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Antibody-enhanced phagocytosis of lamprey polymorphonuclear leucocytes against sheep erythrocytes

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Summary

Centrifugation on Ficoll-Paque of the blood from the Arctic lamprey, Lampetra japonica, yielded a uniform population of leucocytes containing about 90% polymorphonuclear leucocytes (PMNs). The PMNs showed a significantly enhanced phagocytic activity in vitro against sheep erythrocytes (ES), provided they were opsonized with the specific lamprey antibody (AL) against ES. This enhanced phagocytosis by PMN was more active at 4° C than 15° C, being dependent on Ca++; the opsonic activity of lamprey antibody was not replaced by bullfrog antibody. By the use of electron microscopy, it was found that the PMNs possess a well developed Golgi complex and rough ER, as well as highly variable cytoplasmic granules. Observations by both SEM and TEM revealed the formation of prominent pseudopodia, lamellipodia and phagosomes by the PMN during the process of engulfing erythrocytes.

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References

  • Avtalion RA, Shahrabani RS (1975) Studies on phagocytosis in fish. I. In vitro uptake and killing of living Staphylococcus aureus by peripheral leucocytes of carp (Cyprinus carpio). Immunology 29:1181–1187

    Google Scholar 

  • Fujii T, Murakawa S (1981) Immunity in lamprey. III. Occurrence of the complement-like activity. Develop Comp Immunol 5: in press

  • Fujii T, Nakagawa H, Murakawa S (1979a) Immunity in lamprey. I. Production of haemolytic and haemagglutinating antibody to sheep red blood cells in Japanese lampreys. Develop Comp Immunol 3:441–451

    Google Scholar 

  • Fujii T, Nakagawa H, Murakawa S (1979b) Immunity in lamprey. II. Antigen-binding responses to sheep erythrocytes and hapten in the ammocoete. Develop Comp Immunol 3:609–620

    Google Scholar 

  • Gérard P (1933) Sur le système arthrophagocytaire chez l'ammocète de la Lampetra planeri (Bloch). Archs Biol Paris 44:327–346

    Google Scholar 

  • Good RA, Finstad J, Litman GW (1972) Immunology. In: Hardisty MW, Potter IC (eds) The biology of lampreys Vol II. Academic Press London, p 405

    Google Scholar 

  • Jordan HE, Speidel CC (1930) Blood formation in cyclostomes. Am J Anat 46:355–391

    Google Scholar 

  • Kelényi G, Larsen LO (1976) The haematopoietic supraneural organ of adult, sexually immature river lampreys (Lampetra fluviatilis L. Gray) with particlular reference to azurophil leucocytes. Acta Biol Acad Sci Hung 27:45–56

    Google Scholar 

  • Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414

    Article  CAS  PubMed  Google Scholar 

  • Marchalonis JJ, Edelmann GM (1968) Phylogenetic origins of antibody structure. III. Antibodies in the primary immune response of the sea lamprey, Petromyzon marinus. J Exp Med 127:891–914

    Google Scholar 

  • Mayer MM (1961) Complement and complement fixation. In: Kabat EA, Mayer MM (eds) Experimental immunochemistry. Charles C Thomas Publisher Springfield Illinois, USA, p 133

    Google Scholar 

  • Nakao T (1978) An electron microscopic study of the cavernous bodies in the lamprey gill filament. Am J Anat 151:316–336

    Google Scholar 

  • Piavis GW, Hiatt JL (1971) Blood cell lineage in the sea lamprey, Petromyzon marinus (Pisces: Petromyzontidae). Copeia 1971:722–728

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–211

    Article  CAS  PubMed  Google Scholar 

  • Stossel TP (1974) Phagocytosis. N Engl J Med 290:717–723, 774–780, 833–839

    Google Scholar 

  • Yamaguchi K (1979) Electron microscopic study on the arterial system in lamprey gill, with special reference to the fine structure of cavernous body. Yamaguchi Igaku 28:257–269 (in Japanese with English summary)

    Google Scholar 

  • Yamaguchi K, Tomonaga S, Ihara K, Awaya K (1979) Electron microscopic study of phagocytic lining cells in the cavernous body of the lamprey gill. J Electron Microsc 28:106–116

    Google Scholar 

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Authors and Affiliations

  1. Zoological Institute, Faculty of Science, Hokkaido University, Sapporo, Japan

    Tamotsu Fujii

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  1. Tamotsu Fujii
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The author thanks Asst. Prof. Dr. Ch. Katagiri for his encouragement and advice throughout the course of this study and Dr. S. Tochinai for critical reading of this manuscript, and Mr. Y. Takakuwa for his technical assistance

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Fujii, T. Antibody-enhanced phagocytosis of lamprey polymorphonuclear leucocytes against sheep erythrocytes. Cell Tissue Res. 219, 41–51 (1981). https://doi.org/10.1007/BF00210017

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  • DOI: https://doi.org/10.1007/BF00210017

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