Summary
Prekeratinized fetal epidermis may function as an osmoregulatory organ. This study shows that the structural response of fetal epidermis to cAMP (second messenger of antidiuretic hormones) is very similar to that in well characterized osmoregulatory epithelia. cAMP stimulation of unkeratinized fetal epidermis induces the appearance of “ aggregates” of intramembranous particles. These aggregates are present in the apical plasma membrane of the periderm or outermost cell layer of the epidermis. Tubular vesicles that contain the presumptive aggregates fuse with the apical plasma membrane during cAMP stimulation. The same response to cAMP and to antidiuretic hormones has been reported in the osmoregulatory ion- and water-transporting epithelia of amphibian urinary bladder and mammalian collecting ducts. In these systems aggregates have been positively correlated with water transport. Thus, the fetal epidermis may control and regulate its water permeability.
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References
Abramovich DR, Page KR (1972) Pathways of water exchange in the fetoplacental unit at mid pregnancy. J Obstet Gynaec Brit Cwlth 79:1099–1102
Bourguet J, Chevalier J, Hugon JS (1976) Alterations in membraneassociated particle distribution during antidiuretic challenge in frog urinary bladder epithelium. Biophys J 16:627–639
Breathnach AS (1971) Embryology of human skin. J Invest Derm 57:133–143
Brown D, Grosso KA, DeSousa RK (1983) Correlation between water flow and intramembrane particle aggregation in toad epidermis. Am J Physiol 245:C334-C342
Chevalier J, Bourguet J, Hugon JS (1974a) Membrane associated particles. Distribution in frog urinary bladder epithelium at rest and after oxytocin treatment. Cell Tissue Res 152:129–140
Chevalier J, Ripoche P, Pisam M, Bourguet J, Hugon JS (1974b) A time course study of water permeability and morphological alterations induced by mucosal hyperosmolarity in frog urinary bladder. Cell Tissue Res 154:345–356
Chevalier J, Adragna N, Bourguet J, Gobin R (1981) Fine structure of intramembranous particle aggregates in ADH-treated frog urinary bladder and skin: influence of glutaraldehyde and Nethyl maleimide. Cell Tissue Res 218:595–606
Dratwa M, Tisher CC, Sommer JR, Croker BP (1979) Intramembranous particle aggregation in toad urinary bladder after vasopressin stimulation. Lab Invest 40:46–54
Elias PM, McNutt NS, Friend DS (1977) Membrane alterations during cornification of mammalian squamous epithelia: a freeze fracture, tracer, and thin-section study. Anat Rec 189:577–594
France VM (1976) Active sodium uptake by the skin of foetal sheep and pigs. J Physiol 258:377–392
Harmanci MC, Kachadorian WA, Valtin H, DiScala VA (1978) Antidiuretic hormone-induced intramembranous alterations in mammalian collecting ducts. Am J Physiol 235:F440-F443
Harmanci MC, Stern P, Kachadorian WA, Valtin H, DiScala VA (1980) Vasopressin and collecting duct intramembranous particle clusters: a dose-response relationship. Am J Physiol 239:F560-F564
Hays RM (1983) Alteration of luminal membrane structure by antidiuretic hormone. Am J Physiol 245:C289-C296
Holbrook KA, Odland GF (1975) The fine structure of developing human epidermis: light, scanning, and transmission electron microscopy of the periderm. J Invest Derm 65:16–38
Holt WF, Perks AM (1977) The influence of vasopressin on the passage of tritiated water through the isolated amniotic membrane and other tissues from the fetal guinea pig. Can J Zool 55:1393–1403
Humbert F, Montesano R, Grosso A, deSousa RC, Orci L (1977) Particle aggregates in plasma and intracellular membranes of toad bladder (granular cell). Experientia 33:1364–1367
Kachadorian WA, Wade JB, DiScala VA (1975) Vasopressin-induced structural change in toad bladder luminal membrane. Science 190:67–69
Kachadorian WA, Wade JB, Uiterwyk CC, DiScala VA (1977) Membrane structural and functional responses to vasopressin in toad bladder. J Membr Biol 30:381–401
Lacy ER (1982) Marked reduction in intramembranous particle clusters in the terminal portion of inner medullary collecting ducts of antidiuretic rats. Cell Tissue Res 221:583–595
Lind T, Kendal A, Hytten FE (1972) The role of the fetus in the formation of amniotic fluid. J Obstet Gynaec Brit Cwlth 79:289–298
Müller J, Kachadorian WA, DiScala VA (1980) Evidence that ADH-stimulated intramembrane particle aggregates are transferred from cytoplasmic to luminal membranes in toad bladder epithelial cells. J Cell Biol 85:83–95
Parmley TH, Seeds AE (1970) Fetal skin permeability to isotopic water (THO) in early pregnancy. Am J Obstet Gynec 108:128–131
Riddle CV (1984) Structural changes of the zonula occludens in fetal epidermis during development. Anat Rec 208:147a
Roth J, LeRoith D, Shiloach J, Rosenzweig JL, Lesniak MA, Havrankova J (1982) The evolutionary origins of hormones, neurotransmitters, and other extracellular chemical messengers. N Engl J Med 306:523–527
Vizsolyi E, Perks AM (1969) New neurohypophysial principle in foetal mammals. Nature 223:1169–1170
Wade JB (1978) Membrane structural specialization of the toad urinary bladder revealed by the freeze-fracture technique. III. Location, structure and vasopressin dependence of intramembrane particle arrays. J Membr Biol 40:281–296
Wade JB (1980) Hormonal modulation of epithelial structures. Curr Topics Membr Transport 13:123–147
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Riddle, C.V. Intramembranous response to cAMP in fetal epidermis. Cell Tissue Res. 241, 687–689 (1985). https://doi.org/10.1007/BF00214593
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DOI: https://doi.org/10.1007/BF00214593