Summary
Studies on altering the immunogenicity of isolated Meth A surface membranes are described. Balb/c mice, the species of origin of the transplantable Meth A methylcholanthrene-induced lymphosarcoma were immunized IP on days 0 and 1 with isolated Meth A membrane treated in various ways. The mice were challenged IP on day 4 with varying numbers of viable Meth A cells. A 2,500 Meth A cell challenge is uniformly lethal to Balb/c mice. Immunization with unaltered membranes protects 50%–70% of animals against a 50,000 cell challenge. Incorporation of membranes in Freund's complete adjuvant destroys the membranes' immunizing capacity. Pertussis vaccine increases membrane TSTA immunogenicity in an apparently nonspecific way, so that 80%–90% of the mice are protected against a 250,000 cell challenge. Membranes reacted with diazotized arsanilate, neuraminidase, tannic acid, bis-diazobenzidine, and heparin have diminished immunogenicity. Poly-l-lysine and certain lectins enhance immunogenicity. Concanavalin A is the most potent of the lectins. Membranes reacted with this substance protect 60%–70% of animals against a 600,000 cell challenge.
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McCollester, D.L. Immunogenicity alterations induced in isolated Meth A sarcoma surface membranes. Cancer Immunol Immunother 8, 249–256 (1980). https://doi.org/10.1007/BF00206043
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DOI: https://doi.org/10.1007/BF00206043