Summary
The effects of the microtubular inhibitor, podophyllotoxin, on mitochondrial respiration were determined using isolated, digitonin-permeabilized hepatocytes and isolated mitochondria. In hepatocytes, podophyllotoxin (1.5 mM) inhibited coupled and uncoupled respiration of both FAD and NAD-linked substrates. In mitochondria, podophyllotoxin inhibited State III respiration, prevented the return to State IV respiration, and inhibited uncoupled respiration. There was no inhibition of ascorbate/TMPD oxidation in either the hepatocytes or the mitochondria. Podophyllotoxin had no effect upon oligomycin inhibition of coupled respiration. Oligomycin had no effect on the podophyllotoxin-inhibition of uncoupled respiration in either hepatocytes or mitochondria. The results indicate that podophyllotoxin alters electron flow at a site early in the electron transport chain.
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Horrum, M.A., Jennett, R.B., Ecklund, R.E. et al. Inhibition of respiration in mitochondria and in digitonin-treated rat hepatocytes by podophyllotoxin. Mol Cell Biochem 71, 79–85 (1986). https://doi.org/10.1007/BF00219331
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DOI: https://doi.org/10.1007/BF00219331