Skip to main content

Advertisement

SpringerLink
Log in

Effect of interferon alpha, interferon beta, and interferon gamma on the in vitro growth of human renal adenocarcinoma cells

  • Preclinical Studies
  • Published:
Investigational New Drugs Aims and scope Submit manuscript

Abstract

Interferon-α, interferon-β, and interferon-γ differ in their antiproliferative effects for several cell lines. Interferons were thus assessed for their activity in inhibiting proliferation of three renal cell carcinoma cell lines. The malignant epithelial phenotype of each of these cell lines was confirmed by electron microscopy, histology, karyotype and tumorigenicity. When compared on an anti-viral unit basis, naturally produced interferon-β was more effective than natural interferon-α for all cell lines and clones. Proliferation of each of the cell lines was inhibited by interferon-γ. In all cases, removal of interferons from culture media resulted in resumption of the rate of cell growth after a variable delay of 6–10 days. If the antiproliferative effects of interferons predominate in mediating tumor regression, clinical response may depend upon the type of interferon to which the tumor is exposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gutterman JU, Blumenschein GR, Alexanian R, Yap H-Y, Buzdar AU, Cabanillas F, Hortobagyi GN, Hersh EM, Rasmussen SL, Harmon M, Kramer M, Pestka S,: Leukocyte interferon-induced regressions in human metastatic breast cancer, multiple myeloma and malignant lymphoma. Ann Int Med 93:399–406, 1980

    Google Scholar 

  2. Louie AC, Gallagher JG, Sikora K, Levy R, Rowenberg SA, Merigan TC: Follow-up observations on the effect of human leukocyte interferon in non-Hodgkin's lymphoma. Blood 58:712–718, 1981

    Google Scholar 

  3. Osserman EF, Sherman WH, Alexanian R, Gutterman JV, Humphrey RI: Human leukocyte interferon in multiple myeloma. In: Demaeyer E, Galasso G, Schellekens H (eds): The Biology of the Interferon System. Elsevier/ North Holland Biomedical Press, New York, 1981, pp 409–413

    Google Scholar 

  4. Borden EC, Holland JF, Dao TL, Gutterman JU, Weiner L, Chang Y-C, Patel J: Leukocyte-derived interferon (alpha) in human breast carcinoma. Ann Int Med 97:1–6, 1982

    Google Scholar 

  5. De Kernion JB, Sarna G, Figlin R, Lindner A, Smith RB: The treatment of renal cell carcinoma with human leukocyte alpha-interferon. J Urol 130:1063–1066, 1983

    Google Scholar 

  6. Neidhart JA, Gagen MM, Young D, Tuttle R, Melink TJ, Ziccarrelli A, Kisner D: Interferon-α therapy of renal can cer. Cancer Res 44:4140–4143, 1984

    Google Scholar 

  7. Quesada JR, Swanson DA, Trindade A, Gutterman JU: Renal cell carcinoma: Antitumor effects of leukocyte interferon. Cancer Res 43:930–947, 1983

    Google Scholar 

  8. Trump D, Harris J, Tuttle R, Oken M, Bennett J, Magers O, Davis T: Phase II trial of high dose human lympho blastoid interferon (WellferonR) in advanced renal cell carcinoma (RCC) and ECOG pilot. Proc Am Soc Clin Onc (Abstr) 3:153, 1984

    Google Scholar 

  9. Borden EC, Hogan TF, Voelker JG: The comparative antiproliferative activity in vitro of natural interferons α and β for diploid and transformed human cells. Cancer Res 42:4948–4953, 1982

    Google Scholar 

  10. Kataoka T, Oh-Hashi F, Sakunai Y, Ida N: Characteristics of in vitro antiproliferation activity of human interferon-β. Cancer Chemother Pharmacol 9:75–80, 1982

    Google Scholar 

  11. Chang YC, Hogan TF, Borden EC: Preliminary characterization of ACHN, a human renal adenocarcinoma cell line growth inhibited by human interferons (IFN) α and β. (Abstr) Proc. Am Assoc Cancer Res 24:4, 1983

    Google Scholar 

  12. Seljelid R, Ericsson JLE: Electron microscopic observation on specializations of the cell surface in renal clear cell carci noma. Lab Invest 14:435–447, 1965

    Google Scholar 

  13. Rohatiner AZS, Balkwill FR, Griffin DB, Malpas JS, Lister TA: Aphase I study of human lymphoblastoid interferon administered by continuous intravenous infusion. Cancer Chemother Pharmacol 9:97–102, 1982

    Google Scholar 

  14. Sherwin SA, Knost JA, Fein S, Abrams PG, Foon KA, Ochs JJ, Schoenberger C, Maluish AE, Oldham RK: A multiple-dose phase I trial of recombinant leukocyte α-interferon in cancer patients. J Am Med Assoc 248:2461–2466, 1982

    Google Scholar 

  15. Salmon SE, Durie BGM, Young L, Liu RM, Trown PW, Stebbing N: Effects of cloned human leukocyte interferons in the human tumor stem cell assay. J Clin Oncol 1:217–225, 1983

    Google Scholar 

  16. Von Hoff DD, Gutterman J, Portnoy B, Coltman Jr CA: Activity of human leukocyte interferon in a human tumor cloning system. Cancer Chemother Pharmacol 8:99–103 1982

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Humart Oncology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    J. Philip Kuebler

  2. Department of Pathology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    Terry D. Oberley

  3. Department of Cytogenetics, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    Lorraine F. Meisner

  4. Department of Human Oncology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    Younan A. Sidky

  5. Department of Human Oncology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    Catherine A. Reznikoff

  6. Department of Human Oncology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    Ernest C. Borden

  7. Department of Urology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    Kenneth B. Cummings

  8. Department of Human Oncology, University of Wisconsin Center for Health Sciences, 53792, Madison, Wisconsin, USA

    George T. Bryan

Authors
  1. J. Philip Kuebler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Terry D. Oberley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lorraine F. Meisner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Younan A. Sidky
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Catherine A. Reznikoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ernest C. Borden
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kenneth B. Cummings
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. George T. Bryan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuebler, J.P., Oberley, T.D., Meisner, L.F. et al. Effect of interferon alpha, interferon beta, and interferon gamma on the in vitro growth of human renal adenocarcinoma cells. Invest New Drugs 5, 21–29 (1987). https://doi.org/10.1007/BF00217665

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00217665

Key words

Search

Navigation