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Effects of administration of recombinant human interleukin-2 in dogs

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Abstract

The clinical and immunohaeamatological effects of recombinant humen interleukin-2 (rhIL-2) administration were evaluated in normal dogs. Three groups of three dogs per group were administered rhIL-2 subcutaneously at a dose of 6 × 104 IU, 6 × 105 IU, or 6 × 106 IU/kg once daily for five consecutive days. Toxic clinical signs were limited primarily to diarrhoea, the severity of which, was dose dependent, with resolution within 7 days of the last rhIL-2 injection. Marked circulating eosinophilia occurred in dogs of the two highest dose groups and transient rise in blood lymphocyte numbers occurred in dogs given the highest dose of rhIL-2. The most significant immunological effects were elevated in vitro conA and pokeweed mitogen-stimulated lymphocyte blastogenic responsiveness in the highest dose group and dose-dependent elevation of antigen-specific antibody (IgG and IgM) production. Peak relative antibody production was markedly elevated, as compared to controls, in dogs administered 6 × 105 IU, 6 × 106 IU rhIL-2/kg.

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Authors and Affiliations

  1. Laboratory for Energy Related Health Research, University of California, Davis

    G. R. Cain, T. Kawakami & N. Taylor

  2. Transplantation Biomedical Unit, University of California, Los Angeles, California, USA

    R. Champlin

Authors
  1. G. R. Cain
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  2. T. Kawakami
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  3. N. Taylor
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  4. R. Champlin
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Cain, G.R., Kawakami, T., Taylor, N. et al. Effects of administration of recombinant human interleukin-2 in dogs. Comparative Haematology International 2, 201–207 (1992). https://doi.org/10.1007/BF00216095

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  • DOI: https://doi.org/10.1007/BF00216095

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