Summary
The nasal cavities of opossums prior to and shortly after birth were examined by scanning electron microscopy. Numerous morphologically mature olfactory receptor neurons are observed in the dorso-rostralmost extent of the olfactory epithelium positioned adjacent to the opening of the nares in all prenatal stages and newborn animals examined. The remainder of the olfactory epithelium, occupying a more dorso-caudal position within the nasal cavity, is undifferentiated, and lacks morphologically mature receptor neurons. A short transition zone of stratified squamous epithelium lies between the epithelium lining the nares and olfactory epithelium. It forms an abrupt junction with the latter. The remainder of the nasal cavity in this group of animals is lined by a non-ciliated pseudostratified type (undifferentiated respiratory) of epithelium. By the end of the second postnatal week the morphologically mature olfactory epithelium is no longer observed in the vestibular area of the nasal cavity, which is lined by stratified squamous epithelium at this time. Mature receptor neurons are now observed within the olfactory epithelium lining the roof of the nasal cavity and covering the turbinates. The greater part of the nasal cavity is lined by a ciliated respiratory epithelium. It is proposed that the precocious differentiation of mature olfactory receptor neurons within the rostral-most extent of the olfactory epithelium just prior to birth is important in guiding the newborn young to the pouch.
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References
Breipohl W, Ohyama M (1981) Comparative and developmental SEM studies on olfactory epithelia in vertebrates. (Biomedical aspects and speculations). Biomed Res 2 [Suppl]: 437–448
Cannon JR, Bakker HR, Bradshaw S, McDonald IR (1976) Gravity as the sole navigational aid to the newborn quokka. Nature 259:42
Cuschieri A, Bannister LH (1975a) The development of the olfactory mucosa jn the mouse: light microscopy. J Anat 119:277–286
Cuschieri A, Bannister LH (1975b) The development of the olfactory mucosa in the mouse: electron microscopy. J Anat 119:471–498
Distel H, Hudson R (1985) The contribution of the olfactory and tactile modalities to the nipple-search behavior of newborn rabbits. J Comp Physiol [A] 157:599–605
Farbman AI, Margolis FL (1980) Olfactory marker protein during ontogeny: Immunohistochemical localization. Dev Biol 74:205–215
Farbman AI, Menco BPhM (1986) Development of olfactory epithelium in the rat. In: Breipohl W (ed) Ontogeny of olfaction. Springer, Berlin Heidelberg New York, pp 45–56
Gemmell RT, Nelson J (1988) Ultrastructure of the olfactory system of three newborn marsupial species. Anat Rec 221:655–662
Gemmell RT, Nelson J (1989) Vestibular system of the newborn marsupial cat Dasyurus hallucatus. Anat Rec 225:203–208
Gemmell RT, Peters B, Nelson J (1988) Ultrastructural identification of Merkel cells around the mouth of the newborn marsupial. Anat Embryol 177:403–408
Gesteland RC, Yancy RA, Farbman AI (1982a) Development of olfactory receptor cell axons. Dev Brain Res 19:205–213
Gesteland RC, Yancy RA, Farbman AI (1982b) Development of olfactory receptor neuron selectivity in rat fetus. Neuroscience 7:3127–3136
Graziadei PPC, Graziadei GAM, Stanley RS (1979) Distribution of the olfactory marker protein in the olfactory mucosa of pre-and post-natal mice. Proc Soc Neurosci 5:404
Hartman CG (1920) Studies in the development of the opossum Didelphis virginiana. V. The phenomena of parturition. Anat Rec 19:1–11
Hinds JW, Hinds PL (1976) Synapse formation in the mouse olfactory bulb. 2. Morphogenesis. J Comp Neurol 160:41–62
Hudson R, Distel H (1983) Nipple location by newborn rabbits: Evidence for pheromonal guidance. Behaviour 81:260–275
Hudson R, Distel H (1984) Nipple-search pheromone in rabbits: Dependence on season and reproductive state. J Comp Physiol [A] 155:13–17
