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Generation of killer lymphocytes in vitro against human autologous leukemia cells with leukemic blasts and BCG extract

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Summary

Acute lymphoblastic leukemia patients under 18 years of age were studied to determine the ability of their remission lymphocytes to kill autologous leukemic blasts (ALB) following in vitro sensitization with their leukemic cells and/or soluble extract of BCG (BCG-SE). Remission lymphocytes, when cultured together with the mitomycin-treated ALB, became significantly lytic for ALB but not for autologous remission lymphocytes. The ALB were usually immunogenic at low concentrations and no cytotoxic lymphocytes were generated at a ratio of 1:1 of responding lymphocytes to stimulating leukemic cells. T-leukemic cells appeared to immunize more effectively than null-cell leukemic cells. In some cases, when ALB alone could not generate killer lymphocytes (KL) the combination of ALB and BCG-SE induced more intense cytotoxicity than was induced by BCG-SE alone. In a few other cases, the addition of BCG-SE to mixed lymphocyte leukemic cell cultures potentiated the immunization of lymphocytes by leukemic cells. Inhibition of cytotoxicity induction was noted in one case when remission lymphocytes were cultured together with ALB and BCG-SE. Leukemic cellssensitized lymphocytes from some cancer patients and normal persons were cytotoxic to several but not all patients' leukemic cells tested. Nylon wool-nonadherent, non-E-rosette-forming, and E-rosette-forming cells became cytotoxic following in vitro stimulation with autologous leukemic cells.

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Authors and Affiliations

  1. The Children's Hospital, 1056 East Nineteenth Avenue, 80218, Denver, Colorado, USA

    Brahma Sharma & Lorrie Furman Odom

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  1. Brahma Sharma
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  2. Lorrie Furman Odom
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Sharma, B., Odom, L.F. Generation of killer lymphocytes in vitro against human autologous leukemia cells with leukemic blasts and BCG extract. Cancer Immunol Immunother 7, 99–105 (1979). https://doi.org/10.1007/BF00205331

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  • DOI: https://doi.org/10.1007/BF00205331

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