Summary
The formaldehyde-induced fluorescence method was used for light microscopy and fixation in glutaraldehyde and osmium tetroxide for electron microscopy to study the effect of 75 mg/kg 6-hydroxydopamine on the paracervical ganglion of the rat uterus. No changes were observed in the fluorescence intensity of the ganglion. Ultrastructurally the ganglion cells and the synapses remained unchanged. The small, intensely fluorescent (SIF) cells were severely damaged, displaying large vacuoles with an irregular contour as well as smaller, more regular vacuoles studded with ribosomes. The granular vesicles showed a lower density of their central parts suggesting that their catecholamine content had diminished.
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Angeletti, P. U., Levi-Montalcini, R.: Sympathetic nerve cell destruction in newborn mammals by 6-hydroxydopamine. Proc. nat. Acad. Sci. (Wash.) 65, 114–121 (1970)
Cheah, T. B., Geffen, L. B., Jarrott, B., Ostberg, A.: Action of 6-hydroxydopamine on lamb sympathetic ganglia, vas deferens and adrenal medulla: a combined histochemical, ultrastructural and biochemical comparison with the effects of reserpine Brit. J. Pharmacol. 42, 543–557 (1971)
Cobb, J.L.S., Bennett, T.: An electron microscopic examination of the short term effects of 6-hydroxydopamine on the peripheral adrenergic nervous system. In: 6-hydroxydopamine and catecholamine neurons, T. Malmfors and H. Thoenen, eds., p. 33–45. Amsterdam: North-Holland Pub., Co. 1971
Eränkö, L., Eränkö, O.: Effect of 6-hydroxydopamine on the ganglion cells and the small intensely fluorescent cells in the superior cervical ganglion of the rat. Acta physiol. scand. 84, 115–174 (1972)
Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehydeinduced fluorescence. J. roy. micr. Soc. 87, 259–276 (1967)
Eränkö, O.: Light and electron microscopic histochemical evidence of granular and nongranular storage of catecholamines in the sympathetic ganglion of the rat. Histochem. J. 4, 213–224 (1972)
Eränkö, O., Härkönen, M.: Histochemical demonstration of fluorogenic amines in the cytotoplasm of sympathetic ganglion cells of the rat. Acta physiol. scand. 58, 285–286 (1963)
Eränkö, O., Härkönen, M.: Monoamine containing small cells in the superior cervical ganglion of the rat and an organ composed of them. Acta physiol. scand. 65 511–512 (1965)
Hervonen, A., Kanerva, L.: Effect of 6-OH-dopamine on the autonomic nerves of the rabbit myometrium. Acta physiol. scand. 90, 107–112 (1974)
Hervonen, A., Kanerva, L., Teräväinen, H.: The fine structure of the paracervical ganglion of the rat after permanganate fixation. Acta physiol. scand. 85, 506–510 (1972)
Hökfelt, T.: Distribution of noradrenaline storing particles in peripheral adrenergic neurons as revealed by electron microscopy. Acta physiol. scand. 76, 427–440 (1969)
Hökfelt, T., Johnsson, G., Sachs, Ch.: Fine structure and fluorescence morphology of adrenergic nerves after 6-hydroxydopamine in vivo and in vitro. Z. Zellforsch. 131, 529–543 (1972)
Jonsson, G., Sachs, Ch.: Effects of 6-hydroxydopamine on the uptake and storage of noradrenalin in sympathetic adrenergic neurons. Europ. J. Pharmacol. 9, 141–155 (1970)
Kanerva, L.: The postnatal development of monoamines and cholinesterases in the paracervical ganglion of the rat uterus. Progr. Brain Res. 34, 433–444 (1971)
Kanerva, L.: Ultrastructure of sympathetic ganglion cells and granule-containing cells in the paracervical (Frankenhäuser) ganglion of the newborn rat. Z. Zellforsch. 126, 25–40 (1972a)
