Abstract
The interaction of avidin (from egg white) with phospholipid (monolayer and bilayer) model membranes containing biotin-conjugated phospholipids has been studied. In the first part, using surface sensitive techniques (ellipsometry and surface plasmon resonance) we demonstrated that the nonspecific adsorption of avidin to phospholipid lamella could be abolished by adding an amount of Ca2+, Mg2+, or Ba2+ that led to an electrostatic interaction. The specific binding of avidin to lipid mixtures containing biotin-conjugated phospholipids was obviously composition dependent. The ratio 1:12 of a B-DPPE/DPPE mixture was found to be the optimum molar ratio. When we compared the results from the surface sensitive techniques with those from the electron micrographs of a two dimensional crystal of avidin (obtained in our laboratory), the optimum ratio was found to be determined by the effect of lateral steric hindrance. In the second part, we observed the pattern of the layers of fluorescently labeled phospholipid and adsorbed proteins with a home-made micro fluorescence film balance. The fluorescence images showed that avidin was preferentially bound to the receptors that were in the fluid domains. Further, with a sensitive fluoresence assay method, the effect of the phase behavior of liposomes on the specific binding of avidin was measured. This showed that avidin interacted with biotin-lipid more weakly in the gel state liposome than in the liquid state liposome. The major conclusion was that the binding of avidin to a membrane bound model receptor was significantly restricted by two factors: one was the lateral steric hindrance and the other was the fluidity of the model membrane.
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Abbreviations
- B-DPPE:
-
Biotinyl dipalmitoylphosphatidyl ethanolamine
- B-DMPE:
-
Biotinyl dimyristoylphosphatidyl ethanolamine
- BNHS:
-
d-biotin-N-hydroxysuccinimide ester
- DMPA:
-
dimyristoylphosphatidyl acid
- DMPC:
-
dimyristoylphosphatidyl choline
- DMPS:
-
dimyristoylphosphatidyl serine
- DOPC:
-
dioleoylphosphatidyl choline
- DPPC:
-
dipalmitoylphosphatidyl choline
- DPPE:
-
dipalmitoylphosphatidyl ethanolamine
- FITC:
-
fluorescein isothiocyanate
- RDB-DOPE:
-
N(Lissamine rhodamine B sulfonyl) dioleoyl phosphatidylethanolamine
- SPR:
-
surface plasmon resonance
References
Ahlers M, Miller W, Reichert A, Ringdorf H, Venzmer J (1990) Specific interactions of proteins with functional lipid monolayers —Ways of simulating biomembrane processes. Angew Chem Int Ed Engl 29:1269–1285
Argarana CE, Kuntz ID, Birken S, Axel R, Cantor CR (1986) Molecular cloning and nuleotide sequence of the streptavidin gene. Nucl Acids Res 14:1871–1882
Azzam RMA, Bashara NM (1977) In: Ellipsometry and polarized light. North-Holland, Amsterdam
Bayer EA, Wilchek M (1990) The use of the avidin-biotin complex. Methods Biochem Anal 26:1–45
Bayer EA, Rivany B, Skutelsky E (1979) On the mode of liposome-cell interactions biotin-conjugated lipids as ultrastructural probes. Biochim Biophys Acta 550:464–473
Blankenburg R, Meller P, Ringsdorf H, Salesse C (1989) Interaction between biotin lipids and streptavidin in monolayers: formation of oriented two-dimensional protein domains induced by surface recognition. Biochemistry 28:8214–8221
Bruch RC, White III HB (1982) Compositional and structural heterogeneity of avidin glycopeptides. Biochemistry 21:5334–5341
Darst SA, Ahlers M, Meller PH, Kubalek EW, Blankenburg R, Ribi HO, Ringsdorf H, Kornberg RD (1991) Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules. Biophys J 59:387–396
Egger M, Heyn P, Gaub HE (1990) Two-dimensional recognition pattern of lipid-anchored Fab'-fragments. Biophysics J 57: 669–673
Fettiplace R, Andrews DM, Haydon DA (1971) The thickness, composition and structure of some lipid bilayers and natural membranes. J Membr Biol 5:277–296
Gabrielli G (1991) Monolayers and planar or curved bilayers. Adv Colloid Interface Sci 34:31–72
Gaub HE, May VT, McConnell HM (1986) Reversible formation of plastic two-dimensional lipid crystals. J Phys Chem 90: 1721–1725
Green NM (1963) Avidin: the nature of the biotin-binding site. Biochem J 89:599–609
Haydon DA, Hladky SB (1972) Ion transport across thin lipid membranes: A critical discussion of mechanisms in selected system. Quart Rev Biophys 5:187–282
Israelachvili JN (1985) In: Intermolecular and surface forces. Academic Press, London
Jönsson U, Ivarsson B, Lundström I, Berghem L (1982) Adsorption behavior of fibroection on well-characterized silica surfaces. J Colloid Interface Sci 90:148–163
Kornberg RD, Darst SA (1991) Two-dimensional crystals of proteins on lipid layers. Curr Opinion Struct Biol 1:642–646
Kuhn H (1989) Present status and future prospects of Langmuir-Blodgett film research. Thin Solid Film 178:1–16
Levine YK, Wilkins MHF (1971) Structure of oriented lipid layers. Nature (London). New Biol 230:69–72
Lin HJ, Kirsch JF (1977) A sensitive fluorometric assay for avidin and biotin. Anal Biochem 81:442–446
Loesche M, Sackmann E, Möhwuld H (1983) A fluorescence microscopic study concerning the phase diagram of phospholipids Ber Bunsen- Ges Phys Chem 87:848–852
McConnel HM, Watts TH, Weis RM, Brian AA (1986) Supported planar membranes in studies of cell-cell recognition in the immune system. Biochim Biophys Acta 864:95–106
Melanmed MD, Green NM (1963) Avidin: purification and composition. Biochem 189:591–598
Qin H, Xie WZ, Sui SF (1992) Study on interaction of avidin with biotin containing lipid monolayer. Chinese Sci Bull 37: 1037–1040
Reather H (1988) Surface plasma on smooth and rough surface and gratings. In: Springer tracts in morden physics 111. Springer, Berlin Heidelberg
Schmidt CF, Zimmermann RM, Gaub HE (1990) Multilayer adsorption of lysozyme on a hydrophobic substrate. Biophys J 57: 577–588
Shinitzky M (1987) In: The lipid regulation of receptor function. In: Membrane and receptor mechanisms. Bertoli E, Chapman D (eds) Fidia Research Series, Vol. 7. Liviana Press, Padova, pp 135–141
Silver BL (1985) In: The Physical Chemistry of Membrane Allen & Unwin and The Soloman Press, pp 1–24
Sui SF, Urumow T, Sackmann E (1988) Interaction of insulin receptors with lipid bilayers and specific and nonspecific binding of insulin to supported membrane. Biochemistry 27:7463–7469
Uzgiris EE, Kornberg RD (1983) Two-dimensional crystallization technique for imaging macromolecules with application to antigen-antibody-complement complexes. Nature (London) 301: 125–129
Zhao S, Reichert WM (1992) Influence of biotin lipid surface density and accessibility on avidin binding to the tip of an optical fiber sensor. Langmuir 8:2785–2791
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Correspondence to: S. F. Sui
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Liu, Z., Qin, H., Xiao, C. et al. Specific binding of avidin to biotin containing lipid lamella surfaces studied with monolayers and liposomes. Eur Biophys J 24, 31–38 (1995). https://doi.org/10.1007/BF00216828
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DOI: https://doi.org/10.1007/BF00216828