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A qualitative in vitro evaluation of the degradable materials poly(caprolactone), poly(hydroxybutyrate) and a poly(hydroxybutyrate)-(hydroxyvalerate) copolymer

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Abstract

A qualitative in vitro evaluation of poly(caprolactone) (PCL), poly(hydroxybutyrate) (PHB) and a poly(hydroxybutyrate)-(hydroxyvalerate) (PHB-PHV) copolymer was carried out using primary human osteoblasts (HOB) and a human osteosarcoma (HOS) cell line. The cells were grown on films of these polymers and cultured for 2 and 4 days with cells grown on Thermanox as a control. The cells on each of the polymers exhibited different cellular morphologies with different rates of cell proliferation. Results from a preliminary degradation study demonstrated that biodegradable materials can be partially degraded using enzymes such as papain and trypsin. Of the solutions tested, papain caused the greatest degradation, with phosphate-buffered saline (PBS) a physiological buffer having very little effect over a six week period. The bone cells were grown on partially degraded polymers and no differences in the performance of HOS and HOB cells on the materials were observed.

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Authors and Affiliations

  1. Institute of Orthopaedics, RNOHT, Brockley Hill, HA7 4LP, Stanmore, Middlesex, UK

    N. Gurav & S. Downes

  2. IRC in Biomedical Materials, RNOHT, Brockley Hill, HA7 4LP, Stanmore, Middlesex, UK

    N. Gurav & S. Downes

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  1. N. Gurav
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  2. S. Downes
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Gurav, N., Downes, S. A qualitative in vitro evaluation of the degradable materials poly(caprolactone), poly(hydroxybutyrate) and a poly(hydroxybutyrate)-(hydroxyvalerate) copolymer. J Mater Sci: Mater Med 5, 784–787 (1994). https://doi.org/10.1007/BF00213135

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