Summary
The content of visual pigment in one eye of Calliphora ‘chalky’ was measured spectrophotometrically and related to the ultrastructure of the other eye and the density of membrane particles as revealed by the freeze-fracture technique. Electron microscopy of thin-sectioned material showed that the manifestations of synthesis and breakdown of photoreceptor membrane were most prominent in flies kept in blue light, in comparison to flies kept in green light or darkness, in which only a moderate breakdown of membrane was evident.
By subjecting flies to different light regimes it was found that the density of membrane particles was related to the content of visual pigment, but not on a one-to-one basis. In particular, the particle density in flies with a low (< 10%) rhodopsin content, produced by raising flies on a vitamin A-deficient diet or by exposure to green light, was still about 35% of that of normal flies. Taken together the results indicate that all rhodopsin molecules are particles but that some particles represent another protein that most probably serves to maintain the structural integrity of the photoreceptor membrane. Furthermore, membrane synthesis can take place in the absence of rhodopsin synthesis.
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References
Blest AD, Williams DS, Kao L (1980) The posterior median eyes of the dinopid spider Menneus. Cell Tissue Res 211:391–403
Boschek CB, Hamdorf K (1976) Rhodopsin particles in the photoreceptor membrane of an insect. Z Naturforsch 39c: 762
Brown PK, Schwemer J (1977) Insect visual pigments. Invest Ophthalmol Visual Sci 16 (Suppl): 93–94
Chi C, Carlson SD (1979) Ordered membrane particles in rhabdomeric microvilli of the housefly (Musca domestica L.). J Morphol 161:309–322
Hamdorf K (1979) The physiology of invertebrate visual pigments. In: Autrum H (ed) Vision in invertebrates, Handbook sens physiol, vol VII/6A, Springer, Berlin Heidelberg New York, pp 145–224
Hamdorf K, Schwemer J (1975) Photoregeneration and the adaptation process in insect photoreceptors. In: Snyder AW, Menzel R (eds) Photoreceptor optics, Springer, Berlin Heidelberg New York, pp 263–289
Harris WA, Ready DF, Lipson ED, Hudspeth AJ, Stark WS (1977) Vitamin A deprivation and Drosophila photopigments. Nature (Lond) 266:648–650
Larrivee DC, Conrad SK, Stephenson RS, Pak WL (1981) Mutation that selectively affects rhodopsin concentration in the peripheral photoreceptors of Drosophila melanogaster. J Gen Physiol 78:521–545
Paulsen R, Schwemer J (1979) Vitamin A deficiency reduces the concentration of visual pigment protein within blowfly photoreceptor membranes. Biochim Biophys Acta 557:385–390
Paulsen R, Schwemer J (1983) Biogenesis of blowfly photoreceptor membranes is regulated by 11-cis retinal. Eur J Biochem 137:609–614
Schinz RH, Lo MC, Larrivee DC, Pak WL (1982) Freeze-fracture study of the Drosophila photoreceptor membrane: mutations affecting membrane particle density. J Cell Biol 93:961–969
Schwemer J (1983) Pathways of visual pigment regeneration in fly photoreceptor cells. Biophys Struct Mech 9:287–298
Schwemer J (1984) Renewal of visual pigment in photoreceptors of the blowfly. J Comp Physiol (in press)
Stavenga DG, Schwemer J (1983) Visual pigment in invertebrates. In: Ali MA (ed) Photoreception and vision in invertebrates. Plenum, New York London (in press)
White RH, Lord E (1975) Diminuation and enlargement of the mosquito rhabdom in light and darkness. J Gen Physiol 65:583–598
White RH, Gifford D, Michand NA (1980) Turnover of photoreceptor membrane in the larval mosquito ocellus: rhabdomeric coated vesicles and organelles of the vacuolar system. In: Williams TP, Baker BN (eds) The effects of constant light on visual processes. Plenum, New York London, pp 271–296
Williams DS (1980) Organisation of the compound eye of the tipulid fly during the day and night. Zoomorphologie 95:85–104
Williams DS (1982a) Ommatidial structure in relation to turnover of photoreceptor membrane in the locust. Cell Tissue Res 225:595–617
Williams DS (1982b) Rhabdom size and photoreceptor membrane turnover in a muscoid fly. Cell Tissue Res 226:629–639
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Schwemer, J., Henning, U. Morphological correlates of visual pigment turnover in photoreceptors of the fly, Calliphora erythrocephala . Cell Tissue Res. 236, 293–303 (1984). https://doi.org/10.1007/BF00214230
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DOI: https://doi.org/10.1007/BF00214230