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Precocious development of lectin (Ulex europaeus agglutinin I) receptors in dome epithelium of gut-associated lymphoid tissues

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Summary

Dome epithelium (DE), the tissue covering lymphoid domes of gut-associated lymphoid tissues, was examined in both adult and neonatal rabbit appendix or sacculus rotundus to determine if dome epithelial cells matured earlier than epithelial cells covering adjacent villi. The localization of well-differentiated epithelial cells in rabbit gut-associated lymphoid tissues (GALT) was accomplished histochemically by use of molecular probes: fluorescein isothiocyanate or horseradish peroxidase conjugates of Ulex europaeus agglutinin I (UEA), a lectin specific for terminal L-fucose molecules on certain glycoconjugates. The villus epithelial cells of newborn and 2-, 5-, or 10-day-old rabbits did not bind UEA, but between the twelfth and fifteenth days of postnatal life, UEA receptors were expressed by well-differentiated villus epithelial cells. In contrast to villus epithelium, DE in appendix and sacculus rotundus of neonatal rabbits expressed UEA receptors two days after birth, a feature that distinguished the DE of neonatal GALT for the next two weeks. In adult rabbits, UEA receptors were associated with dome epithelial cells extending from the mouths of glandular crypts to the upper domes; in contrast to the domes, UEA receptors were only present on well-differentiated epithelial cells at the villus tips. Results suggested that in neonatal rabbits most dome epithelial cells developed UEA receptors shortly after birth, reflecting precocious development of DE as compared to villus epithelium. In adult rabbit dome epithelium UEA receptors appeared on dome epithelial cells as they left the glandular crypts, representing accelerated epithelial maturation.

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Abbreviations

DE:

dome epithelium

DEL:

dome epithelial lymphocytes

FITC:

fluorescein isothiocyanate

HRP:

horseradish peroxidase

PBS:

phosphate-buffered saline

PBS-CaMg:

PBS containing calcium and magnesium ions

UEA:

Ulex europaeus agglutinin I

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Authors and Affiliations

  1. Department of Experimental Pathology, Division of Pathology, Walter Reed Army Institute of Research, Washington, District of Columbia, USA

    Michael J. Roy

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  1. Michael J. Roy
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The views of the authors expressed here do not purport to reflect the position of the Department of the Army or the Department of Defense

In conducting the research described in this report, the investigators adhered to standards set forth in the “Guide for the Care and Use of Laboratory Animals” (NIH Publication 85-23) as promulgated by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council, USA

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Roy, M.J. Precocious development of lectin (Ulex europaeus agglutinin I) receptors in dome epithelium of gut-associated lymphoid tissues. Cell Tissue Res. 248, 483–489 (1987). https://doi.org/10.1007/BF00216473

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