Summary
Gastrin-and cholecystokinin (C.C.K.)-containing cells were detected by using anti-gastrin and anti-C.C.K. sera in the gastrointestinal tract of human fetuses and premature infants and in the stomach and duodenum of adult man obtained by biopsy from eight patients with normal gastro-duodenal endoscopy. The specificity of immunocytological reactions was ascertained by studying the inhibition of the reaction by gastrin, C.C.K., secretin, somatostatin, glucagon, insulin, serotonin, histamin, caerulein and octapeptide of C.C.K. In adult man, the gastrin cells are located only in the antrum and juxtapyloric region; C.C.K. was detected in the duodenum. In the human fetus, the first gastrin cells are seen in the antrum at 14 weeks of age and in the duodenum as early as 10 weeks; the C.C.K. cells are seen in the small intestine at 10 weeks of age.
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Bloom, S.R., Mortimer, C.H., Thorner, M.O., Besser, G.M., Hall, R., Gomez-Pan, A., Roy, V.M., Russell, R.C.G., Coy, D.H., Kastin, A.J., Schally, A.V.: Inhibition of gastrin and gastric-acid secretion by growth-hormone release-inhibiting hormone. Lancet 1974 II, 1106–1109
Dubois, P.M., Paulin, C., Assan, R., Dubois, M.P.: Human fetal pancreas: evidence for immunoreactive somatostatin in the endocrine cells. Nature (Lond.) 256, 731–732 (1975)
Dubois, P.M., Paulin, C., Dubois, M.P.: Gastrointestinal somatostatin cells in the human fetus. Cell Tissue Res. 166, 179–184 (1976)
Edkins, J.S.: On the chemical mechanism of gastric secretion. Proc. roy. Soc. Belg. 76, 376 (1905)
Goodfriend, T.L., Levine, L., Fasman, G.D.: Antibodies to bradykinin and angiotensin: a use of carbodiimides in immunology. Science 144, 1344–1346 (1964)
Harper, A.A., Raper, M.S.: Pancreozymin, a stimulant of the secretion of pancreatic enzymes in extracts of the small intestine. J. Physiol. (Lond.) 102, 115–125 (1943)
Hayes, J.R., Johnson, D.G., Koerker, D., Williams, R.H.: Inhibition of gastrin release by somatostatin in vitro. Endocrinology 96, 1374–1376 (1975)
Ivy, A.C., Oldberg, E.: A hormone mechanism for gallblader contraction and evacuation. Amer. J. Physiol. 86, 599–613 (1928)
Jorpes, E., Mutt, V., Toczko, K.: Further purification of cholecystokinin and pancreozymin. Acta chem. Scand. 18, 2408–2410 (1964)
Larsson, L.I., Håkanson, R., Sjöberg, N.O., Sundler, F.: Fluorescence histochemistry of the gastrin cell in fetal and adult man. Gastroenterology 68, 1152–1159 (1975)
McGuigan, J.E.: Gastric mucosal intracellular localization of gastrin by fluorescence. Gastroenterology 55, 315–327 (1968)
McGuigan, J.E., Greider, M.H.: Correlative immunochemical and light microscopic studies of the gastrin cell of the antral mucosa. Gastroenterology 60, 223–236 (1971)
Mutt, V., Jorpes, J.E.: Structure of porcine cholecystokinin-pancreozymin. 1. Cleavage with thrombin and with trypsin. Europ. J. Biochem. 6, 156–162 (1968)
Ondetti, M.A., Pluscec, J., Sabo, E.S., Sheehan, J.F., Williams, N.: Synthesis of cholecystokininpancreozymin. I. The C. terminal dodecapeptide. J. Amer. chem. Soc. 92, 195–199 (1970)
Pearse, A.G.E., Bussolati, G.: Immunofluorescence studies of the distribution of gastrin cells in different clinical stages. Gut 11, 646–648 (1970)
Polak, J.M., Pearse, A.G.E., Bloom, S.R., Buchan, A.M.J., Rayford, P.L., Thompson, J.C.: Identification of cholecystokinin-secreting cells. Lancet 1975 II, 1016
Sternberger, L.A., Hardy, P.H. Jr., Cucullis, J.J., Meyer, H.G.: The unlabeled antibody-enzyme method of immunochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)
Willems, G., Vansteenkiste, Limbosch, J.M.: Stimulating effect of gastrin on cell proliferation kinetics in canine fundic mucosa. Gastroenterology 62, 583–589 (1972)
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Acknowledgements. The authors should like to thank Professors Magnin and Liaras, Hôpital Edouard Herriot, M. Dumont, Hôpital de la Croix-Rousse, Notter et Garmier, Hôtel-Dieu, Bethenod, Hôpital Debrousse, Lyon, for their cooperation. We also thank Professor R. Guillemin, the Salk Institute, La Jolla, California, Doctor M.P. Dubois, I.N.R.A., Station de Physiologie de la Reproduction, Nouzilly, Mme Vagne, U. 45, I.N.S.E.R.M. and Professor Y. Minaire, Hôpital Edouard Herriot, Lyon, for their donations. This work was supported by a grant from the Institut National de la Santé et de la Recherche Médicale
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Dubois, P.M., Paulin, C. & Chayvialle, J.A. Identification of gastrin-secreting cells and cholecystokinin-secreting cells in the gastrointestinal tract of the human fetus and adult man. Cell Tissue Res. 175, 351–356 (1976). https://doi.org/10.1007/BF00218714
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DOI: https://doi.org/10.1007/BF00218714