Summary
After injection of radiolabeled 1,25 (OH)2 vitamin D3, nuclear concentration of radioactivity is observed in parenchymal cells of the parathyroid gland in pregnant, adult male, and 10-day male neonatal rats. In competition studies with unlabeled 1,25 (OH)2 vitamin D3, but not with 25 (OH) vitamin D3, nuclear uptake is prevented. Experiments with 3H 25 (OH) vitamin D3, in contrast to 3H 1,25 (OH)2 vitamin D3, do not show nuclear concentration in cells of the parathyroid. The results of the autoradiographic studies suggest the presence of receptors for a direct effect of 1,25 (OH)2 vitamin D3 on the parathyroid gland for modulation of parathyroid hormone secretion.
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Altenähr E, Dietel M, Dorn G, Montz R (1977) The effect of 1,25-dihydroxycholecalciferol on the parathyroid hormone secretion of porcine parathyroid glands and human parathyroid adenomas in vitro. Acta Endocrinol (Kbh) 86:533–538
Au WY-W, Bukowski A (1976) Inhibition of PTH secretion by vitamin D metabolites in organ cultures of rat parathyroids. Fed Proc 35:530
Brumbaugh PF, Hughes MR, Haussler MR (1975) Cytoplasmic and nuclear binding components for 1α, 25-dihydroxyvitamin D3 in chick parathyroid glands. Proc Natl Acad Sci USA 72:4871–4875
Chertow BS, Baylink DJ, Wergedal JE, Su MHH, Norman AW (1975) Decrease in serum immunoractive parathyroid hormone in rats and in parathyroid secretion in vitro by 1,25-dihydroxycholecalciferol. J Clin Invest 56:668–678
DeLuca HF (1978) The hormonal nature of vitamin D function. In: Dumont J, Nunez J (eds) Hormones and Cell Regulation. Biomedical Press, Elsevier, Amsterdam, North Holland, pp 249–270
DeLuca HF, Tanaka Y, Castillo L, Kumar R (1977) 1,25-dihydroxyvitamin D3: its further metabolism and the regulation of its biogenesis. In: Norman AW, Schaefer JW, Coburn JW, DeLuca HF, Fraser D, Grigoleit HG, Herrath DV (eds) VitaminD. Biochemical and clinical aspects related to calcium metabolism. DeGruyter, Berlin, pp 113–122
Dietel M, Dorn G, Montz R, Altenähr E (1979) Influence of vitamin D3,1,25-dihydroxyvitamin D3, and 24,25-dihydroxyvitamin D3 on parathyroid hormone secretion, adenosine 3′, 5′-monophosphate release, and ultrastructure of parathyroid glands in organ culture. Endocrinology 105:237–245
Garabedian M, Holick MF, DeLuca HF, Boyle IT (1972) Control of 25-hydroxycholecalciferol metabolism by parathyroid glands. Proc Natl Acad Sci USA 69:1673–1676
Habener JF, Kemper BW, Rich A, Potts JT (1977) Biosynthesis of parathyroid hormone. Recent Prog Horm Res 33:249–299
Haussler MR, Hughes MR, McCain TA, Pike JW (1977) 1,25-dihydroxyvitamin D: molecular mechanism, physiological regulation and clinical implications. In: MacIntyre I, Szelke M (eds) Molecular Endocrinology. Biochemical Press, Elsevier, Amsterdam, North Holland, pp 101–116
Henry HL, Taylor AN, Norman AW (1977) Response of chick parathyroid glands to the vitamin D metabolites, 1,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol. J Nutr 107:1918–1926
Hughes MR, Haussler MR (1978) 1,25-dihydroxyvitamin D3 receptors in the parathyroid glands. J Biol Chem 253:1065–1073
Kodicek E (1973) Vitamin D and the skeleton. In: Hard tissue growth, repair and remineralization. Ciba Found 11:359–371
MacIntyre I, Colston KW, Szelke M, Spanos E (1978) A survey of hormonal factors that control calcium metabolism. Ann NY Acad Sci 307:345–355
Narbaitz R, Stumpf WE, Sar M (1981) The role of autoradiographic and immunocytochemical techniques in the clarification of sites of metabolism and action of vitamin D. J Histochem Cytochem 29:91–100
Sherwood LM, Lundberg WB, Targovnik JH, Rodman JS, Seyfer A (1971) Synthesis and secretion of parathyroid hormone in vitro. Am J Med 50:658–669
Spanos F (1978) New aspects on the regulation of vitamin D metabolism. In: Dumont J, Nunez J (eds) Hormones and cell regulation, Vol. 2, pp 269–281
Stumpf WE (1971) Probable sites for estrogen receptors in brain and pituitary. J Neuro-Visc Relat Suppl X:51–64
Stumpf WE, Sar M (1975) Autoradiographic techniques for localizing steroid hormones. In: O'Malley BW, Hardman JG (eds) Methods in enzymology, Vol. XXXVI. Hormone action Part A. Steroid hormones. Academic Press, New York, pp 135–156
Stumpf WE, Sar M (1976) Autoradiographic localization of estrogens, androgens, progesterone and glucocorticoids in “target tissues” and “non-target tissues”. In: Pasqualini WJ (ed) Receptors and mechanisms of action of steroid hormones, Part I. Marcel Dekker, New York, pp 41–84
Stumpf WE, Sar M, Reid FA, Tanaka Y, DeLuca HF (1979) Target cells for 1,25-dihydroxy vitamin D3 in intestinal tract, stomach, kidney, skin, pituitary and parathyroid. Science 206:1188–1190
Stumpf WE, Sar M, DeLuca HF (1981) Sites of action of 1,25 (OH)2 vitamin D3 identified by thawmount autoradiography. In: Cohn DW, Talmage RV, Matthews JL (eds) Hormonal control of calcium metabolism. Elsevier, Amsterdam, North Holland, pp 222–229
Suda T, DeLuca HF, Tanaka Y (1970) Biological activity of 25-hydroxycholecalciferol in rats. J Nutr 100:1049–1052
Wasserman RH, Corradino RA (1973) Vitamin D, calcium and protein synthesis. Vitam Horm 31:42–103
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Stumpf, W.E., Sar, M., Reid, F.A. et al. Autoradiographic studies with 3H 1,25 (OH)2 vitamin D3 and 3H 25 (OH) vitamin D3 in rat parathyroid glands. Cell Tissue Res. 221, 333–338 (1981). https://doi.org/10.1007/BF00216737
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DOI: https://doi.org/10.1007/BF00216737