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Effects of hemin on a lymphoblastoid cell line that expresses the human ɛ- and γ-globin genes

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Summary

Northern blot analysis using probes specific for each of the human embryonic (ɛ), fetal (γ), and adult (β) globin genes indicates that the human lymphoblastoid F-265 cells express the embryonic and fetal globin genes. Unlike: the erythroid cell line K562, in which globin RNA levels increase during treatment with hemin in culture, globin RNA levels decrease in F-265 cells in the presence of hemin. This effect is reversible after passage of F-265 cells in fresh medium without hemin. Both the rates of globin RNA synthesis and the presence of DNase I-hypersensitive sites in hemin treated and untreated F-265 cells were investigated to identify the levels at which globin gene expression is controlled.

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Author notes

  1. Gary Brewer

    Present address: McArdle Laboratory for Cancer Research, University of Wisconsin, 450 North Randall, Ave., 53706, Madison, WI, USA

Authors and Affiliations

  1. Departments of Biochemistry and Pathology, the A. B. Hancock, Jr. Memorial Laboratory of the Vanderbilt University Cancer Center, and the Center in Molecular Toxicology, Vanderbilt University School of Medicine, 37232, Nashville, Tennessee, USA

    Gary Brewer, Lubomir S. Hnilica & Robert C. Briggs

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  1. Gary Brewer
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  2. Lubomir S. Hnilica
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  3. Robert C. Briggs
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Brewer, G., Hnilica, L.S. & Briggs, R.C. Effects of hemin on a lymphoblastoid cell line that expresses the human ɛ- and γ-globin genes. Mol Cell Biochem 68, 11–22 (1985). https://doi.org/10.1007/BF00219384

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  • DOI: https://doi.org/10.1007/BF00219384

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