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Inhibition of normal allogeneic lymphocyte mitogenesis by a factor released from human tumor cells in culture

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Summary

Human tumor and normal cell lines in culture were examined for the release of factors capable of inhibiting lymphocyte blastogenesis. Supernatants from tumor cell cultures of melanoma, carcinoma (lung, colon, breast), sarcoma, and normal fibroblasts inhibited normal lymphocyte response to PHA. Only supernatants from the tumor cell lines C1 (colon carcinoma), 734B (breast carcinoma), and 231 (breast carcinoma) were found to inhibit both PHA and ConA responses significantly. The two breast carcinoma cell lines, 734B and 231, which also were capable of inhibiting lymphocyte responses to PPD and alloantigens, were investigated further. The inhibition of lymphocyte blastogenesis caused by the supernatant of these two cell lines could not be overcome by the addition of added mitogen. Further experiments showed that the inhibition was not due to nutrient deficiencies and the supernatants were not directly toxic to the lymphocyte cultures as judged by trypan blue exclusion.

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Authors and Affiliations

  1. Division of Oncology, Department of Surgery, UCLA School of Medicine, University of California, 90024, Los Angeles, CA

    Clifford M. Renk, Rishab K. Gupta & Donald L. Morton

  2. Surgical Service, Sepulveda Veterans Administration Hospital, 91343, Sepulveda, CA, USA

    Rishab K. Gupta & Donald L. Morton

Authors
  1. Clifford M. Renk
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  2. Rishab K. Gupta
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  3. Donald L. Morton
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Renk, C.M., Gupta, R.K. & Morton, D.L. Inhibition of normal allogeneic lymphocyte mitogenesis by a factor released from human tumor cells in culture. Cancer Immunol Immunother 11, 7–16 (1981). https://doi.org/10.1007/BF00205768

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  • DOI: https://doi.org/10.1007/BF00205768

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