Summary
Using the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the light microscopic level, it was demonstrated that, in the amphibian magnocellular hypothalamo-hypophysial neurosecretory system, vasotocin and mesotocin are synthesized in separate neurons. A tendency to preferential location of the two kinds of neuronal perikarya is described. The neurosecretory perikarya are the origin of separate vasotocinergic and mesotocinergic axons. In the neural lobe, the pattern of distribution of the two types of axons is different. The coarse ventricular “dendrites” of both kinds of neurons are hormone-containing processes. Staining with anti-bovine neurophysin I serum suggested that the vasotocinergic and the mesotocinergic neurons synthesize different neurophysins.
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Acher, R.: Chemistry of the neurohypophysial hormones: an example of molecular evolution. In: Handbook of physiology. Sect. 7: Endocrinology. Vol. IV. The pituitary gland and its neuroendocrine control, Part 1, pp. 119–130 (R.O. Greep and E.B. Astwood, eds.). Washington: American Physiological Society 1974
Aspeslagh, M.-R., Vandesande, F., Dierickx, K.: Electron microscopic immunocytochemical demonstration of separate neurophysin vasopressinergic and neurophysin-oxytocinergic nerve fibres in the neural lobe of the rat hypophysis. Cell Tiss. Res. 171, 31–37 (1976)
Dierickx, K.: The dendrites of the preoptic neurosecretory nucleus of Rana temporaria and the osmoreceptors. Arch. int. Pharmacodyn. 140, 708–725 (1962)
Dierickx, K., Vandesande, F.: Immuno-enzyme cytochemical demonstration of mesotocinergic nerve fibres in the pars intermedia of the amphibian hypophysis. Cell Tiss. Res. 1976 (in press)
Dierickx, K., Vandesande, F., Mey, J. De: Identification, in the external region of the rat median eminence of separate neurophysin-vasopressin and neurophysin-oxytocin containing nerve fibres. Cell Tiss. Res. 168, 141–152 (1976)
Mey, J. De, Dierickx, K., Vandesande, F.: Identification of neurophysin producing cells. III. Immunohistochemical demonstration of neurophysin I-producing neurons in the bovine infundibular nucleus. Cell Tiss. Res. 161, 219–224 (1975)
Mey, J. De, Vandesande, F., Dierickx, K.: Identification of neurophysin producing cells. II. Identification of the neurophysin I and the neurophysin II producing neurons in the bovine hypothalamus. Cell Tiss. Res. 153, 531–543 (1974)
Moens, L.: Isolation of neurohypophysial hormones of Rana temporaria. Nature (Lond.) 237, 268–269 (1972)
Moens, L.: Isolation of neurointermediate pituitary proteins of the frog (Rana temporaria) and their tentative identification as neurophysins. Gen. comp. Endocr. 22, 70–76 (1974)
Moriarty, G.C.: Adenohypophysis: Ultrastructural cytochemistry. A Review. J. Histochem. Cytochem. 21, 855–894 (1973)
Moriarty, G.C., Moriarty, C.M., Sternberger, L.A.: Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem. 21, 825–833 (1973)
Rodriguez, E.M.: Morphological and functional relationship between the hypothalamo-neurohypophysial system and cerebrospinal fluid. In: Aspects of neuroendocrinology, pp. 352–365 (W. Bargmann and B. Scharrer, eds.). Berlin-Heidelberg-New York: Springer 1970
Sternberger, L.A., Hardy, P.H. Jr., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)
Vandesande, F., Dierickx, K.: Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system of the rat. Cell Tiss. Res. 164, 153–162 (1975)
Vandesande, F., Dierickx, K.: Immuno-cytochemical demonstration of the inability of the homozygous Brattleboro rat to synthesize vasopressin and vasopressin-associated neurophysin. Cell Tiss. Res. 165, 307–316 (1976)
Vandesande, F., Dierickx, K., Mey, J. De: Identification of the vasopressin-neurophysin II and the oxytocin-neurophysin I producing neurons in the bovine hypothalamus. Cell Tiss. Res. 156, 189–200 (1975)
Watkins, W.B.: Immunocytochemical identification of neurophysin-secreting neurons in the hypothalamo-neurohypophysial system of some non-mammalian vertebrates. Cell Tiss. Res. 162, 511–521 (1975)
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This investigation was supported by a grant from the Belgian Nationaal Fonds voor Geneeskundig Wetenschappelijk Onderzoek
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Vandesande, F., Dierickx, K. Immunocytochemical demonstration of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system. Cell Tissue Res. 175, 289–296 (1976). https://doi.org/10.1007/BF00218707
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DOI: https://doi.org/10.1007/BF00218707