Kanerva, L.: Light and electron microscopical observations on the postnatal development of the paracervical (Frankenhäuser) ganglion of the rat. Z. Anat. Entwickl.-Gesch. 136, 33–50 (1972b)
Kanerva, L.: Development, histochemistry and connections of the paracervical (Frankenhäuser) ganglion of the rat uterus. A light and electron microscopical study. Acta inst. Anat. (Helsinki), Suppl. 2, 1–31 (1972c)
Kanerva, L., Hervonen, A.: New types of endoplasmic reticulum in neurons of the paracervical ganglion of the rat. Z. Zellforsch. 143, 505–515 (1973)
Kanerva, L., Lietzén, B., Teräväinen, H.: Catecholamines and cholinesterases in the paracervical (Frankenhäuser) ganglion of normal and pregnant rats. Acta physiol. scand. 86, 271–277 (1972)
Kanerva, L., Teräväinen, H.: Electron microscopy of the paracervical (Frankenhäuser) ganglion of the adult rat. Z. Zellforsch. 129, 161–177 (1972)
Laverty, R., Sharman, D. F., Vogt, M.: Action of 2, 4, 5-trihydroxyphenyl ethylamine on the storage and release of noradrenaline. Brit. J. Pharmacol. 24, 549–560 (1965)
Malmfors, T.: The effects of 6-hydroxydopamine on the adrenergic nerves as revealed by the fluorescence histochemical method. In: 6-hydroxydopamine and catecholamine neurons, T. Malmfors and H. Thoenen, eds. p. 47–58. Amsterdam: North Holland Pub. Co., 1971
Malmfors, T., Sachs, Ch.: Degeneration of adrenergic nerves produced by 6-hydroxydopamine. Europ. J. Pharmacol. 3, 89–92 (1968)
Matthews, M., Raisman, G.: The ultrastructure and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion. J. Anat. (Lond.) 105, 255–282 (1969)
Papka, R. E.: The ultrastructure of adrenergic neurons in sympathetic ganglia of the newborn rabbit after treatment with 6-hydroxydopamine. Amer. J. Anat. 137, 447–466 (1973)
Ploem, J. S.: The microscopic differentiation of the colour of formaldehyde induced fluorescence. Progr. Brain Res. 34, 27–38 (1971)
Porter, C. C., Totaro, J. A., Stone, C. A.: Effects of 6-hydroxydopamine and some other compounds on the concentration of norepinephrine in the hearts of mice. J. Pharmacol. exp. Ther. 140, 308–316 (1963)
Thoenen, H.: Biochemical alterations induced by 6-hydroxydopamine in peripheral adrenergic neurons. In: 6-hydroxydopamine and catecholamine neurons, T. Malmfors and H. Thoenen, eds, p. 75–85, Amsterdam: North-Holland Pub. Co. 1971
Thoenen, H., Tranzer, J. P. Chemical sympathectomy by selective destruction of adrenergic nerve endings with 6-hydroxydopamine. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 261, 172–288 (1968)
Tranzer, J. P., Richards, J. G.: Fine structural aspects of the effect of 6-hydroxydopamine on peripheral adrenergic neurons. In: 6-hydroxydopamine and catecholamine neurons, T. Malmfors and H. Thoenen, eds., p. 15–31. Amsterdam: North-Holland Pub. Co. 1971
Tranzer, J. P., Thoenen, H.: Ultramorphologische Veränderungen der sympathetischen Nervenendigungen der Katze nach Vorbehandlung mit 5-und 6-Hydroxy-Dopamine. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 257, 343–344 (1967)
Williams, T. H., Palay, S. L.: Ultrastructure of the small neurons in the superior cervical ganglion. Brain Res. 15, 17–34 (1969)
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Kanerva, L., Hervonen, A., Eränkö, O. et al. Fine structural changes caused by 6-OH-dopamine in the small intensely fluorescent cells of the paracervical ganglion of the rat. Cell Tissue Res. 152, 437–447 (1974). https://doi.org/10.1007/BF00218930
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DOI: https://doi.org/10.1007/BF00218930