Skip to main content
Log in

Transport of material between the nucleus, the chromatoid body and the golgi complex in the early spermatids of the rat

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The movement and transport of material between intranuclear dense particles, the chromatoid body and the Golgi complex have been studied in early spermatids of the rat. The analyses involved observation of living accurately identified cells, time-lapse cinemicrography and electron microscopy.

The chromatoid body establishes transient contacts with intranuclear material during early spermiogenesis. The chromatoid body also makes contacts with the Golgi complex. It is suggested that the chromatoid body receives material from the nucleus during the postmeiotic period and participates in the early formation of the acrosomic system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Benda, C.: Neue Mitteilungen über die Entwicklung der Genitaldrüsen und die Metamorphose der Samenzellen (Histogenese der Spermatozoen). Verhandlungen der Berliner Physiologischen Gesellschaft. Arch. Anat. Physiol. 549–552 (1891)

  • Bounoure, L.: Recherches sur la lignée germinale chez la Grenouille rousse aux premiers stades du développment. Ann. Sci. Nat. 10e sér 17, 67–248 (1934)

    Google Scholar 

  • Christensen, A.K., Mason, N.R.: Comparative ability of seminiferous tubules and interstitial tissue of rat testis to synthesize androgens from progestoerone-4-14C in vitro. Endocrinology 76, 646–656 (1965)

    Google Scholar 

  • Comings, D.E., Okada, T.A.: The chromatoid body in mouse spermatogenesis: Evidence that it may be formed by the extrusion of nucleolar components. J. Ultrastruct. Res. 39, 15–23 (1972)

    Google Scholar 

  • Daoust, R., Clermont, Y.: Distribution of nucleic acids in germ cells during the cycle of the seminiferous epithelium in the rat. Amer. J. Anat. 96, 255–283 (1955)

    Google Scholar 

  • Davis, J.R., Firlit, C.F.: Effect of glucose on uptake 1-lysine-H3 in cells of the seminiferous epithelium. Amer. J. Physiol. 209, 425–432 (1965)

    Google Scholar 

  • Eddy, E.M.: Cytochemical observations on the chromatoid body of male germ cells. Biol. Reprod. 2, 114–128 (1970)

    Google Scholar 

  • Eddy, E.M.: Fine structural observations on the form and distribution of nuage in germ cells of the rat. Anat. Rec. 178, 731–757 (1974)

    Google Scholar 

  • Fawcett, D.W., Eddy, E.M., Phillips, D.M.: Observations on the fine structure and relationships of the chromatoid body in mammalian spermatogenesis. Biol. Reprod. 2, 129–153 (1970)

    Google Scholar 

  • Hegner, R.W.: Experiments with chrysomelid beetles. III. The effects of killing parts of the eggs of Leptinotarsa decemlineata. Biol. Bull. 20, 237–251 (1911)

    Google Scholar 

  • Leblond, C.P., Clermont, Y.: Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann. N.Y. Acad. Sci. 55, 548–573 (1952)

    Google Scholar 

  • Loir, M.: Métabolisme de l'acide ribonucléique et des protéines dans les spermatocytes et les spermatids du Bélier (Ovis aries). Ann. Biol. Anim. Biochim. Biophys. 12, 203–219 (1972)

    Google Scholar 

  • Mahowald, A.P.: Polar granules in drosophila. II. Ultrastructural changes during early embryogenesis. J. exp. Zool. 167, 237–268 (1968)

    Google Scholar 

  • Monesi, V.: Ribonucleic acid synthesis during mitosis and meiosis in the mouse testis. J. Cell Biol. 22, 521–532 (1964)

    Google Scholar 

  • Monesi, V.: Synthetic activities during spermatogenesis in the mouse. RNA and protein. Exp. Cell Res. 39, 197–224 (1965)

    Google Scholar 

  • Monesi, V.: Chromosome activities during meiosis and spermiogenesis. J. Reprod. Fertil., Suppl. 13, 1–14 (1971)

    Google Scholar 

  • Parvinen, M., Jokelainen, P.T.: Rapid movements of the chromatoid body in the living early spermatids of the rat. Biol. Reprod. 11, 85–92 (1974)

    Google Scholar 

  • Parvinen, M., Vanha-Perttula, T.: Identification and enzyme quantitation of the stages of the seminiferous epithelial wave in the rat. Anat. Rec. 174, 435–450 (1972)

    Google Scholar 

  • Regaud, Cl.: Etudes sur la structure des tubes séminifères et sur la spermatogénèse chez les Mammifères. Arch. Anat. micr. Morph. exp. 11, 291–431 (1910)

    Google Scholar 

  • Söderström, K.-O., Parvinen, M.: Characterization of RNA synthesis in different stages of rat seminiferous epithelial cycle. Adaptation of new transillumination based cell separation methods. Mol. Cell. Endocrinol. (accepted for publication)

  • Stefanini, M., deMartino, C., Agostino, A.D., Agrestini, A., Monesi, V.: Nucleolar activity of rat primary spermatocytes. Exp. Cell Res. 86, 166–170 (1974)

    Google Scholar 

  • Sud, B.N.: The chromatoid body in spermatogenesis. Quart. J. micr. Sci. 102, 273–292 (1961a)

    Google Scholar 

  • Sud, B.N.: Morphological and histochemical studies of the chromatoid body and related elements in the spermatogenesis of the rat. Quart. J. micr. Sci. 102, 495–505 (1961b)

    Google Scholar 

  • Susi, F.R., Clermont, Y.: Fine structural modifications of the rat chromatoid body during spermatogenesis. Amer. J. Anat. 129, 177–192 (1970)

    Google Scholar 

  • Weakley, B.S.: Basic protein and ribonucleic acid in the cytoplasm of the ovarian oocyte in the golden hamster. Z. Zellforsch. 101, 69–84 (1971)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This work was supported by the Finnish National Research Council for Medical Sciences. The authors are grateful to Mrs. Marita Aaltonen and Mrs. Raija Andersen for their skilful technical assistance

Rights and permissions

Reprints and permissions

About this article

Cite this article

Söderström, KO., Parvinen, M. Transport of material between the nucleus, the chromatoid body and the golgi complex in the early spermatids of the rat. Cell Tissue Res. 168, 335–342 (1976). https://doi.org/10.1007/BF00215311

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00215311

Key words

Navigation