Summary
The purpose of this study was to characterize in vitro the systemic tumor immunity induced by a BCG-intratumoral injection in line-10 hepatocarcinoma established in the skin of inbred guinea pigs (strain 2). Macrophages from BCG-tumor-cured guinea pigs at effector to target cell ratios of 10:1 and 100:1 were cytotoxic in vitro to line-10 tumor cells, and this cytotoxicity was potentiated by autologous serum. Significant cytotoxicity of lymphocytes from BCG-tumor-cured guinea pigs could only be achieved at ratios of 10,000:1, and no effect of autologous serum could be demonstrated. Lymphocytes from both normal and BCG-tumor-cured (line-10 immune) guinea pigs had a significant cytotoxic effect on the highly antigenic line-1 cells at ratios of 1:10,000. Macrophages from both normal and line-10 immune guinea pigs were cytotoxic to line-1 target cells at ratios of 1:100. With respect to specific cytotoxicity (cytotoxicity above and beyond levels achieved with effector cells from normal animals), the only significant difference was demonstrated when line-10 served as target cells and the effector cells were isolated from BCG-tumor-cured (line-10 immune) guinea pigs.
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Evans, C. H., Ohanian, S. H., Cooney, A. M.: Tumor-specific and forssman antigens of guinea pig hepatoma cells: Comparison of tumor cells grown in vivo and in vitro. Int. J. Cancer 15, 512 (1975)
Fidler, I. J., Brodey, R. S., Bech-Neilsen, S.: In vitro immune stimulation-inhibition to spontaneous canine tumors of various histologic types. J. Immunol. 112, 1051 (1974)
Fidler, I. J.,: In vitro studies of cellular-mediated immunostimulation of tumor growth. J. Natl. Cancer Inst. 50, 1307 (1973)
Fidler, I. J.: Activation in vitro of mouse macrophages from syngeneic, allogeneic or xenogeneic lymphocyte supernatants. J. Natl. Cancer Inst. (In press), 1975
Hanna, Jr., M. G., Peters, L. C., Fidler, I. J.: The efficacy of BCG-induced tumor immunity in guinea pigs with regional and systemic malignancy. Cancer Immunology and Immunotherapy 1, 171 (1976)
Hanna, Jr., M. G., Snodgrass, M. J., Zbar, B., Rapp, H. J.: Histopathology of tumor regression after intralesional injection of Mycobacterium bovis. IV. Development of immunity to tumor cells and BCG. J. Natl. Cancer Inst. 51, 1897 (1973)
Hanna, Jr., M. G., Zbar, B., Rapp, H. J.: Histopathology of tumor regression after intralesional injection of Mycobacterium bovis. I. Tumor growth and metastasis. J. Natl. Cancer Inst. 48, 1441 (1972)
Hanna, Jr., M. G., Zbar, B., Rapp, H. J.: Histopathology of tumor regression after intralesional injection of Mycobacterium bovis. II. Comparative effects of vaccinia virus, oxazolone, and turpentine. J. Natl. Cancer Inst. 48, 1697 (1972)
Heppner, G., Henry, E., Stolbach, L., Cummings, F., McDonough, E., Calabresi, P.: Problems in the clinical use of the microcytotoxicity assay for measuring cell-mediated immunity to tumor cells. Cancer 35, 1931 (1975)
Norbury, K. C., Fidler, I. J.: In vitro tumor cell destruction by syngeneic mouse macrophages: Methods for assaying cytotoxicity. J. Immunol. 7, 109 (1975)
Rapp, H. J., Churchill, Jr., W. H., Kronman, B. S., Rolley, R. T., Hammond, W. G., Borsos, T.: Antigenicity of a new diethylnitrosoamine-induced transplantable guinea pig hepatoma: Pathology and formation of ascites variant. J. Natl. Cancer Inst. 41, 1 (1968)
Snodgrass, M. J., Hanna, Jr., M. G.: Histopathology of tumor regression after intralesional injection of Mycobacterium bovis. Ultrastructural studies of histiocyte-tumor cell interactions. Cancer Res. 33, 701 (1973)
Stutman, I.: Discrepancies between in vivo and in vitro testing of immune responses to mammary tumors in mice. Proc. AACR 16, 37 (1975)
Zbar, B., Bernstein, I. D., Bartlett, G. L., Hanna, Jr., M. G., Rapp, H. J.: Immunotherapy of cancer: Regression of intradermal tumors and prevention of growth of lymph node metastases after intralesional injection of living Mycobacterium bovis. J. Natl. Cancer Inst. 49, 119 (1972)
Zbar, B., Tanaka, T.: Immunotherapy of cancer: Regression of tumors after intralesional injection of living Mycobacterium bovis. Science 172, 271 (1971)
Zbar, B., Wapsic, H. T., Rapp, H. J., Borsos, T.; Kronman, B. S., Churchill, Jr., W. H.: Antigenic specificity of hepatomas induced in strain-2 guinea pigs by diethylnitrosoamine. J. Natl. Cancer Inst. 43, 833 (1969)
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Abbreviations used in this paper: BCG, Bacillus Calmette-Guérin; CMEM, complete minimum essential medium; cpm, counts per minute; HBSS, Hanks' balanced salt solution; i.d. intradermally; i.p., intraperitoneally; PEC, peritoneal exudate cells; SDA, superficial distal axillary; 125IdUrd, [125I]iododeoxyuridine.
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Fidler, I.J., Budmen, M.B. & Hanna, M.G. Characterization of in vitro reactivity by BCG-treated guinea pigs to syngeneic line-10 hepatocarcinoma. Cancer Immunol Immunother 1, 179–186 (1976). https://doi.org/10.1007/BF00205463
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DOI: https://doi.org/10.1007/BF00205463