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Are opioid peptides co-localized with vasopressin or oxytocin in the neural lobe of the rat?

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Summary

The content of vasopressin, oxytocin, neurophysin, leucine-enkephalin, methionine-enkephalin, dynorphin-(1–13), and α-neoendorphin in the rat neurohypophysis was measured after different periods of dehydration and after depolarisation of isolated neural lobes and of neurosecretory nerve endings. The rates at which the amount of neurohypophysial hormone and opioid peptides decreased, and the changes in the ratios between the amount of vasopressin or oxytocin and opioid peptide in the neurohypophysis after dehydration and in the incubation medium after depolarization in vitro cast some doubt on, and can be explained by mechanisms other than co-localisation of the different peptides.

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References

  • Cazalis M, Dayanithi G, Nordmann JJ (1985) The role of patterned burst and interburst interval on the excitation-coupling mechanisms in the isolated rat neural lobe. J Physiol (Lond) 369:45–60

    Google Scholar 

  • Friesen HG, Astwood EB (1967) Changes in neurohypophysial proteins induced by dehydration and ingestion of saline. Endocrinology 80:278–287

    Google Scholar 

  • Giraud P, Castanas E, Patey G, Oliver C, Rossier J (1983) Regional distribution of methionine-enkephalin-Arg6-Phe7 in the rat brain: comparative study with the distribution of other opioid peptides. J Neurochem 41:154–160

    Google Scholar 

  • Goldstein A, Ghazarossian VE (1980) Immunoreactive dynorphin in pituitary and brain. Proc Natl Acad Sci USA 78:6207–6210

    Google Scholar 

  • Goldstein A, Fischli W, Lowney LJ, Hunkapiller M, Hood L (1981) Porcine pituitary dynorphin: complete amino acid sequence of the biologically active heptadecapeptide. Proc Natl Acad Sci USA 78:7219–7223

    Google Scholar 

  • Höllt V, Haarmann I, Bovermann K, Jerlicz M, Herz A (1980) Dynorphin-related immunoreactive peptides in rat brain and pituitary. Neurosci Lett 18:149–153

    Google Scholar 

  • Ito S, Iwanaga T, Yui R, Yamaguchi K, Hama H, Kamoi K, Shibata A (1981) Presence of α-neoendorphin-like immunoreactivity in the posterior lobe of the pituitary gland. Life Sci 29:1457–1461

    Google Scholar 

  • Kumar MSA, Chen CL, Muther TF (1979) Changes in the pituitary and hypothalamic content of methionine-enkephalin during the oestrus cycle of rats. Life Sci 25:1687–1696

    Google Scholar 

  • Land H, Schutz G, Schmale H, Richter D (1982) Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin-neurophysin II precursor. Nature 295:299–303

    Google Scholar 

  • Land H, Grez M, Ruppert S, Schmale H, Rehbein M, Richter D, Schutz G (1983) Deduced amine-acid sequence from the bovine oxytocin-neurophysin I precursor cDNA. Nature 302:342–344

    Google Scholar 

  • Legros JJ (1976) Les neurophysines. Masson, Paris

    Google Scholar 

  • Martin R, Geis R, Holl R, Schafer M, Voigt KH (1983) Co-existence of unrelated peptides in oxytocin and vasopressin terminals of rat neurohypophyses: immunoreactive methionine5-enkephalin-, leucine5-enkephalin- and cholecystokinin-like substances. Neuroscience 8:213–227

    Google Scholar 

  • Millan MJ, Millan MH, Herz A (1983) Contribution of the supraoptic nucleus to brain and pituitary pools of immunoreactive vasopressin and particular opioid peptides, and the interrelationships between these, in the rat. Neuroendocrinology 36:310–319

    Google Scholar 

  • Nakao K, Yoshimasa T, Oki S, Tanaka I, Nakai Y, Wakimasu M, Fujino M, Imura H (1981) Presence of dynorphin-like immunoreactivity in the rat pituitary gland and hypothalamus. Regul Pept 2:201–208

    Google Scholar 

  • Nordmann JJ, Morris JF (1984) Method for quantitating the molecular content of a subcellular organelle: Hormone and neurophysin content of newly formed and aged neurosecretory granules. Proc Natl Acad Sci USA 81:180–184

    Google Scholar 

  • Nordmann JJ, Dreifuss JJ, Legros JJ (1971) A correlation of release of polypeptide hormones and of immunoreactive neurophysin from isolated rat neurohypophyses. Experientia 27:1344–1345

    Google Scholar 

  • Nordmann JJ, Dayanithi G, Cazalis M (1986) Do opioid peptides modulate, at the level of the nerve endings, the release of neurohypophysial hormones? Exp Brain Res 61:560–566

    Google Scholar 

  • Rossier J, Vargo TM, Minick S, Ling N, Bloom FE, Guillemin R (1977) Regional dissociation of α-endorphin and enkephalin contents in rat brain and pituitary. Proc Natl Acad Sci USA 74:5162–5165

    Google Scholar 

  • Seitzinger BR, Maysinger D, Höllt V, Grimm C, Herz A (1982) Concomitant neonatal development and in vitro release of dynorphin and α-neoendorphin. Life Sci 31:1757–1760

    Google Scholar 

  • Usategui R, Oliver C, Vaudry H, Lombardi G, Rozenberg I, Mourre AM (1979) Immunoreactive α-MSH and ACTH levels in rat plasma and pituitary. Endocrinology 98:189–196

    Google Scholar 

  • Uttenthal LO, Livett BG, Hope DB (1971) Release of neurophysin together with vasopressin by a Ca++ dependent mechanism. Philos Trans R Soc Lond B 261:379–380

    Google Scholar 

  • Van Leeuwen FW, Pool C, Sluiter A (1983) Enkephalin immunoreactivity in synaptoid elements on glial cells in the rat neural lobe. Neuroscience 8:229–241

    Google Scholar 

  • Watson SJ, Akil H, Fischli W, Goldstein A, Zimmerman E, Nilaver G, Van Wimersma, Greidanus TB (1982) Dynorphin and vasopressin: common localization in magnocellular neurones. Science NY 216:85–87

    Google Scholar 

  • Weber E, Evans CJ, Barchas JD (1982) Predominance of the amino-terminal octapeptide fragment of dynorphin in rat brain regions. Nature 299:77–79

    Google Scholar 

  • Whitnall MH, Gainer H, Cox BM, Molineaux CJ (1983) Dynorphin-A-(1–8) is contained within vasopressin neurosecretory vesicles in rat pituitary. Science NY 222:1137–1139

    Google Scholar 

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Nordmann, J.J., Cazalis, M., Dayanithi, G. et al. Are opioid peptides co-localized with vasopressin or oxytocin in the neural lobe of the rat?. Cell Tissue Res. 246, 177–182 (1986). https://doi.org/10.1007/BF00219015

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