Abstract
The effects of endotoxin or testosterone on the amount of transferrin 59Fe (or like protein) in the murine macrophages was investigated. The mouse peritoneal macrophages were laden with 59Fe tagged red cells following the injection of mice with either agent. After harvesting the cells, they were lysed and the transferrin iron was released with 40% trichloroacetic acid. The supernatant (extract transferrin iron) and the pellet (other iron proteins iron) were separated by centrifugation and their radioactivities counted. The results were expressed in percentage. The endotoxin group had a geometric mean of transferrin 59Fe of 0.14% compared to 0.28% for the control, p < 0.001. The geometric mean for transferrin 59Fe of the testosterone treated group was 0.51% compared to 0.35% for the control, p < 0.05. Therefore, the endotoxin seems to contract the transferrin pool whereas testosterone seems to expand it.
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References
Haurani FI, O'Brien R: A model system for the release of iron from the reticuloendothelial system. J Reticuloendothel Soc 12: 29–39, 1972
Custer G, Balcerzak S, Rinehart J: Human macrophage hemoglobin-iron metabolism in vitro. Am J Hematol 13: 23–36,1982
Haurani FI, Meyer A, O'Brien R: Production of transferrin by the macrophage. J Reticuloendothel Soc 14: 309–316, 1973
Reeves WB, Fairman RM, Haurani FI: Influence of hormones on the release of iron by macrophages. J Reticuloendothel Soc 29: 113–119, 1981
Pekarek RS, Wannemacher RW Jr, Beisel WR: The effect of leukocytic endogenous mediator (LEM) on the tissue distribution of zinc and iron. Proc Soc Exp Biol Med 140: 685–688,1972
Haurani FI, Quinn TJ: Modulators of macrophage transferrin or transferrin-like protein. XXI Congress of The International Society of Hematology, Sydney, Australia, 468: (abstract), 1986
Cohn ZA, Benzon B: The differentiation of mononuclear phagocytes. Morphology, cytochemistry and biochemistry. J Exp Med 121: 153–169, 1965
Winer BJ: Statistical Principles In Experimental Design. New York: McGraw-Hill Book Company, p. 111, 1962
Cruz WO, Hahn PF, Bale WF: Hemoglobin radioactive iron liberated by erythrocyte destruction (acetylphenylhydrazine) promptly reutilized to form new hemoglobins. Am J Physiol 135: 595–599, 1942
Lipschitz DA, Simon MO, Lynch SR, Dugard J, Bothwell TH, Charlton RW: Some factors affecting the release of iron from reticuloendothelial cells. Brit J Haemat 21: 289–303,1971
Haurani FI, Burke W, Martinez EJ: Defective reutilization of iron in the anemia of inflammation. J Lab Clin Med 65: 560–570,1965
Haurani FI, Green D: Primary defective iron reutilization: Response to testosterone therapy. Am J Med 42: 151–158, 1967
Haurani FI, Young K, Tocantins LM: Reutilization of iron in anemia complicating malignant neoplasms. Blood 22: 73–81,1963
Chrichton RR, Roman F, Roland F: Iron mobilization from ferritin by chelating agents. J Inorgan Biochem 13: 305–316, 1980
Treffry A, Harrison PM: Iron release from ferritin. Proc VII International Conference on Proteins of Iron Metabolism, A29: (Abstract), 1985
O'Shea MJ, Kershenobich D, Tavill AS: Effects of inflammation on iron and transferrin. metabolism: Brit J Haem 25: 707–714, 1973
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Haurani, F.I., Quinn, T.J. Modulators of macrophage transferrin or transferrin-like protein. Mol Cell Biochem 95, 49–53 (1990). https://doi.org/10.1007/BF00219529
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DOI: https://doi.org/10.1007/BF00219529