Abstract
Transgenic tobacco (Nicotiana tabacum L.), licorice (Glycyrrhiza uralensis Fisher) and foxglove (Digitalis purpurea L.) were obtained with binary vector systems based on a disarmed Agrobacterium tumefaciens and on a virulent A. rhizogenes. The chimeric neomycin phosphotransferase II (NPT-II) gene (kan) and the ß-glucuronidase (GUS) gene (uidA) were under the control of the TR1′ and 2′ promoters, respectively, on a binary vector, pGSGluc1. Tissue-specific expression of the chimeric TR2' -uidA gene was studied using regenerants of N. tabacum from transformed callus and hairy roots, and also using the transformed roots of G. uralensis and D. purpurea. In all these transformed tissues, the phloem and surrounding tissues were stained with 5-bromo-4-chloro-3-indolyl-ß-d-glucuronide, showing the specific expression of uidA. The enzymatic assays of NPT-II and GUS indicated that the expression of both TR1' -kan and TR2'- uidA were coordinately enhanced by wounding and by the addition of plant growth regulators. These results indicate that the gene expression by the dual TR promoters is regulated in the several plant species studied in a tissue-specific manner and enhanced by physiological stresses.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GUS:
-
β-glucuronidase
- Km = kanamycin:
-
NPT-II neomycin phosphotransferase II
- Rif:
-
rifampicin
- X-gluc:
-
5-bromo-4-chloro-3-indolyl-β-d-glucuronide
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We wish to thank Drs. J. Leemans and J. Van Rompaey (Plant Genetic Systems, Gent, Belgium) for providing pGSGluc1; Dr. A. Caplan (Laboratorium voor Genetica, Rijksuniversiteit Gent) for critical reading of the manuscript; Dr. L. Jouanin (INRA, Versailles, france) for a gift of pLJ1. Claforan™ was from Hoechst Japan (Tokyo, Japan). This research was supported, in part, by Grants-in-Aid from the Ministry of Education, Science and Culture, Japan, Special Cooperation Funds for Promoting Science and Technology Agency, Japan, and the Ministry of Health and Welfare, Science Research Fund Subsidy granted to the Japan Health Sciences Foundation. M.Y. is supported by JSPS Fellowships for Japanese Junior Scientists.
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Saito, K., Yamazaki, M., Kaneko, H. et al. Tissue-specific and stress-enhancing expression of the TR promoter for mannopine synthase in transgenic medicinal plants. Planta 184, 40–46 (1991). https://doi.org/10.1007/BF00208234
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DOI: https://doi.org/10.1007/BF00208234