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Alterations in the neurohypophysis of rats treated with chlorphentermine or tricyclic antidepressants

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Summary

Rats were treated with several amphiphilic, cationic compounds that are known to cause generalized lipidosis (chlorphentermine, iprindole, 1-chloro-amitriptyline, clomipramine). After prolonged drug treatment the neurohypophysis showed severe morphologic alterations particularly in Herring bodies (HB), perivascular cells, and pituicytes. HBs displayed the following abnormalities: (a) great accumulation of autophagic vacuoles that contained neurosecretory granules (NSG); (b) numerous coarse osmiophilic conglomerates; (c) masses of multilamellated material; (d) reduced numbers of intact NSGs. Perivascular cells accumulated large lamellated inclusion bodies. Pituicytes contained membrane-bound crystalloid inclusion bodies. The noxious effect of chlorphentermine and 1-chloro-amitriptyline was more pronounced than that of iprindole and clomipramine.

The alterations in perivascular cells and in pituicytes are typical of drug-induced lipidosis. The lesions in HBs are tentatively explained as follows: HBs were previously proposed to be the sites of normally occurring intraaxonal disposal of excess neurosecretory material. The present experimental conditions interfere with this catabolic process. Incomplete digestion of the axoplasmic constituents due for disposal might result in abnormal accumulation of NSG-containing autophagic vacuoles, osmiophilic conglomerates, and multilamellated material. This eventually leads to degeneration of HBs.

The functional implications of the neurohypophysial lesions remain to be elucidated by functional experiments.

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References

  • Bargmann, W.: Neurosecretion. Int. Rev. Cytol. 19, 183–201 (1966)

    Google Scholar 

  • Blümcke, S., Niedorf, H.R., Rode, J.: Axoplasmic alterations in the proximal and distal stumps of transected nerves. Acta neuropath. (Berl.) 7, 44–61 (1966)

    Google Scholar 

  • Dellmann, H.D.: Degeneration and regeneration of neurosecretory systems. Int. Rev. Cytol. 36, 215–315 (1973)

    Google Scholar 

  • Dellmann, H.D., Rodríguez, E.M.: Herring bodies; an electron microscopic study of local degeneration of neurosecretory axons. Z. Zellforsch. 111, 293–315 (1970)

    Google Scholar 

  • Dellmann, H.D., Stoeckel, M.E., Porte, A., Stutinsky, F., Chang, N., Adlinger, H.K.: Herring bodies reexamined: An ultrastructural experimental investigation of the rat neural lobe. Anat. Histol. Embryol. 3, 101–110 (1974)

    Google Scholar 

  • Douglas, W.W., Nagasawa, J., Schulz, R.: Electron microscopic studies on the mechanism of secretion of posterior pituitary hormones and significance of microvesicles (“synaptic vesicles”): Evidence of secretion by exocytosis and formation of microvesicles as a byproduct of this process. Mem. Soc. Endocr. 19, 353–379 (1971)

    Google Scholar 

  • Heap, P.F., Jones, C.W., Morris, J.F., Pickering, B.T.: Movement of neurosecretory product through the anatomical compartments of the neural lobe of the pituitary gland. Cell Tiss. Res. 156, 484–497 (1975)

    Google Scholar 

  • Holtzman, E., Novikoff, A.B.: Lysosomes in the rat sciatic nerve following crush. J. Cell Biol. 27, 651–669 (1965)

    Google Scholar 

  • Kalimo, H.: Ultrastructural studies on the hypothalamic neurosecretory neurons of the rat. III. Paraventricular and supraoptic neurons during lactation and dehydration. Cell Tiss. Res. 163, 151–168 (1975)

    Google Scholar 

  • Kodama, Y., Fujita, H.: Some findings on the fine structure of the neurohypophysis in dehydrated and pitressin-treated mice. Arch. histol. jap. 38, 121–131 (1975)

    Google Scholar 

  • Krisch, B.: Different populations of granules and their distribution in the hypothalamo-neurohypo-physial tract of the rat under various experimental conditions. Cell Tiss. Res. 151, 117–140 (1974)

    Google Scholar 

  • Lüllmann, H., Lüllmann-Rauch, R., Reil, G.H.: A comparative ultrastructural study of the effects of chlorphentermine and triparanol in rat lung and adrenal gland. Virchows Arch. Abt. B 12, 91–103 (1973a)

    Google Scholar 

  • Lüllmann, H., Lüllmann-Rauch, R., Wassermann, O.: Drug-induced phospholipidosis. Germ. Med. 3, 128–135 (1973b), translated from Dtsch. med. Wschr. 98, 1616–1625 (1973b)

    Google Scholar 

  • Lüllmann, H., Lüllmann-Rauch, R., Wassermann, O.: Drug-induced phospholipidoses. Critical Rev. Toxicol. 4, 185–218 (1975)

    Google Scholar 

  • Lüllmann-Rauch, R.: Lipidosis-like alterations in spinal cord and cerebellar cortex of rats treated with chlorphentermine or tricyclic antidepressants. Acta neuropath. (Berl.) 29, 237–249 (1974a)

    Google Scholar 

  • Lüllmann-Rauch, R.: Lipidosis-like alterations in hypothalamic neurosecretory cells of rats treated with chlorphentermine or iprindole. Cell Tiss. Res. 149, 587–590 (1974b)

    Google Scholar 

  • Lüllmann-Rauch, R.: Lipidosis-like renal changes in rats treated with chlorphentermine or with tricyclic antidepressants. Virchows Arch. Abt. B 18, 51–60 (1975)

    Google Scholar 

  • Olivieri-Sangiacomo, C.: On the fine structure of the perivascular cells in the neural lobe of rats. Z. Zellforsch. 132, 25–34 (1972)

    Google Scholar 

  • Olivieri-Sangiacomo, C.: Ultrastructural features of pituicytes in the neural lobe of adult rats. Experientia (Basel) 29, 1119–1120 (1973)

    Google Scholar 

  • Rufener, C.: Autophagy of secretory granules in the rat neurohypophysis. Neuroendocrinology 13, 314–320 (1974)

    Google Scholar 

  • Vilhardt, H.: Vasopressin content and neurosecretory material in the hypothalamo-neurohypophyseal system of rats under different states of water metabolism. Acta endocr. (Kbh.) 63, 585–594 (1970)

    Google Scholar 

  • Whitaker, S., LaBella, F.S.: Ultrastructural localization of acid phosphatase in the posterior pituitary of the dehydrated rat. Z. Zellforsch. 125, 1–15 (1972)

    Google Scholar 

  • Whitaker, S., LaBella, F.S., Sanwal, M.: Electron microscopic histochemistry of lysosomes in neurosecretory nerve endings and pituicytes of rat posterior pituitary. Z. Zellforsch. 111, (1970)

  • Zambrano, D., Robertis, De E.: The ultrastructural changes in the neurohypophysis after destruction of the paraventricular nuclei in normal and castrated rats. Z. Zellforsch. 88, 496–510 (1968)

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, University of Kiel, Kiel, Germany

    R. Lüllmann-Rauch

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  1. R. Lüllmann-Rauch
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This work was supported by the Deutsche Forschungsgemeinschaft (Lu 172/2) and the Stiftung Volkswagenwerk. The skilful technical assistance of Mrs. R. Worm is thankfully acknowledged

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Lüllmann-Rauch, R. Alterations in the neurohypophysis of rats treated with chlorphentermine or tricyclic antidepressants. Cell Tissue Res. 169, 501–514 (1976). https://doi.org/10.1007/BF00218149

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  • DOI: https://doi.org/10.1007/BF00218149

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