Summary
The secretion of the subcommissural organ (SCO) of the rat was studied by means of immunocytochemistry at the electron-microscopic level with the use of (1) the polar embedding medium Lowicryl K4M at -30° C, (2) the protein A-gold technique, and (3) a rabbit antiserum against bovine Reissner's fiber (see Sterba et al. 1981).
Two different substructures of the ependymal and the hypendymal SCO-cells display a positive immunocytochemical reaction: (1) sacs containing flocculent secretion, which originate from the granular endoplasmic reticulum, and (2) vacuoles filled with fine granular secretion, which are pinched off from the Golgi apparatus. The secretory material of the sacs and the vacuoles is discharged both (i) apically into the cerebrospinal fluid and (ii) basally into intercellular spaces of the SCO-hypendyma. The apically released secretion is condensed to a lamina-like formation, which more caudally assumes the form of Reissner's fiber. The route of the basally released secretion remains, however, vague. The “periodically striated bodies”, which were thought to be morphological mediators of the discharge of the secretion into the capillaries, are never labeled by gold particles.
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References
Bomhard K von, Köhl W, Schinko I, Wetzstein R (1974) Feinbau und Passageverhalten der Capillaren im Subcomissuralorgan der Ratte. Z Anat Entwickl Gesch 144:101–122
Carlemalm E, Garavito RM, Villiger W (1982) Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc 126:123–143
Craig S, Goodchild DJ (1982) Postembedding immunolabelling. Some effects of tissue preparation on the antigenicity of plant proteins. Eur J Biol 28:251–256
Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold solutions. Nature Phys Sci 241:20–22
Fryer PR, Wells C, Ratcliffe A (1983) Technical difficulties overcome in the use of Lowicryl 4KM EM resin. Histochemistry 77:141–143
Grube D (1980) Non-specific binding of immunoglobulins to Gcells by ionic interactions. Histochemistry 66:149–167
Herrlinger H (1970) Licht- und elektronenmikroskopische Untersuchungen am Subcommissuralorgan der Maus. Adv Anat Embryol Cell Biol 42: 1–73
Hofer H, Meinel W, Erhardt H (1980) Electron microscopic study of the origin and formation of Reissner's fiber in the subcommissural organ of Cebus apella (Primates, Platyrrhini). Cell Tissue Res 205:295–301
Kellenberger E, Carlemalm E, Villiger W, Roth J, Garavito RM (1980) Low denaturation embedding for electron microscopy of thin sections. Chemische Werke Lowi GmbH, Waldkraiburg, FRG, 1–59
Kimble JE, Møllgård K (1973) Evidence for basal secretion in the subcommissural organ of the adult rabbit. Z Zellforsch 142:223–239
Krstić R (1973) Ultrastrukturelle Lokalisation von Mukosubstanzen der Zellhülle im Subcommissuralorgan der Ratte. Z Zellforsch 139:237–252
Leonhardt H (1980) Ependym und Circumventriculäre Organe. In: Oksche A, Vollrath L (eds) Handbuch der mikroskopischen Anatomie des Menschen Band IV, 10. Teil Neuroglia I. Springer, Heidelberg Berlin New York, pp 177–665
Lösecke W, Naumann W, Sterba G (1982) Immuncytochemische Darstellung von Neuropeptiden (Neurophysin und Oxytocin) mit der Protein-A-Gold-Technik. Acta Histochem 71:201–208
Müller H (1973) Bildung, Transport und Ausleitung des Sekretes im Subkommissuralorgan niederer Wirbeltiere. Wiss Z Karl Marx Univ Leipzig, Mat Naturwiss R 22:337–379
Murakami M, Tanizaki T (1963) An electron microscopic study on the toad subcommissural organ. Arch Histol Jpn 23:337–358
Murakami M, Okita S, Nagano Y (1970) Electron microscopic observation of the subcommissural organ in the soft-shelled turtle, Amyda japonica. Arch Histol Jpn 31:199–208
Murakami M, Shimada T, Oribe T, Hiraki T (1972) An electron microscopic study on the subcommissural organ of the monkey, Macacus fuscatus. Arch Histol Jpn 34:61–72
Oksche A (1969) The subcommissural organ. J Neuro-Visc Relat (Suppl) 9:111–139
Papacharalampous N, Schwink A, Wetzstein R (1968) Elektronenmikroskopische Untersuchungen am Subcommissuralorgan des Meerschweinchens. Z Zellforsch 90:202–229
Roth J (1982) The protein A-gold (pAg) technique. A qualitative and quantitative approach for antigen localization on thin sections. In: Bullock GR, Petrusz P (eds) Techniques in immunocytochemistry. Academic Press, New York London, pp 107–133
Roth J, Bendayan M, Orci L (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem 26:1074–1084
Roth J, Bendayan M, Carlemalm E, Villiger W, Garavito RM (1981) Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem 29:663–671
Stanka P, Schwink A, Wetzstein R (1964) Elektronenmikroskopische Untersuchung des Subcommissuralorgans der Ratte. Z Zellforsch: 277–301
Sterba G, Kleim I, Naumann W, Petter H (1981) Immunocytochemical investigation of the subcommissural organ in the rat. Cell Tissue Res 218:659–662
Sterba G, Kiessig Chr, Kleim I, Naumann W, Petter H (1982a) Immunzytochemische Untersuchungen an den Subkommissuralorganen von Rind, Ziege und Schaf. Biol Zbl 101:241–248
Sterba G, Kiessig Chr, Naumann W, Petter H, Kleim I (1982b) The secretion of the subcommissural organ. A comparative immunocytochemical investigation. Cell Tissue Res 226:427–439
Talanti S (1966) The subcommissural organ of the reindeer (Rangifer tarandus) with reference to secretory phenomena. Anat Anz 119:99–103
Wetzstein R, Schwink A, Stanka P (1963) Die periodisch strukturierten Körper im Subcommissuralorgan der Ratte. Z Zellforsch 61:493–523
Wolf G, Sterba G (1972) Zur stofflichen Charakteristik des Reissnerschen Fadens. Acta Zool (Stockholm) 53:147–154
Zsigmondy R (1905) Zur Erkenntnis der Kolloide. Über inversible Hydrosole und Ultramikroskopie. Fischer, Jena
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Supported by grants from the Ministry for Science and Technology of the German Democratic Republic
The expert technical assistance of Mrs. B. Wolff, Mrs. S. Mehnert, Mrs. E. Siebert, Mrs. Ch. Schneider, and Mrs. I. Seifert is gratefully acknowledged
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Lösecke, W., Naumann, W. & Sterba, G. Preparation and discharge of secretion in the subcommissural organ of the rat. Cell Tissue Res. 235, 201–206 (1984). https://doi.org/10.1007/BF00213741
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DOI: https://doi.org/10.1007/BF00213741