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Gene responsible for dificient activity of the β subunit of C8, the eighth component of complement, is located on mouse chromosome 4

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Abstract

Sera from 73 strains of mice were tested for hemolytic activity through the classical and the alternative pathways (CP and AP) in a single radical hemolysis assay. Sera from 16 out of 45 laboratory inbred strains had n o lytic activity, and Ouchternoly analysis with anti-C5 serum showed them to be C5-deficient. Sera from 2 out of 28 strains derived from wild mice also had no lytic activity, but the C5 molecule was detectable in both. The hemolytic activity of sera from these strains can be restored serum deficient in C8α-γ, leading us to conclude that strains M.MOL-MSM (MSM) and Mae are deficient in the β subunit of C8. Typing of (DBA/2J ×MSM)F1 hybrids and of progeny of a backcross to MSM showed that this C8 deficiency is controlled by a singles recessive gene, designated C8b; the allele with hemolytic activity is C8b 1; and the allele with no activity C8b 0. Because of synteny homologies in mouse and human , we looked for and found close linkage between C8b and Pgm-2. Typing of recombinant mice for Mup-1 mapped the C8b locus 2.3 centimorgans (cM) telomeric to Pgm-2 on mouse chromosome 4.

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Authors and Affiliations

  1. Departments of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

    Shinobu Tanaka, Mitsuru Sakaizumi & Hiromichi Yonekawa

  2. Laboratory Animal Science and Medical Chemistry, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

    Takakazu Suzuki

  3. Department of Cytogenetics, National Institute of Genetics, Shizuoka, Japan

    Yoshinobu Harada & Kazuo Moriwaki

  4. Genetic Stock Research Center, National Institute of Genetics, Shizouka, Japan

    Nobumoto Miyashita

  5. Department of Laboratory Animal Science, Ohu University of Dentistry, Fukushima, Japan

    Yoshibumi Matsushima

  6. Department of Research, Osaka Red Cross Blood Center, Osaka, Japan

    Yasuo Fukumori

  7. Department of Clinical Pathology, Osaka Medical College, Osaka, Japan

    Shinya Inai

Authors
  1. Shinobu Tanaka
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  2. Takakazu Suzuki
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  3. Mitsuru Sakaizumi
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  4. Yoshinobu Harada
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  5. Yoshibumi Matsushima
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  6. Nobumoto Miyashita
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  7. Yasuo Fukumori
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  8. Shinya Inai
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  9. Kazuo Moriwaki
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  10. Hiromichi Yonekawa
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Tanaka, S., Suzuki, T., Sakaizumi, M. et al. Gene responsible for dificient activity of the β subunit of C8, the eighth component of complement, is located on mouse chromosome 4. Immunogenetics 33, 18–23 (1991). https://doi.org/10.1007/BF00211691

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  • DOI: https://doi.org/10.1007/BF00211691

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