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Reconstitution of rat liver 60S ribosomal subunits following disassembly by dimethylmaleic anhydride

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Summary

Modification of 60S ribosomal subunits from rat liver with dimethylmaleic anhydride (60 μol/ml) is accompanied by release of 35% of the protein. The acidic ribosomal proteins, as well as 9 basic proteins, are selectively liberated from the ribosomal subunits. Reconstitution of the protein-deficient particles with the corresponding split proteins is accompanied by substantial recovery of the original polyphenylalanine synthetic activity. The described reconstitution procedure can be used to investigate the roles played by the released proteins and the functional similarities of proteins from different sources. Hybrid reconstitution of residual ribosomal particles from rat liver or yeast with the corresponding heterologous split proteins produces subunits which have incorporated heterologous proteins but are inactive in polyphenylalanine synthesis.

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Abbreviations

DMMA:

Dimethylmaleic Anhydride

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Authors and Affiliations

  1. Centro de Biología Molecular, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Canto Blanco, 28049, Madrid, Spain

    Sara Garrido, Pedro J. González, Enrique Palacián & Francisco Hernández

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  1. Sara Garrido
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  2. Pedro J. González
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  3. Enrique Palacián
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  4. Francisco Hernández
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Garrido, S., González, P.J., Palacián, E. et al. Reconstitution of rat liver 60S ribosomal subunits following disassembly by dimethylmaleic anhydride. Mol Cell Biochem 92, 159–167 (1990). https://doi.org/10.1007/BF00218133

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  • DOI: https://doi.org/10.1007/BF00218133

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