Jones TE, Munger BL (1985) Early differentiation of the afferent nervous system in glabrous snout skin of the opossum (Monodelphis domesticus). Somatosens Res 3:169–184
Krause WJ, Leeson CR (1973) The postnatal development of the respiratory system of the opossum. I. Light and scanning electron microscopy. Am J Anat 137:337–356
Langworthy OR (1928) The behaviour of pouch young opossums correlated with myelinization of tracts in the nervous system. J Comp Neurol 46:201–240
Larsell O, McCrady E, Zimmerman AA (1935) Morphological and functional development of the membranous labyrinth in the opossum. J Comp Neurol 63:95–118
Lin PJJ, Phelix C, Krause WJ (1988) An immunohistochemical study of olfactory epithelium in the opossum before and after birth. Z Mikrosk Anat Forsch 102:272–282
Loo YT (1930) The forebrain of the opossum, Didelphis virginiana. Part I. Gross anatomy. J Comp Neurol 51:13–64
Loo YT (1931) The forebrain of the opossum, Didelphis virginiana. Part II. Histology. J Comp Neurol 52:1–148
Margolis FL (1980) A marker protein for the olfactory chemoreceptor neuron. In: Bradshaw RA, Schneider DM (eds) Proteins of the nervous system. 2nd edn. Raven Press, New York, pp 59–84
McCrady E (1938) The embryology of the opossum. Am Anat Memoirs No. 16, Wistar Institute, Philadelphia
Menco BPhM (1980a) Qualitative and quantitative freeze-fracture studies on olfactory and nasal respiratory structures of frog, ox, rat, and dog. I. A general survey. Cell Tissue Res 207:183–209
Menco BPhM (1980b) Qualitative and quantitative freeze-fracture studies on olfactory and nasal respiratory epithelial surfaces of frog, ox, rat and dog. II. Cell apices, cilia, and microvilli. Cell Tissue Res 211:5–30
Menco BPhM (1988) Pre-natal development of rat nasal epithelia IV. Freeze-fracturing on apices, microvilli and primary and secondary cilia on olfactory and respiratory epithelial cells, and on olfactory axons. Anat Embryol 178:309–326
Menco BPhM, Farbman AI (1985) Genesis of cilia and microvilli of rat nasal epithelia during pre-natal development. I. Olfactory epithelium, qualitative studies. J Cell Sci 78:283–310
Menco BPhM, Farbman AI (1987) Genesis of cilia and microvilli of rat nasal epithelia during pre-natal development. III. Respiratory epithelium surface, including a comparison with the surface of the olfactory epithelium. J Anat 152:145–160
Morest DK (1970) A study of neurogenesis in the forebrain of opossum pouch young. Z Anat Entwicklungsgesch 130:265–305
Nakashima T, Kimmelman CP, Snow JB (1984) Structure of human fetal and adult olfactory neuroepithelium. Arch Otolaryngol 110:614–646
Rose M (1926) Der Allocortex bei Tier und Mensch. Teil I. J PsycholNeurol34:1–111
Scinfert K (1970) Die Ultrastruktur des Riechepithels beim Makros metiker. Eine elektronenmikroskopische Untersuchung. In: Bargmann W, Doerr W (eds) Normale und pathologische Anatomie. Monographien in zwangloser Folge. Thieme, Stuttgart, pp 1–99
Teicher MH, Blass EM (1976) Suckling in newborn rats: eliminated by nipple lavage, reinstated by pup saliva. Science 193:422–425
Teicher MH, Blass EM (1977) First suckling response to the newborn albino rat: the roles of olfaction and amniotic fluid. Science 198:635–636
Ulinski PS (1971) External morphology of pouch young opossum brains: a profile of opossum neurogenesis. J Comp Neurol 142:33–58
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Currently Visiting Professor to The Department of Anatomy and Human Biology, The University of Western Australia, Perth, Australia
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Krause, W.J. A scanning electron microscopic study of the opossum nasal cavity prior to and shortly after birth. Anat Embryol 185, 281–289 (1992). https://doi.org/10.1007/BF00211827
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DOI: https://doi.org/10.1007/BF00211827