Summary
In submandibular glands of rabbits both adrenergic and cholinergic axons are intimately associated with parenchymal cells of the intercalary ducts and the granular tubules, lying beneath the basement membrane and often in the space between the parenchymal cell and an associated myoepithelial cell. The submandibular acini receive a less intimate and less plentiful innervation by adrenergic and cholinergic axons which remain outside the basement membrane and are still associated with Schwann cells. Occasional axons of both adrenergic and cholinergic type occur beneath the basement membrane of submandibular striated ducts in intimate association with basal parts of the cells.
In the parotid glands numerous adrenergic and cholinergic axons are found beneath the basement membrane of acini and intercalary ducts in intimate association with the cells.
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References
Arnstein, C: Zur Morphologie der sekretorischen Nervenapparate. Anat. Anz. 10, 410–419 (1895)
Bloom, G.D., Carlsöö, B., Gustafson, H.: An electron microscopic study on the autonomic innervation of the rabbit parotid gland. Experientia (Basel) 32, 1190–1191 (1976)
Castle, J.D., Jamieson, J.D., Palade, G.E.: Radioautographic analysis of the secretory process in the parotid acinar cell of the rabbit. J. Cell Biol. 53, 290–311 (1972)
Chiba, T.: Electron microscopic and histochemical studies on the synaptic vesicles in mouse vas deferens and atrium after 5-hydroxydopamine administration. Anat. Rec. 176, 35–48 (1973)
Cope, G.H., Williams, M.A.: Exocrine secretion in the parotid gland: A stereological analysis at the electron microscopic level of the zymogen granule content before and after isoprenaline induced secretion. J. Anat. (Lond.) 116, 269–284 (1973)
Ehinger, B., Garrett, J.R., Ohlin, P.: Adrenergic nerves in the major salivary glands of the rabbit. Experientia (Basel) 23, 924 (1967)
Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence. J. roy. micr. Soc. 87, 259–276 (1967)
Freitag, P., Engel, M.B.: Autonomic innervation in rabbit salivary glands. Anat. Rec. 167, 87–106 (1970)
Garrett, J.R.: The innervation of salivary glands. I. Cholinesterase positive nerves in normal glands of the cat. J. roy. micr. Soc. 85, 135–148 (1966)
Garrett, J.R.: Neuro-effector sites in salivary glands. In: Oral physiology, pp. 83–97, eds. N. Emmelin and Y. Zotterman. Oxford: Pergamon 1972
Garrett, J.R.: Innervation of salivary glands, morphological considerations. In: Secretory mechanisms of exocrine glands, pp. 17–27, eds. N.A. Thorn and O.H. Petersen. Benzon Symposium VII, Copenhagen: Munksgaard 1974
Garrett, J.R., Kemplay, S.K.: The adrenergic innervation of the submandibular gland of the cat, and the effects of various surgical denervations on these nerves. A histochemical and ultrastructural study including the use of 5-hydroxydopamine. J. Anat. (Lond.) (in press)
Garrett, J.R., Thulin, A.: Changes in parotid acinar cells accompanying salivary secretion in rats on sympathetic or parasympathetic nerve stimulation. Cell Tiss. Res. 159, 179–193 (1975)
Gomori, G.: Microscopic histochemistry. University of Chicago Press 1952
Hand, A.R.: Adrenergic and cholinergic nerve terminals in the rat parotid gland. Electron microscopic observations on permanganate-fixed glands. Anat. Rec. 173, 131–140 (1972)
Harrop, T.J., Garrett, J.R.: Effects of preganglionic sympathectomy on secretory changes in parotid acinar cells of rats on eating. Cell Tiss. Res. 154, 135–150 (1974)
Harrop, T.J., Mackay, B.: Electron microscopic observations on myoepithelial cells and secretory nerves in rat salivary glands. J. Canad. dent. Ass. 34, 480–488 (1968)
Kagayama, M., Nishiyama, A.: Comparative aspect on the innervation of submandibular glands in cat and rabbit; an electron microscopic study. Tohoku J. exp. Med. 108, 179–193 (1972)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A-138A (1965)
Norberg, K.-A., Olson, L.: Adrenergic innervation of salivary glands in the rat. Z. Zellforsch. 68, 183–189 (1965)
Nordenfelt, I, Ohlin, P.: Supersensitivity of salivary glands of rabbits. Acta physiol. scand. 41, 12–17 (1957)
Rodriguez, E.M.: Fixation of the central nervous system by perfusion of the cerebral ventricles with a threefold aldehyde mixture. Brain Res. 15, 395–412 (1970)
Schneyer, L.H., Young, J.A., Schneyer, C.A.: Salivary secretion of electrolytes. Physiol. Revs. 52, 720–777 (1972)
Smaje, L.H.: Spontaneous salivation in the rabbit submandibular gland. J. Physiol. (Lond.) 231, 179–193 (1973)
Thulin, A., Garrett, J.R.: Secretory and structural effects of 6-hydroxydopamine on normal parotid glands of rats, and at different times after surgical sympathectomy. Quart. J. exp. Physiol. 61, 15–21 (1976)
Tranzer, J.P., Thoenen, H.: Electronmicroscopic localization of 5-hydroxydopamine (3,4,5-trihydroxy-phenyl-ethylamine), a new “false” sympathetic transmitter. Experientia (Basel) 23, 743 (1967)
Yamamoto, I., Inoki, R., Tsujimoto, A., Kojima, S.: The role of the sympathetic nervous system in amylase secretion elicited by parasympathetic nerve stimulation. Europ. J. Pharmacol. 3, 117–122 (1968)
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This work has been helped by the technical assistance of Mr. P.S.A. Rowley
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Garrett, J.R. The autonomic innervation of rabbit salivary glands studied electron microscopically after 5-hydroxydopamine administration. Cell Tissue Res. 178, 551–562 (1977). https://doi.org/10.1007/BF00219574
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DOI: https://doi.org/10.1007/BF00219574