Summary
Killed Brucella abortus organisms of the vaccinal strain B19 were detoxified by incubation in NaOH. A 24-h incubation in 0.01 M NaOH increased the LD50 of smooth (S) and rough (R) organisms 2–3 times in normal mice and 50–100 times in adrenalectomized mice. This NaOH treatment did not alter the antitumor activity of Brucella abortus as shown in EL4 lymphoma- and Lewis carcinoma-grafted mice. After incubation in NaOH, S bacteria injected IV retained their ability to provoke spleen hypertrophy and antibody synthesis, and S and R organisms injected into he footpad had comparable ability to induce granulomas. NaOH treatment tended to diminish the mitogenic activity of R bacteria for spleen cell cultures.
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Le Garrec, Y., Martin, A., Collet, B. et al. Antitumor properties of chemically detoxified killed Brucella abortus organisms. Cancer Immunol Immunother 11, 63–68 (1981). https://doi.org/10.1007/BF00205776
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DOI: https://doi.org/10.1007/BF00205776