Skip to main content

Is mucus involved in biocrystallization?

Study of the intestinal mucus of the sea-water eel Anguilla anguilla L.

Summary

Freeze-dried intestinal mucus of sea-water-adapted eels was analysed by scanning electron microscopy (SEM) and X-ray microanalysis. Calcite crystals were observed in the mucus fibres; their concentration increased along the hindgut. Random SEM observations made in situ indicated that mucus fibres were involved in the genesis of these crystals. Calcium-rich mucus globules were found fused inside crystal matrices. Single typical rhombohedric crystals of various complexity appeared within the mucus framework. The steps of crystal biogenesis were reconstituted in in-vitro conditions.

This is a preview of subscription content, access via your institution.

References

  • Been JM, Bill PM, Lewis D (1979) Microstructure of gallstones. Gastroenterology 76:548–555

    Google Scholar 

  • Boquet E, Boronat A, Ramos-Cormenzana A (1973) Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon. Nature 246:527–528

    Google Scholar 

  • Crowther RS, Marriott C (1984) Counter-ion binding to mucus glycoproteins. J Pharm Pharmacol 36:21–26

    Google Scholar 

  • Fletcher CR (1978) Osmotic and ionic regulation in the cod (Gadus callarias L.) I Water balance. J Comp Physiol 124:149–155

    Google Scholar 

  • Jehl B, Bauer R, Dörge A, Rick R (1981) The use of propane/isopentane mixtures for rapid freezing of biological specimens. J Microsc 123:307–309

    Google Scholar 

  • Kirsch R, Humbert W, Simonneaux V (1985) The gut as an osmoregulatory organ: comparative aspects and special references to fishes. In: Gilles R, Gilles-Baillen M (eds) Transport processes, iono- and osmoregulation. Springer, Berlin Heidelberg New York Tokyo, pp 265–277

    Google Scholar 

  • Levy PE, Smith BF, La Mont JT (1984) Human gall-bladder mucin accelerates nucleation of cholesterol in artificial bile. Gastroenterology 87:270–275

    Google Scholar 

  • Lucas J, Prévôt L (1984) Synthèse de l'apatite par voie bactérienne à partir de matière organique phosphatée et de divers carbonates de calcium dans des eaux douce et marine naturelles. Chem Geol 42:101–118

    Google Scholar 

  • Noncollas GH (1979) The growth of crystals in solution. Adv Colloid Interface Sci 10:215–252

    Google Scholar 

  • Parmelee JT, Renfro JL (1983) Oesophageal deslination of sea water in flounder: role of active sodium transport. Am J Physiol 245:R 888-R 893

    Google Scholar 

  • Shedadeh ZH, Gordon MS (1969) The role of intestine salinity adaptation of the rainbow trout, Salmo gairdneri. Comp Biochem Physiol 30:397–418

    Google Scholar 

  • Shephard KL (1982) The influence of mucus on the diffusion of ions across the esophagus of fish. Physiol Zool 55(1): 23–24

    Google Scholar 

  • Simkiss K (1976) Intracellular and extracellular routes in biomineralization. In Calcium in biological systems, Cambridge University Press, London, pp 423–444

    Google Scholar 

  • Smith HW (1930) The absorption and excretion of water and salts by marine teleosts. Am J Physiol 93:480–505

    Google Scholar 

  • Williams RJP (1984) An introduction to biominerals and the role of organic molecules in their formation. Phil Trans R Soc Lond B 304:409–588

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Humbert, W., Kirsch, R. & Simonneaux, V. Is mucus involved in biocrystallization?. Cell Tissue Res. 245, 599–604 (1986). https://doi.org/10.1007/BF00218561

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00218561

Key words

  • Osmoregulation
  • Mucus
  • Intestine
  • Biocrystallization
  • Calcium
  • Anguilla anguilla