Summary
Freeze-dried intestinal mucus of sea-water-adapted eels was analysed by scanning electron microscopy (SEM) and X-ray microanalysis. Calcite crystals were observed in the mucus fibres; their concentration increased along the hindgut. Random SEM observations made in situ indicated that mucus fibres were involved in the genesis of these crystals. Calcium-rich mucus globules were found fused inside crystal matrices. Single typical rhombohedric crystals of various complexity appeared within the mucus framework. The steps of crystal biogenesis were reconstituted in in-vitro conditions.
Similar content being viewed by others
References
Been JM, Bill PM, Lewis D (1979) Microstructure of gallstones. Gastroenterology 76:548–555
Boquet E, Boronat A, Ramos-Cormenzana A (1973) Production of calcite (calcium carbonate) crystals by soil bacteria is a general phenomenon. Nature 246:527–528
Crowther RS, Marriott C (1984) Counter-ion binding to mucus glycoproteins. J Pharm Pharmacol 36:21–26
Fletcher CR (1978) Osmotic and ionic regulation in the cod (Gadus callarias L.) I Water balance. J Comp Physiol 124:149–155
Jehl B, Bauer R, Dörge A, Rick R (1981) The use of propane/isopentane mixtures for rapid freezing of biological specimens. J Microsc 123:307–309
Kirsch R, Humbert W, Simonneaux V (1985) The gut as an osmoregulatory organ: comparative aspects and special references to fishes. In: Gilles R, Gilles-Baillen M (eds) Transport processes, iono- and osmoregulation. Springer, Berlin Heidelberg New York Tokyo, pp 265–277
Levy PE, Smith BF, La Mont JT (1984) Human gall-bladder mucin accelerates nucleation of cholesterol in artificial bile. Gastroenterology 87:270–275
Lucas J, Prévôt L (1984) Synthèse de l'apatite par voie bactérienne à partir de matière organique phosphatée et de divers carbonates de calcium dans des eaux douce et marine naturelles. Chem Geol 42:101–118
Noncollas GH (1979) The growth of crystals in solution. Adv Colloid Interface Sci 10:215–252
Parmelee JT, Renfro JL (1983) Oesophageal deslination of sea water in flounder: role of active sodium transport. Am J Physiol 245:R 888-R 893
Shedadeh ZH, Gordon MS (1969) The role of intestine salinity adaptation of the rainbow trout, Salmo gairdneri. Comp Biochem Physiol 30:397–418
Shephard KL (1982) The influence of mucus on the diffusion of ions across the esophagus of fish. Physiol Zool 55(1): 23–24
Simkiss K (1976) Intracellular and extracellular routes in biomineralization. In Calcium in biological systems, Cambridge University Press, London, pp 423–444
Smith HW (1930) The absorption and excretion of water and salts by marine teleosts. Am J Physiol 93:480–505
Williams RJP (1984) An introduction to biominerals and the role of organic molecules in their formation. Phil Trans R Soc Lond B 304:409–588
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Humbert, W., Kirsch, R. & Simonneaux, V. Is mucus involved in biocrystallization?. Cell Tissue Res. 245, 599–604 (1986). https://doi.org/10.1007/BF00218561
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218561