Summary
A substance immunologically related to mammalian insulin was demonstrated by immunofluorescence in one of five types of neurosecretory cells in the protocerebrum of the European corn borer, Ostrinia nubilalis. This A2 cell type contains a secretory material stainable with paraldehyde fuchsin.
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References
Beck SD, Hanec W (1960) Diapause in the European corn borer, Pyrausta nubilalis (Hübn). J Insect Physiol 4:304–318
Cloutier JE, Beck SD (1963) Spermatogenesis and diapause in the European corn borer, Ostrinia nubilalis. Ann Entomol Soc Am 56:253–255
Coons A, Leduc EH, Connolly JM (1955) Studies on antibody production. I. Method for histochemical demonstration of specific antibody and of application to the study of the hyperimmune rabbit. J Exp Med 102:49–59
Duve H, Thorpe A (1979) Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphora vomitoria (Diptera). Cell Tissue Res 200:187–191
Gahukar RT (1975) Nouvelles techniques adoptées pour l'élevage d'Ostrinia nubilalis Hübner sur milieu artificiel. Ann Zool Ecol A7:491–498
Ganagarajah M, Saleuddin SM (1970) A simple manoeuvre to prevent loss of secretion for performic acid — Victoria blue technique and a comparison of various neurosecretory stains. Can J Zool 48:1457–1458
Kramer KJ, Child CN, Spiers RD, Jacobs RM (1982) Purification of insulin-like peptides from insect haemolymph and royal jelly. Insect Biochem 12:91–98
Lavenseau L, Trabelsi M, Gadenne C, Girardie A (1983) Insulin-like substance and development of the European corn borer. Abstr Second Int CNRS Symp. Strasbour
Lavenseau L, Gadenne C, Trabelsi M (1984) Histological and ultrastructural study of the protocerebral neurosecretory cells of the European corn borer (in press)
Panov AA (1976) The composition of the medial neurosecretory cells of the pars intercerebralis in Calliphora and Lucilia adults. Zool Anz Jena 196:23–27
Teller JK, Rosinski G, Pilc L, Kasprzyk A, Lesicki A (1983) The presence of insulin-like hormone in heads and midguts of Tenebrio molitor L. (Coleoptera) larvae. Comp Biochem Physiol 74:463–465
Yui R, Fujita T, Ito S (1980) Insulin-gastrin-pancreatic-polypeptide-like immunoreactive neurons in the brain of the silkworm, Bombyx mori. Biomed Res 1:42–46
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E.R.A. CNRS n° 850
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Lavenseau, L., Gadenne, C. & Trabelsi, M. Immunofluorescent localization of a substance immunologically related to insulin in the protocerebral neurosecretory cells of the European corn borer. Cell Tissue Res. 238, 207–208 (1984). https://doi.org/10.1007/BF00215164
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DOI: https://doi.org/10.1007/BF00215164