion. J. Anat. (Lond.) 116, 269–284 (1973)

    Google Scholar 

  • Ehinger, B., Garrett, J.R., Ohlin, P.: Adrenergic nerves in the major salivary glands of the rabbit. Experientia (Basel) 23, 924 (1967)

    Google Scholar 

  • Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence. J. roy. micr. Soc. 87, 259–276 (1967)

    Google Scholar 

  • Freitag, P., Engel, M.B.: Autonomic innervation in rabbit salivary glands. Anat. Rec. 167, 87–106 (1970)

    Google Scholar 

  • Garrett, J.R.: The innervation of salivary glands. I. Cholinesterase positive nerves in normal glands of the cat. J. roy. micr. Soc. 85, 135–148 (1966)

    Google Scholar 

  • Garrett, J.R.: Neuro-effector sites in salivary glands. In: Oral physiology, pp. 83–97, eds. N. Emmelin and Y. Zotterman. Oxford: Pergamon 1972

    Google Scholar 

  • Garrett, J.R.: Innervation of salivary glands, morphological considerations. In: Secretory mechanisms of exocrine glands, pp. 17–27, eds. N.A. Thorn and O.H. Petersen. Benzon Symposium VII, Copenhagen: Munksgaard 1974

    Google Scholar 

  • Garrett, J.R., Kemplay, S.K.: The adrenergic innervation of the submandibular gland of the cat, and the effects of various surgical denervations on these nerves. A histochemical and ultrastructural study including the use of 5-hydroxydopamine. J. Anat. (Lond.) (in press)

  • Garrett, J.R., Thulin, A.: Changes in parotid acinar cells accompanying salivary secretion in rats on sympathetic or parasympathetic nerve stimulation. Cell Tiss. Res. 159, 179–193 (1975)

    Google Scholar 

  • Gomori, G.: Microscopic histochemistry. University of Chicago Press 1952

  • Hand, A.R.: Adrenergic and cholinergic nerve terminals in the rat parotid gland. Electron microscopic observations on permanganate-fixed glands. Anat. Rec. 173, 131–140 (1972)

    Google Scholar 

  • Harrop, T.J., Garrett, J.R.: Effects of preganglionic sympathectomy on secretory changes in parotid acinar cells of rats on eating. Cell Tiss. Res. 154, 135–150 (1974)

    Google Scholar 

  • Harrop, T.J., Mackay, B.: Electron microscopic observations on myoepithelial cells and secretory nerves in rat salivary glands. J. Canad. dent. Ass. 34, 480–488 (1968)

    Google Scholar 

  • Kagayama, M., Nishiyama, A.: Comparative aspect on the innervation of submandibular glands in cat and rabbit; an electron microscopic study. Tohoku J. exp. Med. 108, 179–193 (1972)

    Google Scholar 

  • Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A-138A (1965)

    Google Scholar 

  • Norberg, K.-A., Olson, L.: Adrenergic innervation of salivary glands in the rat. Z. Zellforsch. 68, 183–189 (1965)

    Google Scholar 

  • Nordenfelt, I, Ohlin, P.: Supersensitivity of salivary glands of rabbits. Acta physiol. scand. 41, 12–17 (1957)

    Google Scholar 

  • Rodriguez, E.M.: Fixation of the central nervous system by perfusion of the cerebral ventricles with a threefold aldehyde mixture. Brain Res. 15, 395–412 (1970)

    Google Scholar 

  • Schneyer, L.H., Young, J.A., Schneyer, C.A.: Salivary secretion of electrolytes. Physiol. Revs. 52, 720–777 (1972)

    Google Scholar 

  • Smaje, L.H.: Spontaneous salivation in the rabbit submandibular gland. J. Physiol. (Lond.) 231, 179–193 (1973)

    Google Scholar 

  • Thulin, A., Garrett, J.R.: Secretory and structural effects of 6-hydroxydopamine on normal parotid glands of rats, and at different times after surgical sympathectomy. Quart. J. exp. Physiol. 61, 15–21 (1976)

    Google Scholar 

  • Tranzer, J.P., Thoenen, H.: Electronmicroscopic localization of 5-hydroxydopamine (3,4,5-trihydroxy-phenyl-ethylamine), a new “false” sympathetic transmitter. Experientia (Basel) 23, 743 (1967)

    Google Scholar 

  • Yamamoto, I., Inoki, R., Tsujimoto, A., Kojima, S.: The role of the sympathetic nervous system in amylase secretion elicited by parasympathetic nerve stimulation. Europ. J. Pharmacol. 3, 117–122 (1968)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This work has been helped by the technical assistance of Mr. P.S.A. Rowley

Rights and permissions

Reprints and permissions

About this article

Cite this article

Garrett, J.R. The autonomic innervation of rabbit salivary glands studied electron microscopically after 5-hydroxydopamine administration. Cell Tissue Res. 178, 551–562 (1977). https://doi.org/10.1007/BF00219574

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00219574

Key words

Navigation