Summary
The hormone-induced pigment dispersion in primary cultures of xanthophores of goldfish (Carassius auratus L.) has been shown to involve the dispersion of not only carotenoid droplets but also of smooth endoplasmic reticulum. The dispersion of these organelles is inhibited by cytochalasin B and is accompanied by thinning of the cell body, thickening of the processes, and also overall changes in cellular morphology (process extension) under certain conditions. Electron microscopic examination of heavy meromyosin treated glycerinated xanthophores in scales revealed the presence of actin filaments in these cells.
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Bagnara, J.T., Hadley, M.E.: Chromatophores and color change. Englewood Cliffs, New Jersey: Prentice-Hall Inc. 1973
Bikle, D., Tilney, L.G., Porter, K.R.: Microtubules and pigment migration in the melanophore of Fundulus heteroditus. Protoplasma 61, 322–345 (1966)
Bloom, W., Fawcett, D.W.: A textbook of histology, 10th ed. Philadelphia: Saunders 1975
Butman, B., Obika, M., Tchen, T.T.,' Taylor, J.D.: Two types of amphibian iridophores and their response to α-MSH. Amer. Zool. 17, 911 (1977)
Byers, H.R., Porter, K.R.: Transformation in the structure of the cytoplasmic ground substance in erythrophores during pigment aggregation and dispersion. I. A study using whole-cell preparations in stereo high voltage electron microscopy. J. Cell Biol. 75, 541–558 (1977)
Fujii, R., Novales, R.R.: Cellular aspects of the control of physiological color changes in fishes. Amer. Zool. 9, 453–463 (1969)
Green, I.: Mechanism of movements of granules in melanocytes of Fundulus heteroditus. Proc. nat. Acad. Sci. (Wash.) 599, 1179–1186 (1968)
Ishikawa, H., Bischoff, R., Holtzer, H.: Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J. Cell Biol. 43, 312–328 (1969)
Jimbow, K., Davison, P.F., Pathak, M.A., Fitzpatrick, T.B.: Cytoplasmic filaments in melanocytes. In: Pigment cell (V. Riley, ed.), Vol. 3, pp. 13–32. Basel: S. Karger 1976
Lazarides, E.: Two general classes of cytoplasmic actin filaments in tissue culture cells: The role of tropomyosin. J. Supramolec. Struc. 5, 531–563 (1976)
Malawista, S.E.: Cytochalasin B reversibly inhibits melanin granule movement in melanocytes. Nature (Lond.) 2b34, 354–355 (1971)
McGuire, J., Moellmann, G.: Cytochalasin B: Effects on microfilaments and movement of melanin granules within melanocytes. Science 175, 642–644 (1972)
McGuire, J., Moellmann, G., McKeon, F.: Cytochalasin B and pigment granule translocation. J. Cell Biol. 52, 754–758 (1972)
Murphy, D.B.: The mechanism of microtubule-dependent movement of pigment granules in teleost Chromatophores. Ann. N.Y. Acad. Sci. 253, 692–701 (1975)
Murphy, D.B., Tilney, L.G.: The role of microtubules in the movement of pigment granules in teleost melanophores. J. Cell Biol. 61, 757–779 (1974)
Murray, R.L., Dubin, M.W.: The occurrence of actinlike filaments in association with migrating pigment granules in frog retinal pigment epithelium. J. Cell Biol. 64, 705–710 (1975)
Novales, R.R., Novales, B.J.: Effect of Cytochalasin B on the response of the Chromatophores of isolated frog skin to MSH, theophylline, and dibutyryl cyclic AMP. Gen comp. Endocr. 19, 363–366 (1972)
Obika, M.: The changes in cell shape during pigment migration in melanophores of a teleost, Oryzias latipes. J. exp. Zool. 191, 427–432 (1975)
Ozato, K.: Effects of ACTH, adenyl compounds, and methylxanthines on goldfish erythrophores in culture. Gen. comp. Endocr. 31, 335–342 (1977)
Pollard, T.D.: Cytoskeletal functions of cytoplasmic contractile proteins. J. Supramolec. Struc. 5, 317–334 (1976)
Pollard, T.D., Rifkin, D.: Modification of mammalian cell shape: Redistribution of intracellular actin by SV40 virus, proteases, cytochalasin B and dimethylsulfoxide. In: Cell motility. Book A, pp. 389–403.: Cold Spring Harbor Laboratory 1976
Pollard, T.D., Weihing, R.R.: Actin and myosin and cell movement. Crit. Rev. Biochem. 2, 1–65 (1974)
Porter, K.R.: Introduction: motility in cells. In: Cell motility. Book A, pp. 1–28: Cold Spring Harbor Laboratory 1976
Schliwa, M.: Microtubule distribution and melanosome movements in fish melanophores. In: Microtubules and microtubules inhibitors (M. Borgers and M. de Brabander, eds.). pp. 215–228. Amsterdam: North-Holland Publishing Co. 1975
Schliwa, M., Bereiter-Hahn, J.: Pigment movements in fish melanophores: Morphological and physiological studies. II. Cell shape and microtubules. Z. Zellforsch. 147, 107–125 (1973a)
Schliwa, M., Bereiter-Hahn, J.: Pigment movements in fish melanophores: Morphological and physiological studies. III. The effects of colchicine and vinblastine. Z. Zellforsch. 147, 127–148 (1973b)
Schliwa, M., Bereiter-Hahn, J.: Pigment movements in fish melanophores: Morphological and physiological studies. V. Evidence of a microtubule-independent contractile system. Cell. Tiss. Res. 158, 61–73 (1975)
Szent-Györgyi, A.G.: Meromyosins, the subunits of myosin. Arch. Biochem. Biophys. 42, 305–320 (1953)
Taylor, J.D., Bagnara, J.T.: Dermal chromatophores. Amer. Zool. 12, 43–62 (1972)
Turner, C.W., Bagnara, J.T.: General endocrinology, 6th ed. Philadelphia: Saunders 1976
Wikswo, M., Novales, R.R.: The effect of colchicine on microtubules in the melanophores of Fundulus heteroclitus. J. Ultrastruct. Res. 41, 189–201 (1972)
Winchester, J.D., Ngo, F., Tchen, T.T., Taylor, J.D.: Hormone-induced dispersion or aggregation of carotenoid-containing smooth endoplasmic reticulum in cultured xanthophores from the goldfish, Carassius auratus L. Endocrin. Res. Comm. 3, 335–342 (1976)
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This work was supported, in part, by grants AM-5384 and AM-13724 from U.S.P.H.S.
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Obika, M., Lo, S.J., Tchen, T.T. et al. Ultrastructural demonstration of hormone-induced movement of carotenoid droplets and endoplasmic reticulum in xanthophores of the goldfish, Carassius auratus L.. Cell Tissue Res. 190, 409–416 (1978). https://doi.org/10.1007/BF00219555
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DOI: https://doi.org/10.1007/BF00219555