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Stimulating effect of serum from tumor-bearing mice on lymphocyte response to mitogenic stimulation associated with protein increase in serum

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Summary

The influence of serum from mice bearing large tumors on the 3H-thymidine uptake of normal mouse spleen cells in culture following PHA and Con A stimulation was investigated.

Some sera produced a substantial increase in 3H-thymidine uptake after mitogenic stimulation, for 0.1%–5% dilution, compared with control serum or serum taken from other tumor-bearing mice.

Determination of the amount of proteins present and polyacrylamide gel electrophoretic analysis revealed higher protein values in these sera than in control serum. Sera from tumor-bearing mice that induced a decrease in 3H-thymidine uptake also contained lower amounts of protein than control serum.

Some sera, which were heated to 56° C, kept a lower stimulating action at 0.1%–1% dilution. At 5% dilution, a reverse action was observed. Sera that had a stimulating action showed a depressive action when compared with control serum heated to 56° C.

Electrophoretic analysis of serum heated to 56° C showed significant differences, principally for β-lipoproteins and γ-globulin fractions.

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  1. Research Unit of Experimental Cancerology and Radiobiology U.95 INSERM, Plateau de Brabois, 54500, Vandoeuvre-lès-Nancy, France

    D. Vaillier & J. Vaillier

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  1. D. Vaillier
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  2. J. Vaillier
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Vaillier, D., Vaillier, J. Stimulating effect of serum from tumor-bearing mice on lymphocyte response to mitogenic stimulation associated with protein increase in serum. Cancer Immunol Immunother 6, 143–149 (1979). https://doi.org/10.1007/BF00205538

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  • DOI: https://doi.org/10.1007/BF00205538

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