Abstract
The pulmonary intravascular macrophages of goat contain a unique electron-dense coat consisting of globular units arranged in a linear fashion along the cell surface and closely conforming to the contours of the cell. The coat is sensitive to heparin treatment and to the digestive effect of lipolytic lipase, suggesting that the coat is predominantly composed of lipoproteins. During the present study, the globules of the coat showed a high affinity for cationised ferritin within 2–5 min of intravenous injection. The adherence of cationised ferritin molecules to the globular units of the surface-coat was accompanied by platelet aggregation followed by their rapid phagocytosis by the pulmonary intravascular macrophages. Furthermore, large vesicular-structures were also seen in the phagosomes of the pulmonary intravascular macrophages, sometimes lying side by side with the engulfed platelets. It is suggested that cationised ferritin as a multivalent substance acted as a cross-link between negatively charged membrane components of the platelets and cationised globular units of the surface coat of the PIMs. The electrostatic attraction thus created between the two cells led to adhesion and subsequent phagocytosis of platelets as well as of anionic plasma proteins by the pulmonary intravascular macrophages.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atwal OS, Minhas KJ (1992). In vivo interaction of cationized ferritin CF with the surface coat and endocytosis by pulmonary intravascular macrophages (PIMs): A tracer kinetic study. J Anat (In press)
Atwal OS, Minhas KJ, Ferenczy BG et al. (1989) Morphology of pulmonary intravascular macrophages (PIMs) ultrastructural and cytochemical behaviour of dense surface coat. Am J Anal 186:285–299
Atwal OS, Minhas KJ, Gill BS et al. (1992a) In vivo monastral blue-induced lamellar bodies in lysosomes of pulmonary intravascular macrophages (PIMs) of bovine lungs: implications of the surface coat. Anat Rec 234:223–239
Atwal OS, Singh B, Staempfli H et al. (1992b) Presence of pulmonary intravascular macrophages in the equine lung: Some Sructuro-functional properties. Anat Rec 234:530–540
Bertram TA, Elnig TE, Brody AR (1987) Stimulation of arachidonic acid metabolism in pulmonary intravascular macrophages: comparison of products induced by particulate and soluble agents. Am Rev Resp Dis 137:A88
Bertram TA, Overby LH, Damilowica et al. (1988) Pulmonary intravascular macrophages metabolize arachidonic acid in vitro: comparison to alveolar macrophages. Am Rev Resp Dis 138:936–944
Carvallo TU, deSouza W (1990) Internalization of surface anionic sites and phagosomes — lysosomes fusion during interaction of Taxoplasma gondii with macrophages. Eur J Cell Biol 51:211–219
Citra WM, Silva-Filho FC, deSouza W (1986) The surface charge of Toxoplasma gondii: a cytochemical and electrophoretic study. J Submicrosc Cytol 18:773–781
Donald K (1980) Ultrastructure of reticuloendothelial clearance. In: Carr I, Daems WT (eds) The reticuloendothelial system, vol 1. Plenum Press, New York, pp 525–554
Emeis JJ (1976) Morphologic and cytochemical heterogeneity of rat liver Kupffer cells. J Reticuloendothelial Soc 20:31–35
Emeis JJ, Barderoo P (1980) The cell coat of macrophages. In: Carr I, Daems WT (eds) The reticuloendothelial system, vol 1. Plenum Press, New York, pp 129–160
Farquhar MG (1978) Recovery of surface membrane in anterior pituitary cells. Variations in traffic detected with anionic and cationic ferritin. J Cell Biol 77:R35-R42
Fitzgerald LA, Phillips DR (1989) Structure and functions of platelet membrane glycoproteins. In: Kanicki TJ, George JN (eds) Platelet immunobiology. JB Lippincott, Philadelphia, pp 9–30
Jassal DS (1989) Ultrastructural and cytochemical studies on pulmonary intravascular macrophages in sheep. MSc Thesis,University of Guelph, Guelph, Canada
Kaplan JE (1981) The role of endothelial system in control of hemostatic and thrombotic mechanisms. In: Altura BM and Saba TM (eds) Pathophysiology of the reticuloendothelial system, Raven Press, New York, pp 111–127
Kreiger ML, Smith L, Anderson R et al. (1979) Reconstituted low density lipoprotein: a vehicle for the delivery of hydrophobic fluorescent probes in cells. J Supramol Struct 10:467–478
Longworth KE, Lei D, Westgate A et al. (1990) Maturation of pulmonary intravascular macrophage function in newborn lambs: Monastral blue retention. FASEB J 4(3):A577
Longworth KE, Steffey EP, Wolinet M et al. (1992) Comparative physiology of pulmonary intravascular macrophage function in ponies and calves. FASEB J 6(4):A1242
Palade GE (1988) The microvascular endothelium revisited. In: Simionescu N, Simionescu M (eds) Endothelial cell biology in health and disease. Plenum Press, New York, pp 1–22
Parkham MA, Mustard JF (1984) Normal and abnormal platelet activity. In: Laslo A (ed) Blood platelet function and medicinal chemistry. Elsevier Biomedical, New York, pp 61–128
Pozlansky MJ, Hutchinson SK, Davis PJ (1989) Enzyme replacement therapy in fibroblast from a patient with cholesteryl ester storage disease. Fed Proc 3:152–156
Simon GT (1981) Splenic Macrophages. In: Daems T (ed) The reticuloendothelial system. Plenum Press, New York, pp 486–495
Skutelsky YE, Hardy B (1976) Regeneration of plasmalemma properties in macrophages. Exp Cell Res 101:337–345
Sprague EA, Kelly JL, Suenram CA et al. (1985) Stimulation of albumin endocytosis by cationized ferritin in cultured aortic smooth muscle cells. Am J Pathol 121:433–443
Sprague EA, Edwards EH, Sueram CA et al. (1988) Low density lipoprotein endocytosis, influence of multivalent ligand cationized ferritin on acetylated low density lipoprotein endocytosis in cultured cells. Expt Mol Pathol 48:373–390
Staub NC (1989) Pulmonary vascular reactivity: a status report. In: Staub NC (ed) The pulmonary intravascular macrophage. Futura, New York, pp 123–139
Van Oss CJ (1978) Phagocytosis as a surface phenomenon. Ann Rev Microbiol 32:19–39
Warner AG, Brain JD (1990) The cell biology and pathogenic role of pulmonary intravascular macrophages. Am J Physiol: Lung Cell Mol Physiol 2:L1-L12
Warner AG, Barry BE, Brain JD (1986) Pulmonary intravascular macrophages in sheep. Morphology and function of a noval constituent of mononuclear phagocyte system. Lab Invest 55:276–288
Werk R, Dunker R, Fisher S (1984) Polycationic polypeptides: a possible model for penetration enhancing factor in the invasion of host cells by Toxoplasma gondii. J Gen Microbiol 130:927–933
White JG (1984) The ultrastructure and regulatory mechanisms of blood platelets. In: Laslo A (ed) Blood platelet function and medicinal chemistry. Elsevier Biomedical, New York, pp 15–59
Winkler G (1988) Pulmonary intravascular macrophages in domestic animal species. Review of structural and functional properties. Am J Anat 191:217–234
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Atwal, O.S. Pulmonary intravascular macrophages (PIMs) and cationised ferritin-induced phagocytosis of platelets: A morphological study. Comparative Haematology International 2, 179–185 (1992). https://doi.org/10.1007/BF00216092
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00216092